Restriction map for
pFN21AE4031
(Linear) (Six-base) MAPSORT of: KIEE4031.seq Check: 337 from: 1 to: 726
KIEE4031.orf
With 182 enzymes: SgfI *
March 22, 2012 17:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 55 160 169 450 726
Size: 55 105 9 281 276
Fragments arranged by size:
281 276 105 55 9
Acc65I G'GTAC_C
Cuts at: 0 65 243 726
Size: 65 178 483
Fragments arranged by size:
483 178 65
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 574 726
Size: 526 48 152
Fragments arranged by size:
526 152 48
AhdI GACnn_n'nnGTC
Cuts at: 0 95 726
Size: 95 631
AleI CACnn'nnGTG
Cuts at: 0 85 726
Size: 85 641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 511 726
Size: 325 186 215
Fragments arranged by size:
325 215 186
AlwNI CAG_nnn'CTG
Cuts at: 0 119 726
Size: 119 607
BamHI G'GATC_C
Cuts at: 0 518 726
Size: 518 208
BanI G'GyrC_C
Cuts at: 0 65 243 309 684 726
Size: 65 178 66 375 42
Fragments arranged by size:
375 178 66 65 42
BbeI G_GCGC'C
Cuts at: 0 688 726
Size: 688 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 528 562 726
Size: 528 34 164
Fragments arranged by size:
528 164 34
BlpI GC'TnA_GC
Cuts at: 0 528 726
Size: 528 198
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 354 726
Size: 354 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 496 726
Size: 157 339 230
Fragments arranged by size:
339 230 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 726
Size: 631 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 726
Size: 325 401
BsaBI GATnn'nnATC
Cuts at: 0 172 362 726
Size: 172 190 364
Fragments arranged by size:
364 190 172
BsaHI Gr'CG_yC
Cuts at: 0 77 685 726
Size: 77 608 41
Fragments arranged by size:
608 77 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 236 266 726
Size: 236 30 460
Fragments arranged by size:
460 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 430 442 634 726
Size: 154 276 12 192 92
Fragments arranged by size:
276 192 154 92 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 85 682 726
Size: 78 7 597 44
Fragments arranged by size:
597 78 44 7
BsiEI CG_ry'CG
Cuts at: 0 542 726
Size: 542 184
BspHI T'CATG_A
Cuts at: 0 207 726
Size: 207 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 55 160 169 343 450 726
Size: 55 105 9 174 107 276
Fragments arranged by size:
276 174 107 105 55 9
BsrBI CCG'CTC
Cuts at: 0 690 726
Size: 690 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 119 726
Size: 119 607
BstEII G'GTnAC_C
Cuts at: 0 87 726
Size: 87 639
BstXI CCAn_nnnn'nTGG
Cuts at: 0 370 726
Size: 370 356
BstYI r'GATC_y
Cuts at: 0 518 623 726
Size: 518 105 103
Fragments arranged by size:
518 105 103
BtgI C'CryG_G
Cuts at: 0 83 410 726
Size: 83 327 316
Fragments arranged by size:
327 316 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 549 726
Size: 549 177
DraIII CAC_nnn'GTG
Cuts at: 0 668 726
Size: 668 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 496 526 574 726
Size: 157 339 30 48 152
Fragments arranged by size:
339 157 152 48 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 111 631 726
Size: 111 520 95
Fragments arranged by size:
520 111 95
HaeII r_GCGC'y
Cuts at: 0 688 726
Size: 688 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 81 113 726
Size: 81 32 613
Fragments arranged by size:
613 81 32
HincII GTy'rAC
Cuts at: 0 5 726
Size: 5 721
KasI G'GCGC_C
Cuts at: 0 684 726
Size: 684 42
KpnI G_GTAC'C
Cuts at: 0 69 247 726
Size: 69 178 479
Fragments arranged by size:
479 178 69
MslI CAynn'nnrTG
Cuts at: 0 85 307 368 726
Size: 85 222 61 358
Fragments arranged by size:
358 222 85 61
MspA1I CmG'CkG
Cuts at: 0 62 412 524 726
Size: 62 350 112 202
Fragments arranged by size:
350 202 112 62
NarI GG'CG_CC
Cuts at: 0 685 726
Size: 685 41
PstI C_TGCA'G
Cuts at: 0 115 166 197 726
Size: 115 51 31 529
Fragments arranged by size:
529 115 51 31
SacII CC_GC'GG
Cuts at: 0 413 726
Size: 413 313
SbfI CC_TGCA'GG
Cuts at: 0 166 726
Size: 166 560
SfcI C'TryA_G
Cuts at: 0 111 162 193 702 726
Size: 111 51 31 509 24
Fragments arranged by size:
509 111 51 31 24
SfoI GGC'GCC
Cuts at: 0 686 726
Size: 686 40
SmlI C'TyrA_G
Cuts at: 0 646 726
Size: 646 80
StuI AGG'CCT
Cuts at: 0 251 726
Size: 251 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 193 726
Size: 106 87 533
Fragments arranged by size:
533 106 87
TatI w'GTAC_w
Cuts at: 0 197 726
Size: 197 529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 726
Size: 343 32 351
Fragments arranged by size:
351 343 32
XmnI GAAnn'nnTTC
Cuts at: 0 559 726
Size: 559 167
Enzymes that do cut:
AarI Acc65I AcuI AhdI AleI AlfI AlwNI BamHI
BanI BbeI BcgI BlpI BplI BpmI BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BsgI BsiEI BspHI BspMI
BsrBI BstAPI BstEII BstXI BstYI BtgI BtgZI DraIII
Eco57MI EcoNI HaeII Hin4I HincII KasI KpnI MslI
MspA1I NarI PstI SacII SbfI SfcI SfoI SmlI
StuI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BanII BbsI BbvCI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI BsaWI
BseYI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI MfeI MluI MmeI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI