Restriction map for
pFN21AE4027
(Linear) (Six-base) MAPSORT of: KIEE4027.seq Check: 6584 from: 1 to: 708
KIEE4027.orf
With 182 enzymes: SgfI *
March 22, 2012 17:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 662 708
Size: 548 114 46
Fragments arranged by size:
548 114 46
AfeI AGC'GCT
Cuts at: 0 465 708
Size: 465 243
AhdI GACnn_n'nnGTC
Cuts at: 0 619 708
Size: 619 89
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 708
Size: 220 488
ApoI r'AATT_y
Cuts at: 0 404 708
Size: 404 304
BanII G_rGCy'C
Cuts at: 0 270 708
Size: 270 438
BciVI GTATCCnnnnn_n'
Cuts at: 0 197 708
Size: 197 511
BglI GCCn_nnn'nGGC
Cuts at: 0 540 708
Size: 540 168
BglII A'GATC_T
Cuts at: 0 453 708
Size: 453 255
BmtI G_CTAG'C
Cuts at: 0 139 708
Size: 139 569
Bpu10I CC'TnA_GC
Cuts at: 0 538 708
Size: 538 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 457 708
Size: 280 177 251
Fragments arranged by size:
280 251 177
BsaHI Gr'CG_yC
Cuts at: 0 543 708
Size: 543 165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 257 287 708
Size: 257 30 421
Fragments arranged by size:
421 257 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 143 568 708
Size: 143 425 140
Fragments arranged by size:
425 143 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 708
Size: 687 21
BsiHKAI G_wGCw'C
Cuts at: 0 270 708
Size: 270 438
Bsp1286I G_dGCh'C
Cuts at: 0 270 708
Size: 270 438
BsrDI GCAATG_nn'
Cuts at: 0 505 708
Size: 505 203
BstYI r'GATC_y
Cuts at: 0 159 453 708
Size: 159 294 255
Fragments arranged by size:
294 255 159
EarI CTCTTCn'nnn_
Cuts at: 0 206 524 708
Size: 206 318 184
Fragments arranged by size:
318 206 184
EcoICRI GAG'CTC
Cuts at: 0 268 708
Size: 268 440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 662 708
Size: 548 114 46
Fragments arranged by size:
548 114 46
EcoRI G'AATT_C
Cuts at: 0 404 708
Size: 404 304
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 82 708
Size: 82 626
HaeII r_GCGC'y
Cuts at: 0 467 708
Size: 467 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 605 637 708
Size: 605 32 71
Fragments arranged by size:
605 71 32
HincII GTy'rAC
Cuts at: 0 54 651 708
Size: 54 597 57
Fragments arranged by size:
597 57 54
HindIII A'AGCT_T
Cuts at: 0 559 708
Size: 559 149
HpaI GTT'AAC
Cuts at: 0 54 708
Size: 54 654
NheI G'CTAG_C
Cuts at: 0 135 708
Size: 135 573
NspI r_CATG'y
Cuts at: 0 178 708
Size: 178 530
PshAI GACnn'nnGTC
Cuts at: 0 656 708
Size: 656 52
SacI G_AGCT'C
Cuts at: 0 270 708
Size: 270 438
SapI GCTCTTCn'nnn_
Cuts at: 0 524 708
Size: 524 184
SfcI C'TryA_G
Cuts at: 0 89 479 708
Size: 89 390 229
Fragments arranged by size:
390 229 89
SmlI C'TyrA_G
Cuts at: 0 259 436 708
Size: 259 177 272
Fragments arranged by size:
272 259 177
SphI G_CATG'C
Cuts at: 0 178 708
Size: 178 530
StyI C'CwwG_G
Cuts at: 0 152 163 708
Size: 152 11 545
Fragments arranged by size:
545 152 11
TatI w'GTAC_w
Cuts at: 0 44 108 708
Size: 44 64 600
Fragments arranged by size:
600 64 44
XmnI GAAnn'nnTTC
Cuts at: 0 404 708
Size: 404 304
Enzymes that do cut:
AcuI AfeI AhdI AlfI ApoI BanII BciVI BglI
BglII BmtI Bpu10I BpuEI BsaHI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BsrDI BstYI EarI EcoICRI Eco57MI EcoRI
FalI HaeII Hin4I HincII HindIII HpaI NheI NspI
PshAI SacI SapI SfcI SmlI SphI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BclI BfrBI BlpI Bme1580I BmgBI BmrI BplI
BpmI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoNI EcoO109I EcoRV FseI FspI
FspAI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI