(Linear) (Six-base) MAPSORT of: KIEE4046.seq  Check: 5648  from: 1  to: 819



KIEE4046.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:14  ..

AatII G_ACGT'C

Cuts at:      0     450     819 
   Size:        450     369
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     819 
   Size:        473     346
 
AflIII A'CryG_T

Cuts at:      0     174     819 
   Size:        174     645
 
ApoI r'AATT_y

Cuts at:      0     632     819 
   Size:        632     187
 
AseI AT'TA_AT

Cuts at:      0     512     819 
   Size:        512     307
 
BmgBI CAC'GTC

Cuts at:      0     177     819 
   Size:        177     642
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     819 
   Size:        521     298
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     669     819 
   Size:        669     150
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      44     819 
   Size:         44     775
 
BsaHI Gr'CG_yC

Cuts at:      0     314     447     819 
   Size:        314     133     372

  Fragments arranged by size: 

                 372     314     133
 
BseYI C'CCAG_C

Cuts at:      0     663     819 
   Size:        663     156
 
BsmI GAATG_Cn'

Cuts at:      0     792     819 
   Size:        792      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      26     819 
   Size:         26     793
 
BstBI TT'CG_AA

Cuts at:      0     243     636     819 
   Size:        243     393     183

  Fragments arranged by size: 

                 393     243     183
 
BtgI C'CryG_G

Cuts at:      0      62     819 
   Size:         62     757
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     406     819 
   Size:        406     413
 
BtsI GCAGTG_nn'

Cuts at:      0     597     765     819 
   Size:        597     168      54

  Fragments arranged by size: 

                 597     168      54
 
DraIII CAC_nnn'GTG

Cuts at:      0     271     819 
   Size:        271     548
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     160     819 
   Size:        160     659
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     521     819 
   Size:        473      48     298

  Fragments arranged by size: 

                 473     298      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     680     819 
   Size:        680     139
 
EcoRI G'AATT_C

Cuts at:      0     632     819 
   Size:        632     187
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     466     819 
   Size:        466     353
 
HincII GTy'rAC

Cuts at:      0     201     819 
   Size:        201     618
 
HpaI GTT'AAC

Cuts at:      0     201     819 
   Size:        201     618
 
MfeI C'AATT_G

Cuts at:      0     143     819 
   Size:        143     676
 
MslI CAynn'nnrTG

Cuts at:      0      61     425     819 
   Size:         61     364     394

  Fragments arranged by size: 

                 394     364      61
 
NcoI C'CATG_G

Cuts at:      0      62     819 
   Size:         62     757
 
NspI r_CATG'y

Cuts at:      0      15     663     819 
   Size:         15     648     156

  Fragments arranged by size: 

                 648     156      15
 
PsiI TTA'TAA

Cuts at:      0      97     819 
   Size:         97     722
 
SmlI C'TyrA_G

Cuts at:      0     684     819 
   Size:        684     135
 
SspI AAT'ATT

Cuts at:      0     378     819 
   Size:        378     441
 
StyI C'CwwG_G

Cuts at:      0      62     819 
   Size:         62     757
 
TatI w'GTAC_w

Cuts at:      0     476     819 
   Size:        476     343
 
XmnI GAAnn'nnTTC

Cuts at:      0     118     819 
   Size:        118     701
 
ZraI GAC'GTC

Cuts at:      0     448     819 
   Size:        448     371
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     ApoI     AseI    BmgBI     BpmI    BpuEI
     BsaI    BsaHI    BseYI     BsmI    BspMI    BstBI     BtgI    BtgZI
     BtsI   DraIII     DrdI  Eco57MI EcoO109I    EcoRI     FalI   HincII
     HpaI     MfeI     MslI     NcoI     NspI     PsiI     SmlI     SspI
     StyI     TatI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     KasI     KpnI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI