Restriction map for
pFN21AE4044
(Linear) (Six-base) MAPSORT of: KIEE4044.seq Check: 821 from: 1 to: 801
KIEE4044.orf
With 182 enzymes: SgfI *
March 22, 2012 17:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 801
Size: 601 200
AfeI AGC'GCT
Cuts at: 0 612 801
Size: 612 189
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 682 714 801
Size: 682 32 87
Fragments arranged by size:
682 87 32
AlwNI CAG_nnn'CTG
Cuts at: 0 54 801
Size: 54 747
ApaI G_GGCC'C
Cuts at: 0 126 234 440 801
Size: 126 108 206 361
Fragments arranged by size:
361 206 126 108
AvaI C'yCGr_G
Cuts at: 0 76 233 801
Size: 76 157 568
Fragments arranged by size:
568 157 76
BanI G'GyrC_C
Cuts at: 0 131 486 550 801
Size: 131 355 64 251
Fragments arranged by size:
355 251 131 64
BanII G_rGCy'C
Cuts at: 0 126 234 440 447 801
Size: 126 108 206 7 354
Fragments arranged by size:
354 206 126 108 7
BbeI G_GCGC'C
Cuts at: 0 135 801
Size: 135 666
BciVI GTATCCnnnnn_n'
Cuts at: 0 464 801
Size: 464 337
Bme1580I G_kGCm'C
Cuts at: 0 126 234 304 440 553 801
Size: 126 108 70 136 113 248
Fragments arranged by size:
248 136 126 113 108 70
BmgBI CAC'GTC
Cuts at: 0 682 801
Size: 682 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 444 801
Size: 444 357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 801
Size: 502 299
BsaHI Gr'CG_yC
Cuts at: 0 132 801
Size: 132 669
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 535 801
Size: 535 266
BseYI C'CCAG_C
Cuts at: 0 125 369 395 790 801
Size: 125 244 26 395 11
Fragments arranged by size:
395 244 125 26 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 247 801
Size: 86 161 554
Fragments arranged by size:
554 161 86
BsiHKAI G_wGCw'C
Cuts at: 0 223 801
Size: 223 578
BsmI GAATG_Cn'
Cuts at: 0 568 801
Size: 568 233
Bsp1286I G_dGCh'C
Cuts at: 0 126 223 234 304 440 447 553 801
Size: 126 97 11 70 136 7 106 248
Fragments arranged by size:
248 136 126 106 97 70 11 7
BsrBI CCG'CTC
Cuts at: 0 201 801
Size: 201 600
BtgI C'CryG_G
Cuts at: 0 135 209 311 326 573 801
Size: 135 74 102 15 247 228
Fragments arranged by size:
247 228 135 102 74 15
BtsI GCAGTG_nn'
Cuts at: 0 716 801
Size: 716 85
EaeI y'GGCC_r
Cuts at: 0 323 801
Size: 323 478
EarI CTCTTCn'nnn_
Cuts at: 0 83 735 801
Size: 83 652 66
Fragments arranged by size:
652 83 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 601 801
Size: 502 99 200
Fragments arranged by size:
502 200 99
EcoO109I rG'GnC_Cy
Cuts at: 0 51 122 230 258 436 590 801
Size: 51 71 108 28 178 154 211
Fragments arranged by size:
211 178 154 108 71 51 28
HaeII r_GCGC'y
Cuts at: 0 135 367 614 801
Size: 135 232 247 187
Fragments arranged by size:
247 232 187 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 582 614 712 744 801
Size: 82 32 468 32 98 32 57
Fragments arranged by size:
468 98 82 57 32 32 32
KasI G'GCGC_C
Cuts at: 0 131 801
Size: 131 670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 801
Size: 121 680
MscI TGG'CCA
Cuts at: 0 325 801
Size: 325 476
MspA1I CmG'CkG
Cuts at: 0 129 281 342 460 480 575 801
Size: 129 152 61 118 20 95 226
Fragments arranged by size:
226 152 129 118 95 61 20
NarI GG'CG_CC
Cuts at: 0 132 801
Size: 132 669
NspI r_CATG'y
Cuts at: 0 310 801
Size: 310 491
PpuMI rG'GwC_Cy
Cuts at: 0 590 801
Size: 590 211
PspOMI G'GGCC_C
Cuts at: 0 122 230 436 801
Size: 122 108 206 365
Fragments arranged by size:
365 206 122 108
PstI C_TGCA'G
Cuts at: 0 347 801
Size: 347 454
PvuII CAG'CTG
Cuts at: 0 281 480 801
Size: 281 199 321
Fragments arranged by size:
321 281 199
SacII CC_GC'GG
Cuts at: 0 576 801
Size: 576 225
SapI GCTCTTCn'nnn_
Cuts at: 0 83 801
Size: 83 718
SfcI C'TryA_G
Cuts at: 0 343 801
Size: 343 458
SfoI GGC'GCC
Cuts at: 0 133 801
Size: 133 668
SmaI CCC'GGG
Cuts at: 0 235 801
Size: 235 566
SphI G_CATG'C
Cuts at: 0 310 801
Size: 310 491
StuI AGG'CCT
Cuts at: 0 670 801
Size: 670 131
TatI w'GTAC_w
Cuts at: 0 191 801
Size: 191 610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 223 801
Size: 223 578
XmaI C'CCGG_G
Cuts at: 0 233 801
Size: 233 568
XmnI GAAnn'nnTTC
Cuts at: 0 729 801
Size: 729 72
Enzymes that do cut:
AcuI AfeI AloI AlwNI ApaI AvaI BanI BanII
BbeI BciVI Bme1580I BmgBI BmrI BpmI BsaHI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BtgI BtsI
EaeI EarI Eco57MI EcoO109I HaeII Hin4I KasI MmeI
MscI MspA1I NarI NspI PpuMI PspOMI PstI PvuII
SacII SapI SfcI SfoI SmaI SphI StuI TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StyI SwaI TaqII TstI Tth111I XbaI XhoI
ZraI