Restriction map for
pFN21AE4077
(Linear) (Six-base) MAPSORT of: KIEE4077.seq Check: 117 from: 1 to: 1209
KIEE4077.orf
With 182 enzymes: SgfI *
March 22, 2012 17:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 295 1209
Size: 295 914
AclI AA'CG_TT
Cuts at: 0 896 1209
Size: 896 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1115 1209
Size: 97 1018 94
Fragments arranged by size:
1018 97 94
AleI CACnn'nnGTG
Cuts at: 0 162 1209
Size: 162 1047
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 924 956 1209
Size: 924 32 253
Fragments arranged by size:
924 253 32
ApoI r'AATT_y
Cuts at: 0 152 745 827 917 973 983 1183 1209
Size: 152 593 82 90 56 10 200 26
Fragments arranged by size:
593 200 152 90 82 56 26 10
AseI AT'TA_AT
Cuts at: 0 641 703 1009 1209
Size: 641 62 306 200
Fragments arranged by size:
641 306 200 62
AvaI C'yCGr_G
Cuts at: 0 836 1209
Size: 836 373
BbsI GAAGACnn'nnnn_
Cuts at: 0 272 1209
Size: 272 937
BglII A'GATC_T
Cuts at: 0 474 785 1209
Size: 474 311 424
Fragments arranged by size:
474 424 311
Bme1580I G_kGCm'C
Cuts at: 0 169 1209
Size: 169 1040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1209
Size: 541 668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 1209
Size: 145 1064
BsaI GGTCTCn'nnnn_
Cuts at: 0 135 1209
Size: 135 1074
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 422 1209
Size: 422 787
Bsp1286I G_dGCh'C
Cuts at: 0 169 1209
Size: 169 1040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 295 1209
Size: 295 914
BsrDI GCAATG_nn'
Cuts at: 0 354 1209
Size: 354 855
BsrGI T'GTAC_A
Cuts at: 0 780 1209
Size: 780 429
BssSI C'ACGA_G
Cuts at: 0 59 1209
Size: 59 1150
BstYI r'GATC_y
Cuts at: 0 54 474 785 1209
Size: 54 420 311 424
Fragments arranged by size:
424 420 311 54
Bsu36I CC'TnA_GG
Cuts at: 0 809 1209
Size: 809 400
DraI TTT'AAA
Cuts at: 0 980 989 1209
Size: 980 9 220
Fragments arranged by size:
980 220 9
EarI CTCTTCn'nnn_
Cuts at: 0 11 725 1209
Size: 11 714 484
Fragments arranged by size:
714 484 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 541 1115 1209
Size: 97 444 574 94
Fragments arranged by size:
574 444 97 94
EcoRI G'AATT_C
Cuts at: 0 152 1209
Size: 152 1057
HincII GTy'rAC
Cuts at: 0 23 373 455 1209
Size: 23 350 82 754
Fragments arranged by size:
754 350 82 23
HpaI GTT'AAC
Cuts at: 0 23 1209
Size: 23 1186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1209
Size: 223 986
MslI CAynn'nnrTG
Cuts at: 0 162 1209
Size: 162 1047
NdeI CA'TA_TG
Cuts at: 0 34 1209
Size: 34 1175
NspI r_CATG'y
Cuts at: 0 781 1209
Size: 781 428
PspXI vC'TCGA_Gb
Cuts at: 0 836 1209
Size: 836 373
SfcI C'TryA_G
Cuts at: 0 389 1209
Size: 389 820
SmlI C'TyrA_G
Cuts at: 0 160 836 1209
Size: 160 676 373
Fragments arranged by size:
676 373 160
SspI AAT'ATT
Cuts at: 0 851 1209
Size: 851 358
StyI C'CwwG_G
Cuts at: 0 534 1209
Size: 534 675
TatI w'GTAC_w
Cuts at: 0 63 780 1209
Size: 63 717 429
Fragments arranged by size:
717 429 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 1209
Size: 1174 32 3
Fragments arranged by size:
1174 32 3
XhoI C'TCGA_G
Cuts at: 0 836 1209
Size: 836 373
XmnI GAAnn'nnTTC
Cuts at: 0 107 551 1209
Size: 107 444 658
Fragments arranged by size:
658 444 107
Enzymes that do cut:
AarI AclI AcuI AleI AloI ApoI AseI AvaI
BbsI BglII Bme1580I BpmI BpuEI BsaI BseRI Bsp1286I
BspMI BsrDI BsrGI BssSI BstYI Bsu36I DraI EarI
Eco57MI EcoRI HincII HpaI MmeI MslI NdeI NspI
PspXI SfcI SmlI SspI StyI TatI TstI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII Hin4I HindIII KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI
XcmI XmaI ZraI