Restriction map for
pFN21AE4060
(Linear) (Six-base) MAPSORT of: KIEE4060.seq Check: 8173 from: 1 to: 1002
KIEE4060.orf
With 182 enzymes: SgfI *
March 22, 2012 17:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 595 1002
Size: 392 203 407
Fragments arranged by size:
407 392 203
AhdI GACnn_n'nnGTC
Cuts at: 0 138 299 1002
Size: 138 161 703
Fragments arranged by size:
703 161 138
AlwNI CAG_nnn'CTG
Cuts at: 0 156 850 1002
Size: 156 694 152
Fragments arranged by size:
694 156 152
ApaI G_GGCC'C
Cuts at: 0 13 1002
Size: 13 989
ApoI r'AATT_y
Cuts at: 0 478 993 1002
Size: 478 515 9
Fragments arranged by size:
515 478 9
BamHI G'GATC_C
Cuts at: 0 240 1002
Size: 240 762
BanII G_rGCy'C
Cuts at: 0 13 260 1002
Size: 13 247 742
Fragments arranged by size:
742 247 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 117 885 1002
Size: 117 768 117
Fragments arranged by size:
768 117 117
BfrBI ATG'CAT
Cuts at: 0 764 1002
Size: 764 238
BglI GCCn_nnn'nGGC
Cuts at: 0 48 60 1002
Size: 48 12 942
Fragments arranged by size:
942 48 12
Bme1580I G_kGCm'C
Cuts at: 0 13 1002
Size: 13 989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1002
Size: 178 824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 943 1002
Size: 751 192 59
Fragments arranged by size:
751 192 59
BsaBI GATnn'nnATC
Cuts at: 0 239 1002
Size: 239 763
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 157 166 187 1002
Size: 136 21 9 21 815
Fragments arranged by size:
815 136 21 21 9
BseYI C'CCAG_C
Cuts at: 0 57 201 1002
Size: 57 144 801
Fragments arranged by size:
801 144 57
BsiHKAI G_wGCw'C
Cuts at: 0 260 1002
Size: 260 742
BsmI GAATG_Cn'
Cuts at: 0 458 624 1002
Size: 458 166 378
Fragments arranged by size:
458 378 166
BsmBI CGTCTCn'nnnn_
Cuts at: 0 961 1002
Size: 961 41
Bsp1286I G_dGCh'C
Cuts at: 0 13 260 1002
Size: 13 247 742
Fragments arranged by size:
742 247 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 349 601 1002
Size: 236 113 252 401
Fragments arranged by size:
401 252 236 113
BsrDI GCAATG_nn'
Cuts at: 0 788 1002
Size: 788 214
BsrGI T'GTAC_A
Cuts at: 0 701 1002
Size: 701 301
BssHII G'CGCG_C
Cuts at: 0 92 1002
Size: 92 910
BstAPI GCAn_nnn'nTGC
Cuts at: 0 153 511 668 1002
Size: 153 358 157 334
Fragments arranged by size:
358 334 157 153
BstYI r'GATC_y
Cuts at: 0 234 240 1002
Size: 234 6 762
Fragments arranged by size:
762 234 6
BtgI C'CryG_G
Cuts at: 0 46 395 1002
Size: 46 349 607
Fragments arranged by size:
607 349 46
BtsI GCAGTG_nn'
Cuts at: 0 843 1002
Size: 843 159
EarI CTCTTCn'nnn_
Cuts at: 0 358 1002
Size: 358 644
EcoICRI GAG'CTC
Cuts at: 0 258 1002
Size: 258 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 392 595 1002
Size: 178 214 203 407
Fragments arranged by size:
407 214 203 178
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 1002
Size: 187 815
EcoO109I rG'GnC_Cy
Cuts at: 0 205 1002
Size: 205 797
FspI TGC'GCA
Cuts at: 0 310 1002
Size: 310 692
HaeII r_GCGC'y
Cuts at: 0 317 1002
Size: 317 685
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 157 189 285 317 1002
Size: 124 32 1 32 96 32 685
Fragments arranged by size:
685 124 96 32 32 32 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 381 1002
Size: 122 259 621
Fragments arranged by size:
621 259 122
MslI CAynn'nnrTG
Cuts at: 0 759 769 1002
Size: 759 10 233
Fragments arranged by size:
759 233 10
MspA1I CmG'CkG
Cuts at: 0 27 57 397 1002
Size: 27 30 340 605
Fragments arranged by size:
605 340 30 27
NsiI A_TGCA'T
Cuts at: 0 766 1002
Size: 766 236
PasI CC'CwG_GG
Cuts at: 0 87 1002
Size: 87 915
PspOMI G'GGCC_C
Cuts at: 0 9 1002
Size: 9 993
PstI C_TGCA'G
Cuts at: 0 191 1002
Size: 191 811
SacI G_AGCT'C
Cuts at: 0 260 1002
Size: 260 742
SacII CC_GC'GG
Cuts at: 0 398 1002
Size: 398 604
SbfI CC_TGCA'GG
Cuts at: 0 191 1002
Size: 191 811
SfcI C'TryA_G
Cuts at: 0 187 1002
Size: 187 815
SmlI C'TyrA_G
Cuts at: 0 730 958 1002
Size: 730 228 44
Fragments arranged by size:
730 228 44
SspI AAT'ATT
Cuts at: 0 984 1002
Size: 984 18
TatI w'GTAC_w
Cuts at: 0 701 1002
Size: 701 301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 1002
Size: 232 32 738
Fragments arranged by size:
738 232 32
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApoI BamHI BanII BbsI
BfrBI BglI Bme1580I BpmI BpuEI BsaBI BsaXI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BssHII
BstAPI BstYI BtgI BtsI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I MmeI MslI MspA1I NsiI
PasI PspOMI PstI SacI SacII SbfI SfcI SmlI
SspI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BclI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaHI BsaWI BseRI BsgI BsiEI BsiWI
BspEI BspHI BsrBI BsrFI BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI