Restriction map for
pFN21AE4023
(Linear) (Six-base) MAPSORT of: KIEE4023.seq Check: 3155 from: 1 to: 633
KIEE4023.orf
With 182 enzymes: SgfI *
March 22, 2012 17:10 ..
AatII G_ACGT'C
Cuts at: 0 299 551 633
Size: 299 252 82
Fragments arranged by size:
299 252 82
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 607 633
Size: 79 528 26
Fragments arranged by size:
528 79 26
AfeI AGC'GCT
Cuts at: 0 582 633
Size: 582 51
AlwNI CAG_nnn'CTG
Cuts at: 0 474 633
Size: 474 159
ApaI G_GGCC'C
Cuts at: 0 18 633
Size: 18 615
AvaI C'yCGr_G
Cuts at: 0 10 133 633
Size: 10 123 500
Fragments arranged by size:
500 123 10
BamHI G'GATC_C
Cuts at: 0 368 633
Size: 368 265
BanII G_rGCy'C
Cuts at: 0 18 127 455 633
Size: 18 109 328 178
Fragments arranged by size:
328 178 109 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 543 633
Size: 239 304 90
Fragments arranged by size:
304 239 90
BglI GCCn_nnn'nGGC
Cuts at: 0 13 571 633
Size: 13 558 62
Fragments arranged by size:
558 62 13
Bme1580I G_kGCm'C
Cuts at: 0 18 633
Size: 18 615
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 520 633
Size: 520 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 633
Size: 469 164
Bpu10I CC'TnA_GC
Cuts at: 0 475 633
Size: 475 158
BsaBI GATnn'nnATC
Cuts at: 0 67 633
Size: 67 566
BsaHI Gr'CG_yC
Cuts at: 0 296 548 633
Size: 296 252 85
Fragments arranged by size:
296 252 85
BseYI C'CCAG_C
Cuts at: 0 443 633
Size: 443 190
BsiEI CG_ry'CG
Cuts at: 0 224 633
Size: 224 409
BsiHKAI G_wGCw'C
Cuts at: 0 140 284 383 633
Size: 140 144 99 250
Fragments arranged by size:
250 144 140 99
Bsp1286I G_dGCh'C
Cuts at: 0 18 127 140 284 383 455 633
Size: 18 109 13 144 99 72 178
Fragments arranged by size:
178 144 109 99 72 18 13
BspHI T'CATG_A
Cuts at: 0 299 633
Size: 299 334
BsrFI r'CCGG_y
Cuts at: 0 242 614 633
Size: 242 372 19
Fragments arranged by size:
372 242 19
BsrGI T'GTAC_A
Cuts at: 0 113 633
Size: 113 520
BssSI C'ACGA_G
Cuts at: 0 277 633
Size: 277 356
BstEII G'GTnAC_C
Cuts at: 0 198 633
Size: 198 435
BstYI r'GATC_y
Cuts at: 0 368 491 633
Size: 368 123 142
Fragments arranged by size:
368 142 123
Bsu36I CC'TnA_GG
Cuts at: 0 344 403 633
Size: 344 59 230
Fragments arranged by size:
344 230 59
BtgI C'CryG_G
Cuts at: 0 363 374 633
Size: 363 11 259
Fragments arranged by size:
363 259 11
DraIII CAC_nnn'GTG
Cuts at: 0 279 633
Size: 279 354
EaeI y'GGCC_r
Cuts at: 0 170 221 633
Size: 170 51 412
Fragments arranged by size:
412 170 51
EagI C'GGCC_G
Cuts at: 0 221 633
Size: 221 412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 510 633
Size: 510 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 469 607 633
Size: 79 390 138 26
Fragments arranged by size:
390 138 79 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 633
Size: 406 227
EcoO109I rG'GnC_Cy
Cuts at: 0 14 15 471 633
Size: 14 1 456 162
Fragments arranged by size:
456 162 14 1
HaeII r_GCGC'y
Cuts at: 0 482 584 615 633
Size: 482 102 31 18
Fragments arranged by size:
482 102 31 18
MslI CAynn'nnrTG
Cuts at: 0 463 633
Size: 463 170
MspA1I CmG'CkG
Cuts at: 0 365 423 447 633
Size: 365 58 24 186
Fragments arranged by size:
365 186 58 24
NaeI GCC'GGC
Cuts at: 0 616 633
Size: 616 17
NcoI C'CATG_G
Cuts at: 0 374 633
Size: 374 259
NgoMIV G'CCGG_C
Cuts at: 0 614 633
Size: 614 19
NspI r_CATG'y
Cuts at: 0 468 633
Size: 468 165
PfoI T'CCnGG_A
Cuts at: 0 554 633
Size: 554 79
PspOMI G'GGCC_C
Cuts at: 0 14 633
Size: 14 619
PvuII CAG'CTG
Cuts at: 0 423 447 633
Size: 423 24 186
Fragments arranged by size:
423 186 24
RsrII CG'GwC_CG
Cuts at: 0 40 633
Size: 40 593
SacII CC_GC'GG
Cuts at: 0 366 633
Size: 366 267
SmaI CCC'GGG
Cuts at: 0 12 633
Size: 12 621
StyI C'CwwG_G
Cuts at: 0 374 566 633
Size: 374 192 67
Fragments arranged by size:
374 192 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 286 633
Size: 286 347
TatI w'GTAC_w
Cuts at: 0 113 164 633
Size: 113 51 469
Fragments arranged by size:
469 113 51
Tth111I GACn'n_nGTC
Cuts at: 0 322 633
Size: 322 311
XmaI C'CCGG_G
Cuts at: 0 10 633
Size: 10 623
ZraI GAC'GTC
Cuts at: 0 297 549 633
Size: 297 252 84
Fragments arranged by size:
297 252 84
Enzymes that do cut:
AatII AcuI AfeI AlwNI ApaI AvaI BamHI BanII
BbsI BglI Bme1580I BplI BpmI Bpu10I BsaBI BsaHI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BsrFI BsrGI BssSI
BstEII BstYI Bsu36I BtgI DraIII EaeI EagI EciI
Eco57MI EcoNI EcoO109I HaeII MslI MspA1I NaeI NcoI
NgoMIV NspI PfoI PspOMI PvuII RsrII SacII SmaI
StyI TaqII TatI Tth111I XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BssHII BstAPI BstBI BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EarI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XcmI XhoI XmnI