Restriction map for
pFN21AE4074
(Linear) (Six-base) MAPSORT of: KIEE4074.seq Check: 4797 from: 1 to: 1200
KIEE4074.orf
With 182 enzymes: SgfI *
March 22, 2012 17:19 ..
AatII G_ACGT'C
Cuts at: 0 1001 1200
Size: 1001 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1200
Size: 67 1133
AflIII A'CryG_T
Cuts at: 0 332 1200
Size: 332 868
AlwNI CAG_nnn'CTG
Cuts at: 0 219 1098 1167 1200
Size: 219 879 69 33
Fragments arranged by size:
879 219 69 33
ApaI G_GGCC'C
Cuts at: 0 710 1200
Size: 710 490
BamHI G'GATC_C
Cuts at: 0 638 1200
Size: 638 562
BanI G'GyrC_C
Cuts at: 0 122 1200
Size: 122 1078
BanII G_rGCy'C
Cuts at: 0 56 550 710 905 1187 1200
Size: 56 494 160 195 282 13
Fragments arranged by size:
494 282 195 160 56 13
BclI T'GATC_A
Cuts at: 0 230 1200
Size: 230 970
Bme1580I G_kGCm'C
Cuts at: 0 394 710 1200
Size: 394 316 490
Fragments arranged by size:
490 394 316
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 630 1200
Size: 207 423 570
Fragments arranged by size:
570 423 207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 1200
Size: 186 1014
BsaAI yAC'GTr
Cuts at: 0 335 1200
Size: 335 865
BsaHI Gr'CG_yC
Cuts at: 0 998 1200
Size: 998 202
BsaWI w'CCGG_w
Cuts at: 0 380 524 1200
Size: 380 144 676
Fragments arranged by size:
676 380 144
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 1042 1045 1200
Size: 427 615 3 155
Fragments arranged by size:
615 427 155 3
BsiHKAI G_wGCw'C
Cuts at: 0 550 758 905 1187 1200
Size: 550 208 147 282 13
Fragments arranged by size:
550 282 208 147 13
Bsp1286I G_dGCh'C
Cuts at: 0 56 394 550 710 758 905 1187 1200
Size: 56 338 156 160 48 147 282 13
Fragments arranged by size:
338 282 160 156 147 56 48 13
BspEI T'CCGG_A
Cuts at: 0 380 1200
Size: 380 820
BsrBI CCG'CTC
Cuts at: 0 636 1200
Size: 636 564
BsrDI GCAATG_nn'
Cuts at: 0 245 1200
Size: 245 955
BsrFI r'CCGG_y
Cuts at: 0 323 1200
Size: 323 877
BstXI CCAn_nnnn'nTGG
Cuts at: 0 795 1200
Size: 795 405
BstYI r'GATC_y
Cuts at: 0 638 803 1163 1200
Size: 638 165 360 37
Fragments arranged by size:
638 360 165 37
Bsu36I CC'TnA_GG
Cuts at: 0 220 1200
Size: 220 980
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 657 1200
Size: 657 543
BtsI GCAGTG_nn'
Cuts at: 0 847 964 1200
Size: 847 117 236
Fragments arranged by size:
847 236 117
DrdI GACnn_nn'nnGTC
Cuts at: 0 376 1200
Size: 376 824
EaeI y'GGCC_r
Cuts at: 0 188 797 1200
Size: 188 609 403
Fragments arranged by size:
609 403 188
EarI CTCTTCn'nnn_
Cuts at: 0 1027 1200
Size: 1027 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 128 1200
Size: 128 1072
EcoICRI GAG'CTC
Cuts at: 0 548 903 1185 1200
Size: 548 355 282 15
Fragments arranged by size:
548 355 282 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1200
Size: 67 1133
EcoO109I rG'GnC_Cy
Cuts at: 0 706 707 1200
Size: 706 1 493
Fragments arranged by size:
706 493 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 159 1200
Size: 159 1041
MfeI C'AATT_G
Cuts at: 0 59 1200
Size: 59 1141
MscI TGG'CCA
Cuts at: 0 190 1200
Size: 190 1010
MslI CAynn'nnrTG
Cuts at: 0 265 541 1075 1200
Size: 265 276 534 125
Fragments arranged by size:
534 276 265 125
MspA1I CmG'CkG
Cuts at: 0 956 1200
Size: 956 244
PfoI T'CCnGG_A
Cuts at: 0 35 443 806 1085 1200
Size: 35 408 363 279 115
Fragments arranged by size:
408 363 279 115 35
PspOMI G'GGCC_C
Cuts at: 0 706 1200
Size: 706 494
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 199 231 1200
Size: 199 32 969
Fragments arranged by size:
969 199 32
PstI C_TGCA'G
Cuts at: 0 365 1136 1172 1200
Size: 365 771 36 28
Fragments arranged by size:
771 365 36 28
SacI G_AGCT'C
Cuts at: 0 550 905 1187 1200
Size: 550 355 282 13
Fragments arranged by size:
550 355 282 13
SfcI C'TryA_G
Cuts at: 0 361 1132 1168 1200
Size: 361 771 36 32
Fragments arranged by size:
771 361 36 32
StyI C'CwwG_G
Cuts at: 0 158 251 1178 1200
Size: 158 93 927 22
Fragments arranged by size:
927 158 93 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 1200
Size: 253 947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 513 545 1200
Size: 513 32 655
Fragments arranged by size:
655 513 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 1200
Size: 258 942
XmnI GAAnn'nnTTC
Cuts at: 0 470 1200
Size: 470 730
ZraI GAC'GTC
Cuts at: 0 999 1200
Size: 999 201
Enzymes that do cut:
AatII AcuI AflIII AlwNI ApaI BamHI BanI BanII
BclI Bme1580I BmrI BplI BsaAI BsaHI BsaWI BseRI
BsiHKAI Bsp1286I BspEI BsrBI BsrDI BsrFI BstXI BstYI
Bsu36I BtgZI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI MfeI MscI MslI MspA1I PfoI
PspOMI PsrI PstI SacI SfcI StyI TaqII TstI
XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI BpmI Bpu10I
BpuEI BsaI BsaBI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspHI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgI ClaI CspCI DraI DraIII
EagI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI
XmaI