Restriction map for
pFN21AE4039
(Linear) (Six-base) MAPSORT of: KIEE4039.seq Check: 5424 from: 1 to: 774
KIEE4039.orf
With 182 enzymes: SgfI *
March 22, 2012 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 228 774
Size: 228 546
AccI GT'mk_AC
Cuts at: 0 387 774
Size: 387 387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 774
Size: 683 91
AflIII A'CryG_T
Cuts at: 0 251 774
Size: 251 523
ApoI r'AATT_y
Cuts at: 0 677 774
Size: 677 97
AseI AT'TA_AT
Cuts at: 0 467 774
Size: 467 307
BanI G'GyrC_C
Cuts at: 0 322 774
Size: 322 452
BclI T'GATC_A
Cuts at: 0 429 774
Size: 429 345
BfrBI ATG'CAT
Cuts at: 0 550 774
Size: 550 224
BglII A'GATC_T
Cuts at: 0 502 774
Size: 502 272
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 774
Size: 289 485
BsaWI w'CCGG_w
Cuts at: 0 479 774
Size: 479 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 774
Size: 265 30 479
Fragments arranged by size:
479 265 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 774
Size: 256 518
BsmI GAATG_Cn'
Cuts at: 0 550 774
Size: 550 224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 228 774
Size: 228 546
BsrGI T'GTAC_A
Cuts at: 0 141 774
Size: 141 633
BstAPI GCAn_nnn'nTGC
Cuts at: 0 223 774
Size: 223 551
BstEII G'GTnAC_C
Cuts at: 0 396 774
Size: 396 378
BstYI r'GATC_y
Cuts at: 0 502 525 774
Size: 502 23 249
Fragments arranged by size:
502 249 23
EaeI y'GGCC_r
Cuts at: 0 2 774
Size: 2 772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 774
Size: 683 91
EcoO109I rG'GnC_Cy
Cuts at: 0 576 774
Size: 576 198
EcoRI G'AATT_C
Cuts at: 0 677 774
Size: 677 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 441 450 473 774
Size: 418 23 9 23 301
Fragments arranged by size:
418 301 23 23 9
MscI TGG'CCA
Cuts at: 0 4 774
Size: 4 770
MslI CAynn'nnrTG
Cuts at: 0 250 774
Size: 250 524
NsiI A_TGCA'T
Cuts at: 0 552 774
Size: 552 222
NspI r_CATG'y
Cuts at: 0 255 550 774
Size: 255 295 224
Fragments arranged by size:
295 255 224
PciI A'CATG_T
Cuts at: 0 251 774
Size: 251 523
PsiI TTA'TAA
Cuts at: 0 476 774
Size: 476 298
ScaI AGT'ACT
Cuts at: 0 77 774
Size: 77 697
SfcI C'TryA_G
Cuts at: 0 384 774
Size: 384 390
SphI G_CATG'C
Cuts at: 0 550 774
Size: 550 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 173 774
Size: 173 601
TatI w'GTAC_w
Cuts at: 0 75 141 254 774
Size: 75 66 113 520
Fragments arranged by size:
520 113 75 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 774
Size: 265 32 477
Fragments arranged by size:
477 265 32
XbaI T'CTAG_A
Cuts at: 0 648 690 774
Size: 648 42 84
Fragments arranged by size:
648 84 42
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 507 774
Size: 507 267
XmnI GAAnn'nnTTC
Cuts at: 0 197 774
Size: 197 577
Enzymes that do cut:
AarI AccI AcuI AflIII ApoI AseI BanI BclI
BfrBI BglII BsaI BsaWI BsaXI BsgI BsmI BspMI
BsrGI BstAPI BstEII BstYI EaeI Eco57MI EcoO109I EcoRI
Hin4I MscI MslI NsiI NspI PciI PsiI ScaI
SfcI SphI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI BseYI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstBI BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
Tth111I XhoI XmaI ZraI