Restriction map for
pFN21AE4032
(Linear) (Six-base) MAPSORT of: KIEE4032.seq Check: 3928 from: 1 to: 726
KIEE4032.orf
With 182 enzymes: SgfI *
July 3, 2012 18:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 704 726
Size: 285 419 22
Fragments arranged by size:
419 285 22
AflIII A'CryG_T
Cuts at: 0 458 726
Size: 458 268
ApaI G_GGCC'C
Cuts at: 0 282 726
Size: 282 444
ApaLI G'TGCA_C
Cuts at: 0 349 726
Size: 349 377
ApoI r'AATT_y
Cuts at: 0 493 726
Size: 493 233
AvaI C'yCGr_G
Cuts at: 0 54 122 726
Size: 54 68 604
Fragments arranged by size:
604 68 54
BanI G'GyrC_C
Cuts at: 0 4 88 156 190 726
Size: 4 84 68 34 536
Fragments arranged by size:
536 84 68 34 4
BanII G_rGCy'C
Cuts at: 0 61 282 406 726
Size: 61 221 124 320
Fragments arranged by size:
320 221 124 61
BbeI G_GCGC'C
Cuts at: 0 8 92 194 726
Size: 8 84 102 532
Fragments arranged by size:
532 102 84 8
BciVI GTATCCnnnnn_n'
Cuts at: 0 510 726
Size: 510 216
BfrBI ATG'CAT
Cuts at: 0 656 726
Size: 656 70
BglI GCCn_nnn'nGGC
Cuts at: 0 199 208 226 286 332 726
Size: 199 9 18 60 46 394
Fragments arranged by size:
394 199 60 46 18 9
BglII A'GATC_T
Cuts at: 0 700 726
Size: 700 26
Bme1580I G_kGCm'C
Cuts at: 0 161 282 353 481 726
Size: 161 121 71 128 245
Fragments arranged by size:
245 161 128 121 71
Bpu10I CC'TnA_GC
Cuts at: 0 152 710 726
Size: 152 558 16
Fragments arranged by size:
558 152 16
BsaBI GATnn'nnATC
Cuts at: 0 521 726
Size: 521 205
BsaHI Gr'CG_yC
Cuts at: 0 5 89 191 271 726
Size: 5 84 102 80 455
Fragments arranged by size:
455 102 84 80 5
BseYI C'CCAG_C
Cuts at: 0 160 175 586 726
Size: 160 15 411 140
Fragments arranged by size:
411 160 140 15
BsiEI CG_ry'CG
Cuts at: 0 26 39 203 381 391 726
Size: 26 13 164 178 10 335
Fragments arranged by size:
335 178 164 26 13 10
BsiHKAI G_wGCw'C
Cuts at: 0 353 716 726
Size: 353 363 10
Fragments arranged by size:
363 353 10
BsiWI C'GTAC_G
Cuts at: 0 329 726
Size: 329 397
Bsp1286I G_dGCh'C
Cuts at: 0 61 161 282 353 406 481 716 726
Size: 61 100 121 71 53 75 235 10
Fragments arranged by size:
235 121 100 75 71 61 53 10
BsrBI CCG'CTC
Cuts at: 0 17 242 726
Size: 17 225 484
Fragments arranged by size:
484 225 17
BsrFI r'CCGG_y
Cuts at: 0 386 390 726
Size: 386 4 336
Fragments arranged by size:
386 336 4
BssHII G'CGCG_C
Cuts at: 0 45 164 726
Size: 45 119 562
Fragments arranged by size:
562 119 45
BstYI r'GATC_y
Cuts at: 0 63 700 726
Size: 63 637 26
Fragments arranged by size:
637 63 26
Bsu36I CC'TnA_GG
Cuts at: 0 362 726
Size: 362 364
BtgI C'CryG_G
Cuts at: 0 101 134 194 203 257 726
Size: 101 33 60 9 54 469
Fragments arranged by size:
469 101 60 54 33 9
DrdI GACnn_nn'nnGTC
Cuts at: 0 647 726
Size: 647 79
EaeI y'GGCC_r
Cuts at: 0 23 36 200 378 388 726
Size: 23 13 164 178 10 338
Fragments arranged by size:
338 178 164 23 13 10
EagI C'GGCC_G
Cuts at: 0 23 36 200 378 388 726
Size: 23 13 164 178 10 338
Fragments arranged by size:
338 178 164 23 13 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 704 726
Size: 285 419 22
Fragments arranged by size:
419 285 22
EcoO109I rG'GnC_Cy
Cuts at: 0 278 726
Size: 278 448
HaeII r_GCGC'y
Cuts at: 0 8 92 122 182 194 726
Size: 8 84 30 60 12 532
Fragments arranged by size:
532 84 60 30 12 8
KasI G'GCGC_C
Cuts at: 0 4 88 190 726
Size: 4 84 102 536
Fragments arranged by size:
536 102 84 4
MspA1I CmG'CkG
Cuts at: 0 83 103 136 196 205 323 726
Size: 83 20 33 60 9 118 403
Fragments arranged by size:
403 118 83 60 33 20 9
NarI GG'CG_CC
Cuts at: 0 5 89 191 726
Size: 5 84 102 535
Fragments arranged by size:
535 102 84 5
NotI GC'GGCC_GC
Cuts at: 0 200 726
Size: 200 526
NsiI A_TGCA'T
Cuts at: 0 658 726
Size: 658 68
NspI r_CATG'y
Cuts at: 0 462 726
Size: 462 264
PciI A'CATG_T
Cuts at: 0 458 726
Size: 458 268
PspOMI G'GGCC_C
Cuts at: 0 278 726
Size: 278 448
SacII CC_GC'GG
Cuts at: 0 104 137 197 206 726
Size: 104 33 60 9 520
Fragments arranged by size:
520 104 60 33 9
SfcI C'TryA_G
Cuts at: 0 632 726
Size: 632 94
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 208 726
Size: 208 518
SfoI GGC'GCC
Cuts at: 0 6 90 192 726
Size: 6 84 102 534
Fragments arranged by size:
534 102 84 6
TatI w'GTAC_w
Cuts at: 0 570 726
Size: 570 156
Enzymes that do cut:
AcuI AflIII ApaI ApaLI ApoI AvaI BanI BanII
BbeI BciVI BfrBI BglI BglII Bme1580I Bpu10I BsaBI
BsaHI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BsrBI BsrFI
BssHII BstYI Bsu36I BtgI DrdI EaeI EagI Eco57MI
EcoO109I HaeII KasI MspA1I NarI NotI NsiI NspI
PciI PspOMI SacII SfcI SfiI SfoI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BclI BlpI
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BsgI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI NaeI NcoI NdeI NgoMIV NheI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI