Restriction map for
pFN21AE4059
(Linear) (Six-base) MAPSORT of: KIEE4059.seq Check: 8860 from: 1 to: 981
KIEE4059.orf
With 182 enzymes: SgfI *
March 22, 2012 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 95 187 933 981
Size: 95 92 746 48
Fragments arranged by size:
746 95 92 48
Acc65I G'GTAC_C
Cuts at: 0 114 981
Size: 114 867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 981
Size: 97 884
AfeI AGC'GCT
Cuts at: 0 650 981
Size: 650 331
AflIII A'CryG_T
Cuts at: 0 629 981
Size: 629 352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 812 981
Size: 208 604 169
Fragments arranged by size:
604 208 169
AlwNI CAG_nnn'CTG
Cuts at: 0 738 981
Size: 738 243
ApaI G_GGCC'C
Cuts at: 0 293 981
Size: 293 688
BanI G'GyrC_C
Cuts at: 0 114 282 573 981
Size: 114 168 291 408
Fragments arranged by size:
408 291 168 114
BanII G_rGCy'C
Cuts at: 0 293 981
Size: 293 688
BbeI G_GCGC'C
Cuts at: 0 286 577 981
Size: 286 291 404
Fragments arranged by size:
404 291 286
BbvCI CC'TCA_GC
Cuts at: 0 569 981
Size: 569 412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 614 648 981
Size: 614 34 333
Fragments arranged by size:
614 333 34
BclI T'GATC_A
Cuts at: 0 875 981
Size: 875 106
Bme1580I G_kGCm'C
Cuts at: 0 293 801 832 981
Size: 293 508 31 149
Fragments arranged by size:
508 293 149 31
BmgBI CAC'GTC
Cuts at: 0 632 981
Size: 632 349
BmrI ACTGGGnnnn_n'
Cuts at: 0 358 981
Size: 358 623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 559 981
Size: 91 468 422
Fragments arranged by size:
468 422 91
Bpu10I CC'TnA_GC
Cuts at: 0 569 844 981
Size: 569 275 137
Fragments arranged by size:
569 275 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 637 981
Size: 208 429 344
Fragments arranged by size:
429 344 208
BsaI GGTCTCn'nnnn_
Cuts at: 0 514 589 981
Size: 514 75 392
Fragments arranged by size:
514 392 75
BsaHI Gr'CG_yC
Cuts at: 0 283 574 981
Size: 283 291 407
Fragments arranged by size:
407 291 283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 313 556 981
Size: 79 234 243 425
Fragments arranged by size:
425 243 234 79
BseYI C'CCAG_C
Cuts at: 0 9 310 351 405 433 617 981
Size: 9 301 41 54 28 184 364
Fragments arranged by size:
364 301 184 54 41 28 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 808 981
Size: 546 262 173
Fragments arranged by size:
546 262 173
Bsp1286I G_dGCh'C
Cuts at: 0 293 801 832 981
Size: 293 508 31 149
Fragments arranged by size:
508 293 149 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 187 410 719 905 933 981
Size: 95 92 223 309 186 28 48
Fragments arranged by size:
309 223 186 95 92 48 28
BsrBI CCG'CTC
Cuts at: 0 42 776 981
Size: 42 734 205
Fragments arranged by size:
734 205 42
BsrDI GCAATG_nn'
Cuts at: 0 662 981
Size: 662 319
BstEII G'GTnAC_C
Cuts at: 0 495 702 981
Size: 495 207 279
Fragments arranged by size:
495 279 207
BstYI r'GATC_y
Cuts at: 0 446 509 981
Size: 446 63 472
Fragments arranged by size:
472 446 63
Bsu36I CC'TnA_GG
Cuts at: 0 197 981
Size: 197 784
BtgI C'CryG_G
Cuts at: 0 170 266 491 821 981
Size: 170 96 225 330 160
Fragments arranged by size:
330 225 170 160 96
BtsI GCAGTG_nn'
Cuts at: 0 342 981
Size: 342 639
DraIII CAC_nnn'GTG
Cuts at: 0 456 981
Size: 456 525
DrdI GACnn_nn'nnGTC
Cuts at: 0 634 981
Size: 634 347
EaeI y'GGCC_r
Cuts at: 0 2 122 173 473 981
Size: 2 120 51 300 508
Fragments arranged by size:
508 300 120 51 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 666 760 877 981
Size: 666 94 117 104
Fragments arranged by size:
666 117 104 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 97 559 981
Size: 91 6 462 422
Fragments arranged by size:
462 422 91 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 323 856 981
Size: 323 533 125
Fragments arranged by size:
533 323 125
EcoO109I rG'GnC_Cy
Cuts at: 0 289 316 861 981
Size: 289 27 545 120
Fragments arranged by size:
545 289 120 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 48 981
Size: 48 933
HaeII r_GCGC'y
Cuts at: 0 286 577 652 810 981
Size: 286 291 75 158 171
Fragments arranged by size:
291 286 171 158 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 513 545 981
Size: 513 32 436
Fragments arranged by size:
513 436 32
HincII GTy'rAC
Cuts at: 0 165 981
Size: 165 816
KasI G'GCGC_C
Cuts at: 0 282 573 981
Size: 282 291 408
Fragments arranged by size:
408 291 282
KpnI G_GTAC'C
Cuts at: 0 118 981
Size: 118 863
MscI TGG'CCA
Cuts at: 0 175 981
Size: 175 806
MspA1I CmG'CkG
Cuts at: 0 355 699 981
Size: 355 344 282
Fragments arranged by size:
355 344 282
NarI GG'CG_CC
Cuts at: 0 283 574 981
Size: 283 291 407
Fragments arranged by size:
407 291 283
NcoI C'CATG_G
Cuts at: 0 821 981
Size: 821 160
NspI r_CATG'y
Cuts at: 0 625 718 981
Size: 625 93 263
Fragments arranged by size:
625 263 93
PasI CC'CwG_GG
Cuts at: 0 937 981
Size: 937 44
PflMI CCAn_nnn'nTGG
Cuts at: 0 456 822 981
Size: 456 366 159
Fragments arranged by size:
456 366 159
PpuMI rG'GwC_Cy
Cuts at: 0 861 981
Size: 861 120
PshAI GACnn'nnGTC
Cuts at: 0 232 981
Size: 232 749
PspOMI G'GGCC_C
Cuts at: 0 289 981
Size: 289 692
PstI C_TGCA'G
Cuts at: 0 764 902 981
Size: 764 138 79
Fragments arranged by size:
764 138 79
PvuII CAG'CTG
Cuts at: 0 355 981
Size: 355 626
ScaI AGT'ACT
Cuts at: 0 559 981
Size: 559 422
SfcI C'TryA_G
Cuts at: 0 356 760 898 981
Size: 356 404 138 83
Fragments arranged by size:
404 356 138 83
SfoI GGC'GCC
Cuts at: 0 284 575 981
Size: 284 291 406
Fragments arranged by size:
406 291 284
SmlI C'TyrA_G
Cuts at: 0 223 652 981
Size: 223 429 329
Fragments arranged by size:
429 329 223
SphI G_CATG'C
Cuts at: 0 625 981
Size: 625 356
StuI AGG'CCT
Cuts at: 0 793 981
Size: 793 188
StyI C'CwwG_G
Cuts at: 0 821 981
Size: 821 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 624 981
Size: 244 380 357
Fragments arranged by size:
380 357 244
TatI w'GTAC_w
Cuts at: 0 557 981
Size: 557 424
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 315 347 981
Size: 315 32 634
Fragments arranged by size:
634 315 32
XmnI GAAnn'nnTTC
Cuts at: 0 100 981
Size: 100 881
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlfI AlwNI ApaI
BanI BanII BbeI BbvCI BcgI BclI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI
BsgI Bsp1286I BspMI BsrBI BsrDI BstEII BstYI Bsu36I
BtgI BtsI DraIII DrdI EaeI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI KpnI MscI
MspA1I NarI NcoI NspI PasI PflMI PpuMI PshAI
PspOMI PstI PvuII ScaI SfcI SfoI SmlI SphI
StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbsI BciVI BfrBI BglI BglII BlpI BmtI
BplI BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI
EagI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI MfeI MluI MmeI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI