Restriction map for
pFN21AE4063
(Linear) (Six-base) MAPSORT of: KIEE4063.seq Check: 7790 from: 1 to: 1020
KIEE4063.orf
With 182 enzymes: SgfI *
March 22, 2012 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 912 1020
Size: 912 108
AatII G_ACGT'C
Cuts at: 0 775 1020
Size: 775 245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1020
Size: 538 482
AfeI AGC'GCT
Cuts at: 0 988 1020
Size: 988 32
AlwNI CAG_nnn'CTG
Cuts at: 0 322 1020
Size: 322 698
ApaI G_GGCC'C
Cuts at: 0 186 240 895 1020
Size: 186 54 655 125
Fragments arranged by size:
655 186 125 54
AvaI C'yCGr_G
Cuts at: 0 707 886 1020
Size: 707 179 134
Fragments arranged by size:
707 179 134
BanI G'GyrC_C
Cuts at: 0 957 1020
Size: 957 63
BanII G_rGCy'C
Cuts at: 0 186 240 307 806 895 1020
Size: 186 54 67 499 89 125
Fragments arranged by size:
499 186 125 89 67 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 573 1020
Size: 573 447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 747 781 877 911 1020
Size: 747 34 96 34 109
Fragments arranged by size:
747 109 96 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 441 1020
Size: 441 579
BglI GCCn_nnn'nGGC
Cuts at: 0 62 181 890 1020
Size: 62 119 709 130
Fragments arranged by size:
709 130 119 62
BlpI GC'TnA_GC
Cuts at: 0 751 1020
Size: 751 269
Bme1580I G_kGCm'C
Cuts at: 0 186 240 895 1020
Size: 186 54 655 125
Fragments arranged by size:
655 186 125 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 394 742 975 1020
Size: 68 326 348 233 45
Fragments arranged by size:
348 326 233 68 45
Bpu10I CC'TnA_GC
Cuts at: 0 306 1020
Size: 306 714
BsaI GGTCTCn'nnnn_
Cuts at: 0 412 616 1020
Size: 412 204 404
Fragments arranged by size:
412 404 204
BsaHI Gr'CG_yC
Cuts at: 0 91 566 772 1020
Size: 91 475 206 248
Fragments arranged by size:
475 248 206 91
BsaWI w'CCGG_w
Cuts at: 0 155 432 1020
Size: 155 277 588
Fragments arranged by size:
588 277 155
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 620 650 1020
Size: 620 30 370
Fragments arranged by size:
620 370 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 739 1020
Size: 739 281
BseYI C'CCAG_C
Cuts at: 0 643 1020
Size: 643 377
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 890 1020
Size: 769 121 130
Fragments arranged by size:
769 130 121
BsiEI CG_ry'CG
Cuts at: 0 597 779 1020
Size: 597 182 241
Fragments arranged by size:
597 241 182
BsiHKAI G_wGCw'C
Cuts at: 0 385 426 806 1019 1020
Size: 385 41 380 213 1
Fragments arranged by size:
385 380 213 41 1
BsmBI CGTCTCn'nnnn_
Cuts at: 0 453 763 1020
Size: 453 310 257
Fragments arranged by size:
453 310 257
Bsp1286I G_dGCh'C
Cuts at: 0 186 240 307 385 426 806 895 1019
Size: 186 54 67 78 41 380 89 124
Cuts at: 1019 1020
Size: 1
Fragments arranged by size:
380 186 124 89 78 67 54 41
1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 912 1020
Size: 912 108
BsrBI CCG'CTC
Cuts at: 0 161 368 704 1020
Size: 161 207 336 316
Fragments arranged by size:
336 316 207 161
BssSI C'ACGA_G
Cuts at: 0 795 1020
Size: 795 225
BstAPI GCAn_nnn'nTGC
Cuts at: 0 322 1020
Size: 322 698
BstEII G'GTnAC_C
Cuts at: 0 164 1020
Size: 164 856
BstYI r'GATC_y
Cuts at: 0 827 1020
Size: 827 193
Bsu36I CC'TnA_GG
Cuts at: 0 269 1020
Size: 269 751
BtsI GCAGTG_nn'
Cuts at: 0 546 781 1020
Size: 546 235 239
Fragments arranged by size:
546 239 235
EaeI y'GGCC_r
Cuts at: 0 262 740 776 1020
Size: 262 478 36 244
Fragments arranged by size:
478 262 244 36
EagI C'GGCC_G
Cuts at: 0 776 1020
Size: 776 244
EarI CTCTTCn'nnn_
Cuts at: 0 189 811 1020
Size: 189 622 209
Fragments arranged by size:
622 209 189
EcoICRI GAG'CTC
Cuts at: 0 804 1020
Size: 804 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 394 538 742 975 1020
Size: 68 326 144 204 233 45
Fragments arranged by size:
326 233 204 144 68 45
EcoO109I rG'GnC_Cy
Cuts at: 0 182 183 891 892 1020
Size: 182 1 708 1 128
Fragments arranged by size:
708 182 128 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 711 1020
Size: 711 309
HaeII r_GCGC'y
Cuts at: 0 990 1020
Size: 990 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 858 890 1020
Size: 858 32 130
Fragments arranged by size:
858 130 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 660 1020
Size: 131 529 360
Fragments arranged by size:
529 360 131
MscI TGG'CCA
Cuts at: 0 264 1020
Size: 264 756
MslI CAynn'nnrTG
Cuts at: 0 22 28 355 469 1020
Size: 22 6 327 114 551
Fragments arranged by size:
551 327 114 22 6
PasI CC'CwG_GG
Cuts at: 0 178 1020
Size: 178 842
PshAI GACnn'nnGTC
Cuts at: 0 493 1020
Size: 493 527
PspOMI G'GGCC_C
Cuts at: 0 182 236 891 1020
Size: 182 54 655 129
Fragments arranged by size:
655 182 129 54
SacI G_AGCT'C
Cuts at: 0 806 1020
Size: 806 214
SapI GCTCTTCn'nnn_
Cuts at: 0 811 1020
Size: 811 209
SexAI A'CCwGG_T
Cuts at: 0 953 1020
Size: 953 67
SfcI C'TryA_G
Cuts at: 0 245 1020
Size: 245 775
SmaI CCC'GGG
Cuts at: 0 709 1020
Size: 709 311
StuI AGG'CCT
Cuts at: 0 274 1020
Size: 274 746
StyI C'CwwG_G
Cuts at: 0 299 1020
Size: 299 721
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 611 1020
Size: 611 409
XmaI C'CCGG_G
Cuts at: 0 707 1020
Size: 707 313
ZraI GAC'GTC
Cuts at: 0 773 1020
Size: 773 247
Enzymes that do cut:
AarI AatII AcuI AfeI AlwNI ApaI AvaI BanI
BanII BbsI BcgI BciVI BglI BlpI Bme1580I BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BssSI BstAPI
BstEII BstYI Bsu36I BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I MmeI MscI MslI
PasI PshAI PspOMI SacI SapI SexAI SfcI SmaI
StuI StyI TaqII XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BclI BfrBI BglII BmgBI
BmrI BmtI BplI BpuEI BsaAI BsaBI BsiWI BsmI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BstBI BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI