Restriction map for
pFN21AE4034
(Linear) (Six-base) MAPSORT of: KIEE4034.seq Check: 3457 from: 1 to: 732
KIEE4034.orf
With 182 enzymes: SgfI *
July 3, 2012 18:25 ..
AccI GT'mk_AC
Cuts at: 0 609 732
Size: 609 123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 732
Size: 295 437
AlwNI CAG_nnn'CTG
Cuts at: 0 402 501 564 732
Size: 402 99 63 168
Fragments arranged by size:
402 168 99 63
ApaI G_GGCC'C
Cuts at: 0 22 492 732
Size: 22 470 240
Fragments arranged by size:
470 240 22
ApaLI G'TGCA_C
Cuts at: 0 697 732
Size: 697 35
AvaI C'yCGr_G
Cuts at: 0 163 184 732
Size: 163 21 548
Fragments arranged by size:
548 163 21
BanI G'GyrC_C
Cuts at: 0 3 43 691 732
Size: 3 40 648 41
Fragments arranged by size:
648 41 40 3
BanII G_rGCy'C
Cuts at: 0 22 64 492 725 732
Size: 22 42 428 233 7
Fragments arranged by size:
428 233 42 22 7
BbeI G_GCGC'C
Cuts at: 0 7 47 732
Size: 7 40 685
Fragments arranged by size:
685 40 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 91 732
Size: 91 641
Bme1580I G_kGCm'C
Cuts at: 0 22 492 701 732
Size: 22 470 209 31
Fragments arranged by size:
470 209 31 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 732
Size: 235 497
Bpu10I CC'TnA_GC
Cuts at: 0 565 732
Size: 565 167
BsaHI Gr'CG_yC
Cuts at: 0 4 44 732
Size: 4 40 688
Fragments arranged by size:
688 40 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 217 232 715 732
Size: 208 9 15 483 17
Fragments arranged by size:
483 208 17 15 9
BseYI C'CCAG_C
Cuts at: 0 15 36 57 116 246 603 732
Size: 15 21 21 59 130 357 129
Fragments arranged by size:
357 130 129 59 21 21 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 732
Size: 487 245
BsiEI CG_ry'CG
Cuts at: 0 13 99 105 732
Size: 13 86 6 627
Fragments arranged by size:
627 86 13 6
BsiHKAI G_wGCw'C
Cuts at: 0 71 274 701 725 732
Size: 71 203 427 24 7
Fragments arranged by size:
427 203 71 24 7
Bsp1286I G_dGCh'C
Cuts at: 0 22 64 71 274 492 701 725 732
Size: 22 42 7 203 218 209 24 7
Fragments arranged by size:
218 209 203 42 24 22 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 410 539 732
Size: 383 27 129 193
Fragments arranged by size:
383 193 129 27
BsrBI CCG'CTC
Cuts at: 0 111 732
Size: 111 621
BsrDI GCAATG_nn'
Cuts at: 0 656 732
Size: 656 76
BsrFI r'CCGG_y
Cuts at: 0 207 732
Size: 207 525
BstAPI GCAn_nnn'nTGC
Cuts at: 0 510 732
Size: 510 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 582 732
Size: 582 150
BtgI C'CryG_G
Cuts at: 0 7 47 99 732
Size: 7 40 52 633
Fragments arranged by size:
633 52 40 7
ClaI AT'CG_AT
Cuts at: 0 620 732
Size: 620 112
DrdI GACnn_nn'nnGTC
Cuts at: 0 535 732
Size: 535 197
EaeI y'GGCC_r
Cuts at: 0 10 96 102 548 732
Size: 10 86 6 446 184
Fragments arranged by size:
446 184 86 10 6
EagI C'GGCC_G
Cuts at: 0 10 96 102 732
Size: 10 86 6 630
Fragments arranged by size:
630 86 10 6
EarI CTCTTCn'nnn_
Cuts at: 0 76 677 732
Size: 76 601 55
Fragments arranged by size:
601 76 55
EcoICRI GAG'CTC
Cuts at: 0 723 732
Size: 723 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 732
Size: 235 497
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 732
Size: 568 164
EcoO109I rG'GnC_Cy
Cuts at: 0 19 180 522 561 670 732
Size: 19 161 342 39 109 62
Fragments arranged by size:
342 161 109 62 39 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 108 732
Size: 108 624
HaeII r_GCGC'y
Cuts at: 0 7 37 47 117 214 732
Size: 7 30 10 70 97 518
Fragments arranged by size:
518 97 70 30 10 7
KasI G'GCGC_C
Cuts at: 0 3 43 732
Size: 3 40 689
Fragments arranged by size:
689 40 3
MscI TGG'CCA
Cuts at: 0 550 732
Size: 550 182
MslI CAynn'nnrTG
Cuts at: 0 580 732
Size: 580 152
MspA1I CmG'CkG
Cuts at: 0 9 15 49 101 306 597 630 732
Size: 9 6 34 52 205 291 33 102
Fragments arranged by size:
291 205 102 52 34 33 9 6
NaeI GCC'GGC
Cuts at: 0 209 732
Size: 209 523
NarI GG'CG_CC
Cuts at: 0 4 44 732
Size: 4 40 688
Fragments arranged by size:
688 40 4
NgoMIV G'CCGG_C
Cuts at: 0 207 732
Size: 207 525
NotI GC'GGCC_GC
Cuts at: 0 10 732
Size: 10 722
PasI CC'CwG_GG
Cuts at: 0 485 732
Size: 485 247
PpuMI rG'GwC_Cy
Cuts at: 0 180 522 561 670 732
Size: 180 342 39 109 62
Fragments arranged by size:
342 180 109 62 39
PspOMI G'GGCC_C
Cuts at: 0 18 488 732
Size: 18 470 244
Fragments arranged by size:
470 244 18
PstI C_TGCA'G
Cuts at: 0 596 669 732
Size: 596 73 63
Fragments arranged by size:
596 73 63
SacI G_AGCT'C
Cuts at: 0 725 732
Size: 725 7
SacII CC_GC'GG
Cuts at: 0 10 50 102 732
Size: 10 40 52 630
Fragments arranged by size:
630 52 40 10
SanDI GG'GwC_CC
Cuts at: 0 180 732
Size: 180 552
SapI GCTCTTCn'nnn_
Cuts at: 0 76 677 732
Size: 76 601 55
Fragments arranged by size:
601 76 55
SbfI CC_TGCA'GG
Cuts at: 0 669 732
Size: 669 63
SfcI C'TryA_G
Cuts at: 0 592 610 665 732
Size: 592 18 55 67
Fragments arranged by size:
592 67 55 18
SfoI GGC'GCC
Cuts at: 0 5 45 732
Size: 5 40 687
Fragments arranged by size:
687 40 5
StuI AGG'CCT
Cuts at: 0 133 732
Size: 133 599
StyI C'CwwG_G
Cuts at: 0 23 356 732
Size: 23 333 376
Fragments arranged by size:
376 333 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 485 732
Size: 485 247
Enzymes that do cut:
AccI AlfI AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BbsI Bme1580I BpmI Bpu10I BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstAPI BstXI BtgI ClaI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII KasI MscI
MslI MspA1I NaeI NarI NgoMIV NotI PasI PpuMI
PspOMI PstI SacI SacII SanDI SapI SbfI SfcI
SfoI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII EciI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SalI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI