Restriction map for
pFN21AE4069
(Linear) (Six-base) MAPSORT of: KIEE4069.seq Check: 9442 from: 1 to: 1104
KIEE4069.orf
With 182 enzymes: SgfI *
March 22, 2012 17:18 ..
Acc65I G'GTAC_C
Cuts at: 0 332 1104
Size: 332 772
AleI CACnn'nnGTG
Cuts at: 0 190 862 1104
Size: 190 672 242
Fragments arranged by size:
672 242 190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 880 1104
Size: 880 224
AlwNI CAG_nnn'CTG
Cuts at: 0 906 1104
Size: 906 198
ApoI r'AATT_y
Cuts at: 0 842 999 1104
Size: 842 157 105
Fragments arranged by size:
842 157 105
BanI G'GyrC_C
Cuts at: 0 332 1104
Size: 332 772
BanII G_rGCy'C
Cuts at: 0 567 579 703 1104
Size: 567 12 124 401
Fragments arranged by size:
567 401 124 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 696 1081 1104
Size: 696 385 23
Fragments arranged by size:
696 385 23
BbvCI CC'TCA_GC
Cuts at: 0 226 1104
Size: 226 878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 775 1104
Size: 692 83 329
Fragments arranged by size:
692 329 83
Bpu10I CC'TnA_GC
Cuts at: 0 226 1104
Size: 226 878
BsaBI GATnn'nnATC
Cuts at: 0 488 1104
Size: 488 616
BsaWI w'CCGG_w
Cuts at: 0 162 1104
Size: 162 942
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 695 1022 1039 1104
Size: 31 664 327 17 65
Fragments arranged by size:
664 327 65 31 17
BseYI C'CCAG_C
Cuts at: 0 86 144 1104
Size: 86 58 960
Fragments arranged by size:
960 86 58
BsiHKAI G_wGCw'C
Cuts at: 0 579 1104
Size: 579 525
Bsp1286I G_dGCh'C
Cuts at: 0 567 579 703 1104
Size: 567 12 124 401
Fragments arranged by size:
567 401 124 12
BspEI T'CCGG_A
Cuts at: 0 162 1104
Size: 162 942
BspHI T'CATG_A
Cuts at: 0 470 579 1104
Size: 470 109 525
Fragments arranged by size:
525 470 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 789 1104
Size: 789 315
BsrBI CCG'CTC
Cuts at: 0 21 1104
Size: 21 1083
BsrDI GCAATG_nn'
Cuts at: 0 626 1104
Size: 626 478
BstEII G'GTnAC_C
Cuts at: 0 780 1104
Size: 780 324
Bsu36I CC'TnA_GG
Cuts at: 0 1005 1104
Size: 1005 99
BtgI C'CryG_G
Cuts at: 0 1052 1104
Size: 1052 52
DraI TTT'AAA
Cuts at: 0 656 1104
Size: 656 448
EaeI y'GGCC_r
Cuts at: 0 45 1104
Size: 45 1059
EarI CTCTTCn'nnn_
Cuts at: 0 754 1104
Size: 754 350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 250 1104
Size: 28 222 854
Fragments arranged by size:
854 222 28
EcoICRI GAG'CTC
Cuts at: 0 577 1104
Size: 577 527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 775 1104
Size: 692 83 329
Fragments arranged by size:
692 329 83
EcoO109I rG'GnC_Cy
Cuts at: 0 131 1104
Size: 131 973
HaeII r_GCGC'y
Cuts at: 0 142 199 1104
Size: 142 57 905
Fragments arranged by size:
905 142 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1104
Size: 595 32 477
Fragments arranged by size:
595 477 32
KpnI G_GTAC'C
Cuts at: 0 336 1104
Size: 336 768
MslI CAynn'nnrTG
Cuts at: 0 190 862 1104
Size: 190 672 242
Fragments arranged by size:
672 242 190
MspA1I CmG'CkG
Cuts at: 0 10 171 1104
Size: 10 161 933
Fragments arranged by size:
933 161 10
PfoI T'CCnGG_A
Cuts at: 0 714 1104
Size: 714 390
PpuMI rG'GwC_Cy
Cuts at: 0 131 1104
Size: 131 973
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 953 985 1104
Size: 953 32 119
Fragments arranged by size:
953 119 32
PstI C_TGCA'G
Cuts at: 0 729 1104
Size: 729 375
PvuII CAG'CTG
Cuts at: 0 171 1104
Size: 171 933
SacI G_AGCT'C
Cuts at: 0 579 1104
Size: 579 525
SanDI GG'GwC_CC
Cuts at: 0 131 1104
Size: 131 973
SapI GCTCTTCn'nnn_
Cuts at: 0 754 1104
Size: 754 350
ScaI AGT'ACT
Cuts at: 0 587 628 1104
Size: 587 41 476
Fragments arranged by size:
587 476 41
SfcI C'TryA_G
Cuts at: 0 725 992 1104
Size: 725 267 112
Fragments arranged by size:
725 267 112
StyI C'CwwG_G
Cuts at: 0 48 373 1104
Size: 48 325 731
Fragments arranged by size:
731 325 48
TatI w'GTAC_w
Cuts at: 0 585 626 1104
Size: 585 41 478
Fragments arranged by size:
585 478 41
Tth111I GACn'n_nGTC
Cuts at: 0 286 1104
Size: 286 818
XmnI GAAnn'nnTTC
Cuts at: 0 399 587 1104
Size: 399 188 517
Fragments arranged by size:
517 399 188
Enzymes that do cut:
Acc65I AleI AlfI AlwNI ApoI BanI BanII BbsI
BbvCI BpmI Bpu10I BsaBI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BstEII Bsu36I
BtgI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I KpnI MslI MspA1I PfoI PpuMI PsrI
PstI PvuII SacI SanDI SapI ScaI SfcI StyI
TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaHI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI
RsrII SacII SalI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI XbaI XcmI XhoI XmaI
ZraI