(Linear) (Six-base) MAPSORT of: KIEE4050.seq  Check: 6167  from: 1  to: 879



KIEE4050.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:15  ..

AccI GT'mk_AC

Cuts at:      0     702     879 
   Size:        702     177
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480     676     879 
   Size:        480     196     203

  Fragments arranged by size: 

                 480     203     196
 
AflIII A'CryG_T

Cuts at:      0     189     879 
   Size:        189     690
 
ApaLI G'TGCA_C

Cuts at:      0     119     879 
   Size:        119     760
 
ApoI r'AATT_y

Cuts at:      0     144     751     879 
   Size:        144     607     128

  Fragments arranged by size: 

                 607     144     128
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      98     131     879 
   Size:         98      33     748

  Fragments arranged by size: 

                 748      98      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      22      56     879 
   Size:         22      34     823

  Fragments arranged by size: 

                 823      34      22
 
BfrBI ATG'CAT

Cuts at:      0     281     879 
   Size:        281     598
 
Bme1580I G_kGCm'C

Cuts at:      0      35     123     879 
   Size:         35      88     756

  Fragments arranged by size: 

                 756      88      35
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      86     879 
   Size:         86     793
 
BsiHKAI G_wGCw'C

Cuts at:      0     123     879 
   Size:        123     756
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     123     879 
   Size:         35      88     756

  Fragments arranged by size: 

                 756      88      35
 
BspHI T'CATG_A

Cuts at:      0     392     822     879 
   Size:        392     430      57

  Fragments arranged by size: 

                 430     392      57
 
BstZ17I GTA'TAC

Cuts at:      0     703     879 
   Size:        703     176
 
BtsI GCAGTG_nn'

Cuts at:      0     457     879 
   Size:        457     422
 
DraI TTT'AAA

Cuts at:      0     273     872     879 
   Size:        273     599       7

  Fragments arranged by size: 

                 599     273       7
 
EarI CTCTTCn'nnn_

Cuts at:      0     377     652     879 
   Size:        377     275     227

  Fragments arranged by size: 

                 377     275     227
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480     676     879 
   Size:        480     196     203

  Fragments arranged by size: 

                 480     203     196
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     309     510     879 
   Size:        309     201     369

  Fragments arranged by size: 

                 369     309     201
 
HindIII A'AGCT_T

Cuts at:      0     397     665     879 
   Size:        397     268     214

  Fragments arranged by size: 

                 397     268     214
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     600     879 
   Size:        600     279
 
MslI CAynn'nnrTG

Cuts at:      0     286     879 
   Size:        286     593
 
MspA1I CmG'CkG

Cuts at:      0     321     879 
   Size:        321     558
 
NdeI CA'TA_TG

Cuts at:      0     357     879 
   Size:        357     522
 
NsiI A_TGCA'T

Cuts at:      0     283     879 
   Size:        283     596
 
NspI r_CATG'y

Cuts at:      0     193     879 
   Size:        193     686
 
PciI A'CATG_T

Cuts at:      0     189     879 
   Size:        189     690
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     452     879 
   Size:        452     427
 
PsiI TTA'TAA

Cuts at:      0     314     879 
   Size:        314     565
 
PstI C_TGCA'G

Cuts at:      0     302     879 
   Size:        302     577
 
PvuII CAG'CTG

Cuts at:      0     321     879 
   Size:        321     558
 
SapI GCTCTTCn'nnn_

Cuts at:      0     377     879 
   Size:        377     502
 
SfcI C'TryA_G

Cuts at:      0     298     373     879 
   Size:        298      75     506

  Fragments arranged by size: 

                 506     298      75
 
SpeI A'CTAG_T

Cuts at:      0     553     879 
   Size:        553     326
 
SspI AAT'ATT

Cuts at:      0     101     246     879 
   Size:        101     145     633

  Fragments arranged by size: 

                 633     145     101
 
XmnI GAAnn'nnTTC

Cuts at:      0     719     879 
   Size:        719     160
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    ApaLI     ApoI     BaeI     BcgI    BfrBI
 Bme1580I     BmrI  BsiHKAI Bsp1286I    BspHI  BstZ17I     BtsI     DraI
     EarI  Eco57MI     FalI  HindIII     MmeI     MslI   MspA1I     NdeI
     NsiI     NspI     PciI    PflMI     PsiI     PstI    PvuII     SapI
     SfcI     SpeI     SspI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI