(Linear) (Six-base) MAPSORT of: KIEE4054.seq  Check: 4262  from: 1  to: 915



KIEE4054.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     915 
   Size:        254     661
 
AflIII A'CryG_T

Cuts at:      0     880     915 
   Size:        880      35
 
ApoI r'AATT_y

Cuts at:      0     485     915 
   Size:        485     430
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     770     915 
   Size:        770     145
 
BclI T'GATC_A

Cuts at:      0     380     915 
   Size:        380     535
 
BfrBI ATG'CAT

Cuts at:      0     851     915 
   Size:        851      64
 
Bme1580I G_kGCm'C

Cuts at:      0     653     915 
   Size:        653     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     651     915 
   Size:        651     264
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     500     530     660     690     915 
   Size:        500      30     130      30     225

  Fragments arranged by size: 

                 500     225     130      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     188     915 
   Size:        188     727
 
BsmI GAATG_Cn'

Cuts at:      0     255     548     915 
   Size:        255     293     367

  Fragments arranged by size: 

                 367     293     255
 
Bsp1286I G_dGCh'C

Cuts at:      0     653     915 
   Size:        653     262
 
BtsI GCAGTG_nn'

Cuts at:      0     559     915 
   Size:        559     356
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     651     915 
   Size:        254     397     264

  Fragments arranged by size: 

                 397     264     254
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174     660     692     915 
   Size:        142      32     486      32     223

  Fragments arranged by size: 

                 486     223     142      32      32
 
MfeI C'AATT_G

Cuts at:      0     602     915 
   Size:        602     313
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     915 
   Size:        123     792
 
MslI CAynn'nnrTG

Cuts at:      0     532     915 
   Size:        532     383
 
NsiI A_TGCA'T

Cuts at:      0     853     915 
   Size:        853      62
 
NspI r_CATG'y

Cuts at:      0     552     851     884     915 
   Size:        552     299      33      31

  Fragments arranged by size: 

                 552     299      33      31
 
PciI A'CATG_T

Cuts at:      0     880     915 
   Size:        880      35
 
SphI G_CATG'C

Cuts at:      0     851     915 
   Size:        851      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     546     915 
   Size:        546     369
 
TatI w'GTAC_w

Cuts at:      0     328     915 
   Size:        328     587
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     500     532     915 
   Size:        500      32     383

  Fragments arranged by size: 

                 500     383      32
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BbsI     BclI    BfrBI Bme1580I     BpmI
    BsaXI    BseRI     BsmI Bsp1286I     BtsI  Eco57MI    Hin4I     MfeI
     MmeI     MslI     NsiI     NspI     PciI     SphI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI