(Linear) (Six-base) MAPSORT of: KIEE4081.seq  Check: 9464  from: 1  to: 1284



KIEE4081.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158    1284 
   Size:        158    1126
 
AflIII A'CryG_T

Cuts at:      0     561    1284 
   Size:        561     723
 
AlwNI CAG_nnn'CTG

Cuts at:      0      23    1284 
   Size:         23    1261
 
ApoI r'AATT_y

Cuts at:      0     230     937    1284 
   Size:        230     707     347

  Fragments arranged by size: 

                 707     347     230
 
AvaI C'yCGr_G

Cuts at:      0     504    1284 
   Size:        504     780
 
AvrII C'CTAG_G

Cuts at:      0     352    1284 
   Size:        352     932
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652    1201    1284 
   Size:        652     549      83

  Fragments arranged by size: 

                 652     549      83
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1124    1284 
   Size:       1124     160
 
BseYI C'CCAG_C

Cuts at:      0    1145    1284 
   Size:       1145     139
 
BsiHKAI G_wGCw'C

Cuts at:      0     467     630    1104    1284 
   Size:        467     163     474     180

  Fragments arranged by size: 

                 474     467     180     163
 
Bsp1286I G_dGCh'C

Cuts at:      0     467     630    1104    1284 
   Size:        467     163     474     180

  Fragments arranged by size: 

                 474     467     180     163
 
BspHI T'CATG_A

Cuts at:      0    1269    1284 
   Size:       1269      15
 
BsrFI r'CCGG_y

Cuts at:      0    1004    1284 
   Size:       1004     280
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     735    1284 
   Size:        735     549
 
BtsI GCAGTG_nn'

Cuts at:      0    1225    1284 
   Size:       1225      59
 
EaeI y'GGCC_r

Cuts at:      0      14     921    1006    1284 
   Size:         14     907      85     278

  Fragments arranged by size: 

                 907     278      85      14
 
EarI CTCTTCn'nnn_

Cuts at:      0     296     308    1284 
   Size:        296      12     976

  Fragments arranged by size: 

                 976     296      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158    1284 
   Size:        158    1126
 
EcoRI G'AATT_C

Cuts at:      0     230    1284 
   Size:        230    1054
 
EcoRV GAT'ATC

Cuts at:      0     359    1284 
   Size:        359     925
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     826    1284 
   Size:        826     458
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     994    1026    1264    1284 
   Size:        316      32     646      32     238      20

  Fragments arranged by size: 

                 646     316     238      32      32      20
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     725    1138    1284 
   Size:        138     587     413     146

  Fragments arranged by size: 

                 587     413     146     138
 
MscI TGG'CCA

Cuts at:      0      16     923    1284 
   Size:         16     907     361

  Fragments arranged by size: 

                 907     361      16
 
MslI CAynn'nnrTG

Cuts at:      0    1205    1284 
   Size:       1205      79
 
NspI r_CATG'y

Cuts at:      0     565    1284 
   Size:        565     719
 
PciI A'CATG_T

Cuts at:      0     561    1284 
   Size:        561     723
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     837    1284 
   Size:        837     447
 
PsiI TTA'TAA

Cuts at:      0     959    1284 
   Size:        959     325
 
SfcI C'TryA_G

Cuts at:      0    1215    1284 
   Size:       1215      69
 
SmlI C'TyrA_G

Cuts at:      0     631    1180    1284 
   Size:        631     549     104

  Fragments arranged by size: 

                 631     549     104
 
SpeI A'CTAG_T

Cuts at:      0     945    1284 
   Size:        945     339
 
SspI AAT'ATT

Cuts at:      0     278    1284 
   Size:        278    1006
 
StuI AGG'CCT

Cuts at:      0     545    1284 
   Size:        545     739
 
StyI C'CwwG_G

Cuts at:      0     123     352    1284 
   Size:        123     229     932

  Fragments arranged by size: 

                 932     229     123
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1155    1187    1284 
   Size:       1155      32      97

  Fragments arranged by size: 

                1155      97      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     210    1284 
   Size:        210    1074
 
XmnI GAAnn'nnTTC

Cuts at:      0     766    1284 
   Size:        766     518
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI    AvrII    BpuEI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspHI    BsrFI    BstXI     BtsI     EaeI
     EarI  Eco57MI    EcoRI    EcoRV     FalI    Hin4I     MmeI     MscI
     MslI     NspI     PciI    PflMI     PsiI     SfcI     SmlI     SpeI
     SspI     StuI     StyI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI