Restriction map for
pFN21AE4056
(Linear) (Six-base) MAPSORT of: KIEE4056.seq Check: 2351 from: 1 to: 930
KIEE4056.orf
With 182 enzymes: SgfI *
March 22, 2012 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 930
Size: 282 648
AatII G_ACGT'C
Cuts at: 0 711 930
Size: 711 219
AccI GT'mk_AC
Cuts at: 0 509 930
Size: 509 421
AflIII A'CryG_T
Cuts at: 0 446 930
Size: 446 484
ApaI G_GGCC'C
Cuts at: 0 384 925 930
Size: 384 541 5
Fragments arranged by size:
541 384 5
AvaI C'yCGr_G
Cuts at: 0 383 433 577 930
Size: 383 50 144 353
Fragments arranged by size:
383 353 144 50
AvrII C'CTAG_G
Cuts at: 0 631 855 930
Size: 631 224 75
Fragments arranged by size:
631 224 75
BanI G'GyrC_C
Cuts at: 0 270 930
Size: 270 660
BanII G_rGCy'C
Cuts at: 0 384 427 434 440 617 925 930
Size: 384 43 7 6 177 308 5
Fragments arranged by size:
384 308 177 43 7 6 5
Bme1580I G_kGCm'C
Cuts at: 0 19 292 384 925 930
Size: 19 273 92 541 5
Fragments arranged by size:
541 273 92 19 5
BmgBI CAC'GTC
Cuts at: 0 449 930
Size: 449 481
BmrI ACTGGGnnnn_n'
Cuts at: 0 234 387 403 930
Size: 234 153 16 527
Fragments arranged by size:
527 234 153 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 930
Size: 769 161
Bpu10I CC'TnA_GC
Cuts at: 0 611 742 930
Size: 611 131 188
Fragments arranged by size:
611 188 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 764 930
Size: 764 166
BsaHI Gr'CG_yC
Cuts at: 0 708 930
Size: 708 222
BsaWI w'CCGG_w
Cuts at: 0 662 930
Size: 662 268
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 383 413 659 689 930
Size: 383 30 246 30 241
Fragments arranged by size:
383 246 241 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 930
Size: 517 413
BseYI C'CCAG_C
Cuts at: 0 251 292 528 558 754 819 930
Size: 251 41 236 30 196 65 111
Fragments arranged by size:
251 236 196 111 65 41 30
BsiEI CG_ry'CG
Cuts at: 0 715 772 930
Size: 715 57 158
Fragments arranged by size:
715 158 57
BsiHKAI G_wGCw'C
Cuts at: 0 77 930
Size: 77 853
BsmBI CGTCTCn'nnnn_
Cuts at: 0 184 930
Size: 184 746
Bsp1286I G_dGCh'C
Cuts at: 0 19 77 292 384 427 434 440 617
Size: 19 58 215 92 43 7 6 177
Cuts at: 617 925 930
Size: 308 5
Fragments arranged by size:
308 215 177 92 58 43 19 7
6 5
BspHI T'CATG_A
Cuts at: 0 899 930
Size: 899 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 282 930
Size: 128 154 648
Fragments arranged by size:
648 154 128
BsrDI GCAATG_nn'
Cuts at: 0 130 306 930
Size: 130 176 624
Fragments arranged by size:
624 176 130
BsrFI r'CCGG_y
Cuts at: 0 277 572 714 930
Size: 277 295 142 216
Fragments arranged by size:
295 277 216 142
BstBI TT'CG_AA
Cuts at: 0 50 930
Size: 50 880
BstEII G'GTnAC_C
Cuts at: 0 30 930
Size: 30 900
BtgI C'CryG_G
Cuts at: 0 338 678 930
Size: 338 340 252
Fragments arranged by size:
340 338 252
BtsI GCAGTG_nn'
Cuts at: 0 75 930
Size: 75 855
ClaI AT'CG_AT
Cuts at: 0 410 930
Size: 410 520
DraIII CAC_nnn'GTG
Cuts at: 0 591 930
Size: 591 339
EaeI y'GGCC_r
Cuts at: 0 179 365 712 769 930
Size: 179 186 347 57 161
Fragments arranged by size:
347 186 179 161 57
EagI C'GGCC_G
Cuts at: 0 712 769 930
Size: 712 57 161
Fragments arranged by size:
712 161 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 930
Size: 769 161
EcoO109I rG'GnC_Cy
Cuts at: 0 546 922 930
Size: 546 376 8
Fragments arranged by size:
546 376 8
FseI GG_CCGG'CC
Cuts at: 0 576 718 930
Size: 576 142 212
Fragments arranged by size:
576 212 142
HaeII r_GCGC'y
Cuts at: 0 518 930
Size: 518 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 502 534 930
Size: 383 32 87 32 396
Fragments arranged by size:
396 383 87 32 32
HincII GTy'rAC
Cuts at: 0 63 375 930
Size: 63 312 555
Fragments arranged by size:
555 312 63
MfeI C'AATT_G
Cuts at: 0 153 930
Size: 153 777
MscI TGG'CCA
Cuts at: 0 181 367 930
Size: 181 186 563
Fragments arranged by size:
563 186 181
MslI CAynn'nnrTG
Cuts at: 0 916 930
Size: 916 14
MspA1I CmG'CkG
Cuts at: 0 296 774 789 871 930
Size: 296 478 15 82 59
Fragments arranged by size:
478 296 82 59 15
NaeI GCC'GGC
Cuts at: 0 279 574 716 930
Size: 279 295 142 214
Fragments arranged by size:
295 279 214 142
NcoI C'CATG_G
Cuts at: 0 678 930
Size: 678 252
NgoMIV G'CCGG_C
Cuts at: 0 277 572 714 930
Size: 277 295 142 216
Fragments arranged by size:
295 277 216 142
NotI GC'GGCC_GC
Cuts at: 0 769 930
Size: 769 161
PspOMI G'GGCC_C
Cuts at: 0 380 921 930
Size: 380 541 9
Fragments arranged by size:
541 380 9
PvuII CAG'CTG
Cuts at: 0 296 871 930
Size: 296 575 59
Fragments arranged by size:
575 296 59
SfcI C'TryA_G
Cuts at: 0 537 930
Size: 537 393
SmaI CCC'GGG
Cuts at: 0 385 930
Size: 385 545
SrfI GCCC'GGGC
Cuts at: 0 385 930
Size: 385 545
StuI AGG'CCT
Cuts at: 0 674 930
Size: 674 256
StyI C'CwwG_G
Cuts at: 0 631 678 719 855 930
Size: 631 47 41 136 75
Fragments arranged by size:
631 136 75 47 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 659 691 930
Size: 659 32 239
Fragments arranged by size:
659 239 32
XmaI C'CCGG_G
Cuts at: 0 383 930
Size: 383 547
ZraI GAC'GTC
Cuts at: 0 709 930
Size: 709 221
Enzymes that do cut:
AarI AatII AccI AflIII ApaI AvaI AvrII BanI
BanII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BstBI BstEII BtgI BtsI
ClaI DraIII EaeI EagI Eco57MI EcoO109I FseI HaeII
Hin4I HincII MfeI MscI MslI MspA1I NaeI NcoI
NgoMIV NotI PspOMI PvuII SfcI SmaI SrfI StuI
StyI TstI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI BpuEI BsaAI
BsaBI BsgI BsiWI BsmI BspEI BsrBI BsrGI BssHII
BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgZI CspCI
DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FspI FspAI HindIII HpaI KasI KpnI MluI
MmeI NarI NdeI NheI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI