Restriction map for
pFN21AE4025
(Linear) (Six-base) MAPSORT of: KIEE4025.seq Check: 7265 from: 1 to: 696
KIEE4025.orf
With 182 enzymes: SgfI *
March 22, 2012 17:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 534 696
Size: 224 310 162
Fragments arranged by size:
310 224 162
AgeI A'CCGG_T
Cuts at: 0 656 696
Size: 656 40
AlwNI CAG_nnn'CTG
Cuts at: 0 259 696
Size: 259 437
ApaI G_GGCC'C
Cuts at: 0 63 696
Size: 63 633
BanI G'GyrC_C
Cuts at: 0 36 696
Size: 36 660
BanII G_rGCy'C
Cuts at: 0 7 63 696
Size: 7 56 633
Fragments arranged by size:
633 56 7
BbeI G_GCGC'C
Cuts at: 0 40 696
Size: 40 656
BbsI GAAGACnn'nnnn_
Cuts at: 0 455 696
Size: 455 241
BlpI GC'TnA_GC
Cuts at: 0 13 696
Size: 13 683
Bme1580I G_kGCm'C
Cuts at: 0 63 342 696
Size: 63 279 354
Fragments arranged by size:
354 279 63
BmgBI CAC'GTC
Cuts at: 0 98 696
Size: 98 598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 696
Size: 85 611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 415 696
Size: 142 273 281
Fragments arranged by size:
281 273 142
BsaAI yAC'GTr
Cuts at: 0 162 696
Size: 162 534
BsaBI GATnn'nnATC
Cuts at: 0 536 696
Size: 536 160
BsaHI Gr'CG_yC
Cuts at: 0 37 696
Size: 37 659
BsaWI w'CCGG_w
Cuts at: 0 143 656 696
Size: 143 513 40
Fragments arranged by size:
513 143 40
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 696
Size: 412 284
BsiHKAI G_wGCw'C
Cuts at: 0 19 696
Size: 19 677
Bsp1286I G_dGCh'C
Cuts at: 0 7 19 63 342 696
Size: 7 12 44 279 354
Fragments arranged by size:
354 279 44 12 7
BspEI T'CCGG_A
Cuts at: 0 143 696
Size: 143 553
BsrFI r'CCGG_y
Cuts at: 0 656 696
Size: 656 40
EaeI y'GGCC_r
Cuts at: 0 156 696
Size: 156 540
EarI CTCTTCn'nnn_
Cuts at: 0 639 696
Size: 639 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 224 534 696
Size: 85 139 310 162
Fragments arranged by size:
310 162 139 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 242 696
Size: 242 454
EcoO109I rG'GnC_Cy
Cuts at: 0 60 78 696
Size: 60 18 618
Fragments arranged by size:
618 60 18
HaeII r_GCGC'y
Cuts at: 0 40 696
Size: 40 656
KasI G'GCGC_C
Cuts at: 0 36 696
Size: 36 660
MscI TGG'CCA
Cuts at: 0 158 696
Size: 158 538
MspA1I CmG'CkG
Cuts at: 0 26 152 696
Size: 26 126 544
Fragments arranged by size:
544 126 26
NarI GG'CG_CC
Cuts at: 0 37 696
Size: 37 659
PpuMI rG'GwC_Cy
Cuts at: 0 78 696
Size: 78 618
PspOMI G'GGCC_C
Cuts at: 0 59 696
Size: 59 637
SanDI GG'GwC_CC
Cuts at: 0 78 696
Size: 78 618
SfoI GGC'GCC
Cuts at: 0 38 696
Size: 38 658
SmlI C'TyrA_G
Cuts at: 0 121 394 696
Size: 121 273 302
Fragments arranged by size:
302 273 121
StuI AGG'CCT
Cuts at: 0 498 696
Size: 498 198
TatI w'GTAC_w
Cuts at: 0 362 696
Size: 362 334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 138 170 696
Size: 138 32 526
Fragments arranged by size:
526 138 32
XmnI GAAnn'nnTTC
Cuts at: 0 248 696
Size: 248 448
Enzymes that do cut:
AcuI AgeI AlwNI ApaI BanI BanII BbeI BbsI
BlpI Bme1580I BmgBI BpmI BpuEI BsaAI BsaBI BsaHI
BsaWI BseRI BsiHKAI Bsp1286I BspEI BsrFI EaeI EarI
Eco57MI EcoNI EcoO109I HaeII KasI MscI MspA1I NarI
PpuMI PspOMI SanDI SfoI SmlI StuI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmrI BmtI BplI Bpu10I
BsaI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI