Restriction map for
pFN21AE4043
(Linear) (Six-base) MAPSORT of: KIEE4043.seq Check: 7040 from: 1 to: 798
KIEE4043.orf
With 182 enzymes: SgfI *
March 22, 2012 17:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 432 798
Size: 432 366
AfeI AGC'GCT
Cuts at: 0 103 798
Size: 103 695
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 760 792 798
Size: 478 32 250 32 6
Fragments arranged by size:
478 250 32 32 6
AlwNI CAG_nnn'CTG
Cuts at: 0 63 447 798
Size: 63 384 351
Fragments arranged by size:
384 351 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 717 798
Size: 365 352 81
Fragments arranged by size:
365 352 81
BclI T'GATC_A
Cuts at: 0 399 798
Size: 399 399
BglII A'GATC_T
Cuts at: 0 566 798
Size: 566 232
BmgBI CAC'GTC
Cuts at: 0 96 798
Size: 96 702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 541 798
Size: 40 501 257
Fragments arranged by size:
501 257 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 234 798
Size: 234 564
BsaHI Gr'CG_yC
Cuts at: 0 26 798
Size: 26 772
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 733 763 798
Size: 184 30 519 30 35
Fragments arranged by size:
519 184 35 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 233 580 798
Size: 233 347 218
Fragments arranged by size:
347 233 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 798
Size: 487 311
BsiEI CG_ry'CG
Cuts at: 0 510 798
Size: 510 288
BsmI GAATG_Cn'
Cuts at: 0 672 798
Size: 672 126
BsmBI CGTCTCn'nnnn_
Cuts at: 0 538 798
Size: 538 260
BspHI T'CATG_A
Cuts at: 0 29 798
Size: 29 769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 432 798
Size: 432 366
BsrDI GCAATG_nn'
Cuts at: 0 144 798
Size: 144 654
BsrFI r'CCGG_y
Cuts at: 0 44 361 798
Size: 44 317 437
Fragments arranged by size:
437 317 44
BssSI C'ACGA_G
Cuts at: 0 709 798
Size: 709 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 447 798
Size: 447 351
BstYI r'GATC_y
Cuts at: 0 566 713 798
Size: 566 147 85
Fragments arranged by size:
566 147 85
BtgI C'CryG_G
Cuts at: 0 72 798
Size: 72 726
BtsI GCAGTG_nn'
Cuts at: 0 114 687 798
Size: 114 573 111
Fragments arranged by size:
573 114 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 451 486 798
Size: 451 35 312
Fragments arranged by size:
451 312 35
EaeI y'GGCC_r
Cuts at: 0 507 647 798
Size: 507 140 151
Fragments arranged by size:
507 151 140
EagI C'GGCC_G
Cuts at: 0 507 798
Size: 507 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 541 798
Size: 40 501 257
Fragments arranged by size:
501 257 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 595 798
Size: 595 203
HaeII r_GCGC'y
Cuts at: 0 51 105 798
Size: 51 54 693
Fragments arranged by size:
693 54 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 733 765 798
Size: 733 32 33
Fragments arranged by size:
733 33 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 798
Size: 137 661
MscI TGG'CCA
Cuts at: 0 649 798
Size: 649 149
NcoI C'CATG_G
Cuts at: 0 72 798
Size: 72 726
PfoI T'CCnGG_A
Cuts at: 0 518 539 798
Size: 518 21 259
Fragments arranged by size:
518 259 21
PshAI GACnn'nnGTC
Cuts at: 0 37 798
Size: 37 761
PstI C_TGCA'G
Cuts at: 0 452 798
Size: 452 346
ScaI AGT'ACT
Cuts at: 0 655 798
Size: 655 143
SexAI A'CCwGG_T
Cuts at: 0 392 798
Size: 392 406
SfcI C'TryA_G
Cuts at: 0 448 798
Size: 448 350
SgrAI Cr'CCGG_yG
Cuts at: 0 44 798
Size: 44 754
StyI C'CwwG_G
Cuts at: 0 72 798
Size: 72 726
TatI w'GTAC_w
Cuts at: 0 653 798
Size: 653 145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 522 798
Size: 252 270 276
Fragments arranged by size:
276 270 252
Enzymes that do cut:
AarI AfeI AloI AlwNI BbsI BclI BglII BmgBI
BpmI BsaI BsaHI BsaXI BseRI BsgI BsiEI BsmI
BsmBI BspHI BspMI BsrDI BsrFI BssSI BstAPI BstYI
BtgI BtsI CspCI EaeI EagI Eco57MI EcoNI HaeII
Hin4I MmeI MscI NcoI PfoI PshAI PstI ScaI
SexAI SfcI SgrAI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BseYI
BsiHKAI BsiWI Bsp1286I BspEI BsrBI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII
DrdI EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI