(Linear) (Six-base) MAPSORT of: KIEE4042.seq  Check: 5672  from: 1  to: 651



KIEE4042.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     195     651 
   Size:        195     456
 
AccI GT'mk_AC

Cuts at:      0     122     651 
   Size:        122     529
 
AfeI AGC'GCT

Cuts at:      0     232     651 
   Size:        232     419
 
AflIII A'CryG_T

Cuts at:      0      91     651 
   Size:         91     560
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     568     651 
   Size:        568      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      94     504     651 
   Size:         94     410     147

  Fragments arranged by size: 

                 410     147      94
 
BlpI GC'TnA_GC

Cuts at:      0      15     651 
   Size:         15     636
 
Bme1580I G_kGCm'C

Cuts at:      0     270     576     651 
   Size:        270     306      75

  Fragments arranged by size: 

                 306     270      75
 
BmgBI CAC'GTC

Cuts at:      0      92     417     651 
   Size:         92     325     234

  Fragments arranged by size: 

                 325     234      92
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     582     651 
   Size:        137     445      69

  Fragments arranged by size: 

                 445     137      69
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     420     538     627     651 
   Size:        121     299     118      89      24

  Fragments arranged by size: 

                 299     121     118      89      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     521     651 
   Size:        521     130
 
Bsp1286I G_dGCh'C

Cuts at:      0     270     521     576     651 
   Size:        270     251      55      75

  Fragments arranged by size: 

                 270     251      75      55
 
BspHI T'CATG_A

Cuts at:      0     336     651 
   Size:        336     315
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     195     651 
   Size:        195     456
 
BssHII G'CGCG_C

Cuts at:      0     541     651 
   Size:        541     110
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     267     651 
   Size:        267     384
 
BtsI GCAGTG_nn'

Cuts at:      0     270     639     651 
   Size:        270     369      12

  Fragments arranged by size: 

                 369     270      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     628     651 
   Size:        628      23
 
EaeI y'GGCC_r

Cuts at:      0     222     464     524     651 
   Size:        222     242      60     127

  Fragments arranged by size: 

                 242     222     127      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     582     651 
   Size:        137     445      69

  Fragments arranged by size: 

                 445     137      69
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     424     651 
   Size:        424     227
 
HaeII r_GCGC'y

Cuts at:      0     234     651 
   Size:        234     417
 
MscI TGG'CCA

Cuts at:      0     224     526     651 
   Size:        224     302     125

  Fragments arranged by size: 

                 302     224     125
 
MslI CAynn'nnrTG

Cuts at:      0     560     651 
   Size:        560      91
 
MspA1I CmG'CkG

Cuts at:      0     220     651 
   Size:        220     431
 
PasI CC'CwG_GG

Cuts at:      0     618     651 
   Size:        618      33
 
PvuII CAG'CTG

Cuts at:      0     220     651 
   Size:        220     431
 
StyI C'CwwG_G

Cuts at:      0     485     527     651 
   Size:        485      42     124

  Fragments arranged by size: 

                 485     124      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     262     651 
   Size:        262     389
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      78     110     651 
   Size:         78      32     541

  Fragments arranged by size: 

                 541      78      32
 

 Enzymes that do cut:

     AarI     AccI     AfeI   AflIII     AlfI     BbsI     BlpI Bme1580I
    BmgBI     BpmI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI   BssHII
    BtgZI     BtsI    CspCI     EaeI  Eco57MI    EcoNI    HaeII     MscI
     MslI   MspA1I     PasI    PvuII     StyI    TaqII     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI