Restriction map for
pFN21AE4061
(Linear) (Six-base) MAPSORT of: KIEE4061.seq Check: 869 from: 1 to: 1002
KIEE4061.orf
With 182 enzymes: SgfI *
March 22, 2012 17:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1002
Size: 289 713
AlwNI CAG_nnn'CTG
Cuts at: 0 594 1002
Size: 594 408
ApoI r'AATT_y
Cuts at: 0 52 73 427 649 847 892 913 1002
Size: 52 21 354 222 198 45 21 89
Fragments arranged by size:
354 222 198 89 52 45 21 21
AseI AT'TA_AT
Cuts at: 0 269 1002
Size: 269 733
BanII G_rGCy'C
Cuts at: 0 765 1002
Size: 765 237
BfrBI ATG'CAT
Cuts at: 0 843 1002
Size: 843 159
BlpI GC'TnA_GC
Cuts at: 0 584 1002
Size: 584 418
Bme1580I G_kGCm'C
Cuts at: 0 967 1002
Size: 967 35
BseYI C'CCAG_C
Cuts at: 0 291 1002
Size: 291 711
BsiHKAI G_wGCw'C
Cuts at: 0 765 1002
Size: 765 237
Bsp1286I G_dGCh'C
Cuts at: 0 765 967 1002
Size: 765 202 35
Fragments arranged by size:
765 202 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 579 1002
Size: 579 423
BsrGI T'GTAC_A
Cuts at: 0 542 1002
Size: 542 460
BstYI r'GATC_y
Cuts at: 0 116 1002
Size: 116 886
BtgI C'CryG_G
Cuts at: 0 878 1002
Size: 878 124
BtsI GCAGTG_nn'
Cuts at: 0 478 1002
Size: 478 524
EarI CTCTTCn'nnn_
Cuts at: 0 308 611 863 1002
Size: 308 303 252 139
Fragments arranged by size:
308 303 252 139
EcoICRI GAG'CTC
Cuts at: 0 763 1002
Size: 763 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1002
Size: 289 713
EcoO109I rG'GnC_Cy
Cuts at: 0 591 873 1002
Size: 591 282 129
Fragments arranged by size:
591 282 129
EcoRV GAT'ATC
Cuts at: 0 249 627 1002
Size: 249 378 375
Fragments arranged by size:
378 375 249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 427 1002
Size: 427 575
HaeII r_GCGC'y
Cuts at: 0 66 961 1002
Size: 66 895 41
Fragments arranged by size:
895 66 41
MfeI C'AATT_G
Cuts at: 0 692 710 1002
Size: 692 18 292
Fragments arranged by size:
692 292 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1002
Size: 826 176
MspA1I CmG'CkG
Cuts at: 0 291 758 939 1002
Size: 291 467 181 63
Fragments arranged by size:
467 291 181 63
NcoI C'CATG_G
Cuts at: 0 878 1002
Size: 878 124
NsiI A_TGCA'T
Cuts at: 0 845 1002
Size: 845 157
PpuMI rG'GwC_Cy
Cuts at: 0 591 873 1002
Size: 591 282 129
Fragments arranged by size:
591 282 129
PsiI TTA'TAA
Cuts at: 0 125 1002
Size: 125 877
PstI C_TGCA'G
Cuts at: 0 944 1002
Size: 944 58
PvuII CAG'CTG
Cuts at: 0 291 939 1002
Size: 291 648 63
Fragments arranged by size:
648 291 63
SacI G_AGCT'C
Cuts at: 0 765 1002
Size: 765 237
SfcI C'TryA_G
Cuts at: 0 239 940 1002
Size: 239 701 62
Fragments arranged by size:
701 239 62
StuI AGG'CCT
Cuts at: 0 376 1002
Size: 376 626
StyI C'CwwG_G
Cuts at: 0 492 878 1002
Size: 492 386 124
Fragments arranged by size:
492 386 124
TatI w'GTAC_w
Cuts at: 0 542 1002
Size: 542 460
XbaI T'CTAG_A
Cuts at: 0 145 1002
Size: 145 857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 447 885 1002
Size: 447 438 117
Fragments arranged by size:
447 438 117
XmnI GAAnn'nnTTC
Cuts at: 0 7 1002
Size: 7 995
Enzymes that do cut:
AcuI AlwNI ApoI AseI BanII BfrBI BlpI Bme1580I
BseYI BsiHKAI Bsp1286I BspMI BsrGI BstYI BtgI BtsI
EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII MfeI
MmeI MspA1I NcoI NsiI PpuMI PsiI PstI PvuII
SacI SfcI StuI StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI
EcoRI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MluI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XhoI XmaI ZraI