Restriction map for
pFN21AE4072
(Linear) (Six-base) MAPSORT of: KIEE4072.seq Check: 7142 from: 1 to: 1137
KIEE4072.orf
With 182 enzymes: SgfI *
March 22, 2012 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 564 1137
Size: 564 573
AatII G_ACGT'C
Cuts at: 0 329 1137
Size: 329 808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 921 1137
Size: 280 641 216
Fragments arranged by size:
641 280 216
ApaI G_GGCC'C
Cuts at: 0 172 217 249 1137
Size: 172 45 32 888
Fragments arranged by size:
888 172 45 32
ApaLI G'TGCA_C
Cuts at: 0 401 1137
Size: 401 736
ApoI r'AATT_y
Cuts at: 0 100 1108 1137
Size: 100 1008 29
Fragments arranged by size:
1008 100 29
AvaI C'yCGr_G
Cuts at: 0 152 171 437 483 490 586 658 842
Size: 152 19 266 46 7 96 72 184
Cuts at: 842 1137
Size: 295
Fragments arranged by size:
295 266 184 152 96 72 46 19
7
BanI G'GyrC_C
Cuts at: 0 273 594 873 1137
Size: 273 321 279 264
Fragments arranged by size:
321 279 273 264
BanII G_rGCy'C
Cuts at: 0 172 217 249 578 1097 1137
Size: 172 45 32 329 519 40
Fragments arranged by size:
519 329 172 45 40 32
BbeI G_GCGC'C
Cuts at: 0 598 877 1137
Size: 598 279 260
Fragments arranged by size:
598 279 260
BbsI GAAGACnn'nnnn_
Cuts at: 0 992 1137
Size: 992 145
BbvCI CC'TCA_GC
Cuts at: 0 497 1137
Size: 497 640
BglI GCCn_nnn'nGGC
Cuts at: 0 239 661 694 819 1137
Size: 239 422 33 125 318
Fragments arranged by size:
422 318 239 125 33
BglII A'GATC_T
Cuts at: 0 714 1137
Size: 714 423
Bme1580I G_kGCm'C
Cuts at: 0 16 126 172 217 249 276 340 405
Size: 16 110 46 45 32 27 64 65
Cuts at: 405 1137
Size: 732
Fragments arranged by size:
732 110 65 64 46 45 32 27
16
BmrI ACTGGGnnnn_n'
Cuts at: 0 196 527 1137
Size: 196 331 610
Fragments arranged by size:
610 331 196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1006 1137
Size: 650 356 131
Fragments arranged by size:
650 356 131
Bpu10I CC'TnA_GC
Cuts at: 0 497 1091 1137
Size: 497 594 46
Fragments arranged by size:
594 497 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 493 986 1137
Size: 493 493 151
Fragments arranged by size:
493 493 151
BsaHI Gr'CG_yC
Cuts at: 0 40 157 326 595 874 1137
Size: 40 117 169 269 279 263
Fragments arranged by size:
279 269 263 169 117 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 902 932 1137
Size: 25 877 30 205
Fragments arranged by size:
877 205 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 934 1137
Size: 367 567 203
Fragments arranged by size:
567 367 203
BseYI C'CCAG_C
Cuts at: 0 242 507 1137
Size: 242 265 630
Fragments arranged by size:
630 265 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1137
Size: 893 244
BsiEI CG_ry'CG
Cuts at: 0 281 604 653 704 738 754 823 1137
Size: 281 323 49 51 34 16 69 314
Fragments arranged by size:
323 314 281 69 51 49 34 16
BsiHKAI G_wGCw'C
Cuts at: 0 405 934 1137
Size: 405 529 203
Fragments arranged by size:
529 405 203
Bsp1286I G_dGCh'C
Cuts at: 0 16 126 172 217 249 276 340 405
Size: 16 110 46 45 32 27 64 65
Cuts at: 405 578 934 1097 1137
Size: 173 356 163 40
Fragments arranged by size:
356 173 163 110 65 64 46 45
40 32 27 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 564 1137
Size: 564 573
BsrBI CCG'CTC
Cuts at: 0 819 1137
Size: 819 318
BsrFI r'CCGG_y
Cuts at: 0 36 310 648 749 837 863 870 1002
Size: 36 274 338 101 88 26 7 132
Cuts at: 1002 1137
Size: 135
Fragments arranged by size:
338 274 135 132 101 88 36 26
7
BssSI C'ACGA_G
Cuts at: 0 927 1137
Size: 927 210
BstYI r'GATC_y
Cuts at: 0 714 1137
Size: 714 423
Bsu36I CC'TnA_GG
Cuts at: 0 218 524 1137
Size: 218 306 613
Fragments arranged by size:
613 306 218
BtgI C'CryG_G
Cuts at: 0 345 578 598 1137
Size: 345 233 20 539
Fragments arranged by size:
539 345 233 20
BtsI GCAGTG_nn'
Cuts at: 0 38 1137
Size: 38 1099
EaeI y'GGCC_r
Cuts at: 0 601 615 650 671 686 701 820 1137
Size: 601 14 35 21 15 15 119 317
Fragments arranged by size:
601 317 119 35 21 15 15 14
EagI C'GGCC_G
Cuts at: 0 601 650 701 820 1137
Size: 601 49 51 119 317
Fragments arranged by size:
601 317 119 51 49
EarI CTCTTCn'nnn_
Cuts at: 0 353 1137
Size: 353 784
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 380 709 736 1137
Size: 380 329 27 401
Fragments arranged by size:
401 380 329 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 650 921 1006 1137
Size: 280 370 271 85 131
Fragments arranged by size:
370 280 271 131 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 830 1137
Size: 830 307
EcoO109I rG'GnC_Cy
Cuts at: 0 92 175 213 214 222 246 583 1078
Size: 92 83 38 1 8 24 337 495
Cuts at: 1078 1137
Size: 59
Fragments arranged by size:
495 337 92 83 59 38 24 8
1
EcoRI G'AATT_C
Cuts at: 0 100 1137
Size: 100 1037
HaeII r_GCGC'y
Cuts at: 0 598 877 1137
Size: 598 279 260
Fragments arranged by size:
598 279 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1062 1094 1137
Size: 1062 32 43
Fragments arranged by size:
1062 43 32
KasI G'GCGC_C
Cuts at: 0 594 873 1137
Size: 594 279 264
Fragments arranged by size:
594 279 264
MspA1I CmG'CkG
Cuts at: 0 111 347 416 580 600 670 1056 1084
Size: 111 236 69 164 20 70 386 28
Cuts at: 1084 1137
Size: 53
Fragments arranged by size:
386 236 164 111 70 69 53 28
20
NaeI GCC'GGC
Cuts at: 0 38 312 650 839 865 872 1004 1137
Size: 38 274 338 189 26 7 132 133
Fragments arranged by size:
338 274 189 133 132 38 26 7
NarI GG'CG_CC
Cuts at: 0 595 874 1137
Size: 595 279 263
Fragments arranged by size:
595 279 263
NgoMIV G'CCGG_C
Cuts at: 0 36 310 648 837 863 870 1002 1137
Size: 36 274 338 189 26 7 132 135
Fragments arranged by size:
338 274 189 135 132 36 26 7
NotI GC'GGCC_GC
Cuts at: 0 820 1137
Size: 820 317
PpuMI rG'GwC_Cy
Cuts at: 0 92 583 1078 1137
Size: 92 491 495 59
Fragments arranged by size:
495 491 92 59
PspOMI G'GGCC_C
Cuts at: 0 168 213 245 1137
Size: 168 45 32 892
Fragments arranged by size:
892 168 45 32
PvuI CG_AT'CG
Cuts at: 0 738 1137
Size: 738 399
SacII CC_GC'GG
Cuts at: 0 348 581 601 1137
Size: 348 233 20 536
Fragments arranged by size:
536 348 233 20
SanDI GG'GwC_CC
Cuts at: 0 92 583 1078 1137
Size: 92 491 495 59
Fragments arranged by size:
495 491 92 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 819 1137
Size: 819 318
SfoI GGC'GCC
Cuts at: 0 596 875 1137
Size: 596 279 262
Fragments arranged by size:
596 279 262
SgrAI Cr'CCGG_yG
Cuts at: 0 310 1137
Size: 310 827
SmaI CCC'GGG
Cuts at: 0 154 173 485 588 1137
Size: 154 19 312 103 549
Fragments arranged by size:
549 312 154 103 19
SmlI C'TyrA_G
Cuts at: 0 490 1137
Size: 490 647
StyI C'CwwG_G
Cuts at: 0 785 1137
Size: 785 352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 902 934 1137
Size: 27 875 32 203
Fragments arranged by size:
875 203 32 27
XhoI C'TCGA_G
Cuts at: 0 490 1137
Size: 490 647
XmaI C'CCGG_G
Cuts at: 0 152 171 483 586 1137
Size: 152 19 312 103 551
Fragments arranged by size:
551 312 152 103 19
XmnI GAAnn'nnTTC
Cuts at: 0 778 1137
Size: 778 359
ZraI GAC'GTC
Cuts at: 0 327 1137
Size: 327 810
Enzymes that do cut:
AarI AatII AcuI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstYI
Bsu36I BtgI BtsI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I KasI MspA1I NaeI
NarI NgoMIV NotI PpuMI PspOMI PvuI SacII SanDI
SfiI SfoI SgrAI SmaI SmlI StyI TstI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XcmI