Restriction map for
pFN21AE4030
(Linear) (Six-base) MAPSORT of: KIEE4030.seq Check: 9147 from: 1 to: 711
KIEE4030.orf
With 182 enzymes: SgfI *
March 22, 2012 17:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 103 177 617 711
Size: 103 74 440 94
Fragments arranged by size:
440 103 94 74
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 711
Size: 373 338
AflIII A'CryG_T
Cuts at: 0 152 711
Size: 152 559
AleI CACnn'nnGTG
Cuts at: 0 256 643 711
Size: 256 387 68
Fragments arranged by size:
387 256 68
AlwNI CAG_nnn'CTG
Cuts at: 0 492 609 711
Size: 492 117 102
Fragments arranged by size:
492 117 102
ApaLI G'TGCA_C
Cuts at: 0 334 711
Size: 334 377
BanI G'GyrC_C
Cuts at: 0 189 399 466 711
Size: 189 210 67 245
Fragments arranged by size:
245 210 189 67
BglI GCCn_nnn'nGGC
Cuts at: 0 54 58 679 711
Size: 54 4 621 32
Fragments arranged by size:
621 54 32 4
BlpI GC'TnA_GC
Cuts at: 0 32 711
Size: 32 679
Bme1580I G_kGCm'C
Cuts at: 0 208 338 404 471 547 711
Size: 208 130 66 67 76 164
Fragments arranged by size:
208 164 130 76 67 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 130 157 711
Size: 100 30 27 554
Fragments arranged by size:
554 100 30 27
BsaAI yAC'GTr
Cuts at: 0 258 711
Size: 258 453
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 613 643 711
Size: 73 30 510 30 68
Fragments arranged by size:
510 73 68 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 679 711
Size: 679 32
BseYI C'CCAG_C
Cuts at: 0 421 577 711
Size: 421 156 134
Fragments arranged by size:
421 156 134
BsiEI CG_ry'CG
Cuts at: 0 53 711
Size: 53 658
BsiHKAI G_wGCw'C
Cuts at: 0 338 711
Size: 338 373
Bsp1286I G_dGCh'C
Cuts at: 0 208 338 404 471 547 711
Size: 208 130 66 67 76 164
Fragments arranged by size:
208 164 130 76 67 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 177 617 711
Size: 103 74 440 94
Fragments arranged by size:
440 103 94 74
BsrDI GCAATG_nn'
Cuts at: 0 566 711
Size: 566 145
BsrFI r'CCGG_y
Cuts at: 0 48 57 122 711
Size: 48 9 65 589
Fragments arranged by size:
589 65 48 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 609 711
Size: 609 102
BstEII G'GTnAC_C
Cuts at: 0 312 711
Size: 312 399
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 240 258 537 711
Size: 108 132 18 279 174
Fragments arranged by size:
279 174 132 108 18
BtgI C'CryG_G
Cuts at: 0 128 395 711
Size: 128 267 316
Fragments arranged by size:
316 267 128
DraIII CAC_nnn'GTG
Cuts at: 0 507 711
Size: 507 204
EaeI y'GGCC_r
Cuts at: 0 50 131 147 371 711
Size: 50 81 16 224 340
Fragments arranged by size:
340 224 81 50 16
EagI C'GGCC_G
Cuts at: 0 50 711
Size: 50 661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 130 157 373 711
Size: 100 30 27 216 338
Fragments arranged by size:
338 216 100 30 27
EcoO109I rG'GnC_Cy
Cuts at: 0 573 711
Size: 573 138
HaeII r_GCGC'y
Cuts at: 0 289 593 711
Size: 289 304 118
Fragments arranged by size:
304 289 118
MscI TGG'CCA
Cuts at: 0 133 373 711
Size: 133 240 338
Fragments arranged by size:
338 240 133
MslI CAynn'nnrTG
Cuts at: 0 256 643 711
Size: 256 387 68
Fragments arranged by size:
387 256 68
MspA1I CmG'CkG
Cuts at: 0 39 74 143 201 711
Size: 39 35 69 58 510
Fragments arranged by size:
510 69 58 39 35
NaeI GCC'GGC
Cuts at: 0 50 59 711
Size: 50 9 652
Fragments arranged by size:
652 50 9
NgoMIV G'CCGG_C
Cuts at: 0 48 57 711
Size: 48 9 654
Fragments arranged by size:
654 48 9
PasI CC'CwG_GG
Cuts at: 0 566 711
Size: 566 145
PfoI T'CCnGG_A
Cuts at: 0 359 711
Size: 359 352
PmlI CAC'GTG
Cuts at: 0 258 711
Size: 258 453
PpuMI rG'GwC_Cy
Cuts at: 0 573 711
Size: 573 138
PstI C_TGCA'G
Cuts at: 0 188 605 711
Size: 188 417 106
Fragments arranged by size:
417 188 106
PvuII CAG'CTG
Cuts at: 0 143 711
Size: 143 568
SanDI GG'GwC_CC
Cuts at: 0 573 711
Size: 573 138
SbfI CC_TGCA'GG
Cuts at: 0 188 711
Size: 188 523
SfcI C'TryA_G
Cuts at: 0 184 601 651 711
Size: 184 417 50 60
Fragments arranged by size:
417 184 60 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 711
Size: 268 443
TatI w'GTAC_w
Cuts at: 0 89 711
Size: 89 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 328 360 401 433 613 645 711
Size: 328 32 41 32 180 32 66
Fragments arranged by size:
328 180 66 41 32 32 32
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaLI BanI BglI
BlpI Bme1580I BpmI BsaAI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI BstEII BstXI
BtgI DraIII EaeI EagI Eco57MI EcoO109I HaeII MscI
MslI MspA1I NaeI NgoMIV PasI PfoI PmlI PpuMI
PstI PvuII SanDI SbfI SfcI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI