Restriction map for
pFN21AE4049
(Linear) (Six-base) MAPSORT of: KIEE4049.seq Check: 4243 from: 1 to: 834
KIEE4049.orf
With 182 enzymes: SgfI *
March 22, 2012 17:15 ..
AatII G_ACGT'C
Cuts at: 0 416 834
Size: 416 418
AfeI AGC'GCT
Cuts at: 0 678 834
Size: 678 156
AflIII A'CryG_T
Cuts at: 0 662 834
Size: 662 172
AlwNI CAG_nnn'CTG
Cuts at: 0 210 834
Size: 210 624
ApaI G_GGCC'C
Cuts at: 0 228 834
Size: 228 606
ApaLI G'TGCA_C
Cuts at: 0 67 834
Size: 67 767
AvaI C'yCGr_G
Cuts at: 0 8 282 476 629 834
Size: 8 274 194 153 205
Fragments arranged by size:
274 205 194 153 8
BanI G'GyrC_C
Cuts at: 0 124 834
Size: 124 710
BanII G_rGCy'C
Cuts at: 0 8 228 834
Size: 8 220 606
Fragments arranged by size:
606 220 8
BbeI G_GCGC'C
Cuts at: 0 128 834
Size: 128 706
Bme1580I G_kGCm'C
Cuts at: 0 71 228 477 834
Size: 71 157 249 357
Fragments arranged by size:
357 249 157 71
BmgBI CAC'GTC
Cuts at: 0 39 834
Size: 39 795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 834
Size: 796 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 834
Size: 768 66
BsaAI yAC'GTr
Cuts at: 0 665 834
Size: 665 169
BsaBI GATnn'nnATC
Cuts at: 0 601 834
Size: 601 233
BsaHI Gr'CG_yC
Cuts at: 0 125 413 764 834
Size: 125 288 351 70
Fragments arranged by size:
351 288 125 70
BseYI C'CCAG_C
Cuts at: 0 80 220 834
Size: 80 140 614
Fragments arranged by size:
614 140 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 223 346 445 834
Size: 151 72 123 99 389
Fragments arranged by size:
389 151 123 99 72
BsiHKAI G_wGCw'C
Cuts at: 0 71 834
Size: 71 763
BsmI GAATG_Cn'
Cuts at: 0 534 834
Size: 534 300
Bsp1286I G_dGCh'C
Cuts at: 0 8 71 228 477 834
Size: 8 63 157 249 357
Fragments arranged by size:
357 249 157 63 8
BspHI T'CATG_A
Cuts at: 0 543 834
Size: 543 291
BsrBI CCG'CTC
Cuts at: 0 57 122 264 380 834
Size: 57 65 142 116 454
Fragments arranged by size:
454 142 116 65 57
BsrGI T'GTAC_A
Cuts at: 0 344 834
Size: 344 490
BssSI C'ACGA_G
Cuts at: 0 70 364 834
Size: 70 294 470
Fragments arranged by size:
470 294 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 210 834
Size: 210 624
BstEII G'GTnAC_C
Cuts at: 0 141 834
Size: 141 693
BtgI C'CryG_G
Cuts at: 0 146 639 834
Size: 146 493 195
Fragments arranged by size:
493 195 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 284 834
Size: 284 550
EaeI y'GGCC_r
Cuts at: 0 566 834
Size: 566 268
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 834
Size: 31 803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 834
Size: 796 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 364 834
Size: 364 470
EcoO109I rG'GnC_Cy
Cuts at: 0 224 452 834
Size: 224 228 382
Fragments arranged by size:
382 228 224
HaeII r_GCGC'y
Cuts at: 0 128 680 834
Size: 128 552 154
Fragments arranged by size:
552 154 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 808 834
Size: 808 26
HincII GTy'rAC
Cuts at: 0 333 585 834
Size: 333 252 249
Fragments arranged by size:
333 252 249
HindIII A'AGCT_T
Cuts at: 0 538 834
Size: 538 296
KasI G'GCGC_C
Cuts at: 0 124 834
Size: 124 710
MslI CAynn'nnrTG
Cuts at: 0 595 834
Size: 595 239
MspA1I CmG'CkG
Cuts at: 0 84 102 111 207 641 834
Size: 84 18 9 96 434 193
Fragments arranged by size:
434 193 96 84 18 9
NarI GG'CG_CC
Cuts at: 0 125 834
Size: 125 709
PmlI CAC'GTG
Cuts at: 0 665 834
Size: 665 169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 834
Size: 369 32 433
Fragments arranged by size:
433 369 32
PpuMI rG'GwC_Cy
Cuts at: 0 452 834
Size: 452 382
PspOMI G'GGCC_C
Cuts at: 0 224 834
Size: 224 610
PstI C_TGCA'G
Cuts at: 0 101 185 739 834
Size: 101 84 554 95
Fragments arranged by size:
554 101 95 84
PvuII CAG'CTG
Cuts at: 0 102 111 207 834
Size: 102 9 96 627
Fragments arranged by size:
627 102 96 9
SacII CC_GC'GG
Cuts at: 0 642 834
Size: 642 192
SanDI GG'GwC_CC
Cuts at: 0 452 834
Size: 452 382
ScaI AGT'ACT
Cuts at: 0 700 834
Size: 700 134
SexAI A'CCwGG_T
Cuts at: 0 137 834
Size: 137 697
SfcI C'TryA_G
Cuts at: 0 97 181 735 834
Size: 97 84 554 99
Fragments arranged by size:
554 99 97 84
SfoI GGC'GCC
Cuts at: 0 126 834
Size: 126 708
SmaI CCC'GGG
Cuts at: 0 10 478 631 834
Size: 10 468 153 203
Fragments arranged by size:
468 203 153 10
SmlI C'TyrA_G
Cuts at: 0 747 834
Size: 747 87
StuI AGG'CCT
Cuts at: 0 370 834
Size: 370 464
StyI C'CwwG_G
Cuts at: 0 326 569 834
Size: 326 243 265
Fragments arranged by size:
326 265 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 834
Size: 39 795
TatI w'GTAC_w
Cuts at: 0 344 698 834
Size: 344 354 136
Fragments arranged by size:
354 344 136
Tth111I GACn'n_nGTC
Cuts at: 0 493 723 834
Size: 493 230 111
Fragments arranged by size:
493 230 111
XmaI C'CCGG_G
Cuts at: 0 8 476 629 834
Size: 8 468 153 205
Fragments arranged by size:
468 205 153 8
ZraI GAC'GTC
Cuts at: 0 414 834
Size: 414 420
Enzymes that do cut:
AatII AfeI AflIII AlwNI ApaI ApaLI AvaI BanI
BanII BbeI Bme1580I BmgBI BpmI BpuEI BsaAI BsaBI
BsaHI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrGI BssSI BstAPI BstEII BtgI BtgZI EaeI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI
MslI MspA1I NarI PmlI PpiI PpuMI PspOMI PstI
PvuII SacII SanDI ScaI SexAI SfcI SfoI SmaI
SmlI StuI StyI TaqII TatI Tth111I XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaI
BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI BspMI
BsrDI BsrFI BssHII BstBI BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI
KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SapI SbfI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XcmI XhoI XmnI