(Linear) (Six-base) MAPSORT of: KIEE4019.seq  Check: 9419  from: 1  to: 603



KIEE4019.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     592     603 
   Size:        107     485      11

  Fragments arranged by size: 

                 485     107      11
 
ApoI r'AATT_y

Cuts at:      0     203     271     603 
   Size:        203      68     332

  Fragments arranged by size: 

                 332     203      68
 
AvaI C'yCGr_G

Cuts at:      0      54     114     603 
   Size:         54      60     489

  Fragments arranged by size: 

                 489      60      54
 
BanI G'GyrC_C

Cuts at:      0     118     603 
   Size:        118     485
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      64      98     603 
   Size:         64      34     505

  Fragments arranged by size: 

                 505      64      34
 
BclI T'GATC_A

Cuts at:      0     398     603 
   Size:        398     205
 
BglII A'GATC_T

Cuts at:      0     560     603 
   Size:        560      43
 
Bme1580I G_kGCm'C

Cuts at:      0     121     603 
   Size:        121     482
 
BmgBI CAC'GTC

Cuts at:      0     174     603 
   Size:        174     429
 
BmtI G_CTAG'C

Cuts at:      0     412     603 
   Size:        412     191
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      57     603 
   Size:         57     546
 
BsaHI Gr'CG_yC

Cuts at:      0      77     603 
   Size:         77     526
 
BsaWI w'CCGG_w

Cuts at:      0     287     590     603 
   Size:        287     303      13

  Fragments arranged by size: 

                 303     287      13
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     288     318     603 
   Size:        288      30     285

  Fragments arranged by size: 

                 288     285      30
 
BsiHKAI G_wGCw'C

Cuts at:      0      29     603 
   Size:         29     574
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     121     603 
   Size:         29      92     482

  Fragments arranged by size: 

                 482      92      29
 
BspEI T'CCGG_A

Cuts at:      0     590     603 
   Size:        590      13
 
BsrBI CCG'CTC

Cuts at:      0      37     105     603 
   Size:         37      68     498

  Fragments arranged by size: 

                 498      68      37
 
BstEII G'GTnAC_C

Cuts at:      0     108     603 
   Size:        108     495
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     552     603 
   Size:        552      51
 
BstYI r'GATC_y

Cuts at:      0     560     603 
   Size:        560      43
 
BtgI C'CryG_G

Cuts at:      0      47     603 
   Size:         47     556
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     433     603 
   Size:        433     170
 
BtsI GCAGTG_nn'

Cuts at:      0     419     603 
   Size:        419     184
 
EaeI y'GGCC_r

Cuts at:      0      32      44     603 
   Size:         32      12     559

  Fragments arranged by size: 

                 559      32      12
 
EarI CTCTTCn'nnn_

Cuts at:      0      83     568     603 
   Size:         83     485      35

  Fragments arranged by size: 

                 485      83      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     163     603 
   Size:        163     440
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     592     603 
   Size:        107     485      11

  Fragments arranged by size: 

                 485     107      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     208     240     603 
   Size:        208      32     363

  Fragments arranged by size: 

                 363     208      32
 
MscI TGG'CCA

Cuts at:      0      46     603 
   Size:         46     557
 
MslI CAynn'nnrTG

Cuts at:      0     124     172     257     603 
   Size:        124      48      85     346

  Fragments arranged by size: 

                 346     124      85      48
 
MspA1I CmG'CkG

Cuts at:      0     427     603 
   Size:        427     176
 
NheI G'CTAG_C

Cuts at:      0     408     603 
   Size:        408     195
 
PfoI T'CCnGG_A

Cuts at:      0     161     458     603 
   Size:        161     297     145

  Fragments arranged by size: 

                 297     161     145
 
PvuII CAG'CTG

Cuts at:      0     427     603 
   Size:        427     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     288     320     603 
   Size:        288      32     283

  Fragments arranged by size: 

                 288     283      32
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BanI     BcgI     BclI    BglII Bme1580I
    BmgBI     BmtI     BsaI    BsaHI    BsaWI    BsaXI  BsiHKAI Bsp1286I
    BspEI    BsrBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     EaeI     EarI     EciI  Eco57MI    Hin4I     MscI     MslI   MspA1I
     NheI     PfoI    PvuII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI    BciVI    BfrBI     BglI     BlpI     BmrI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI