Restriction map for
pFN21AE4017
(Linear) (Six-base) MAPSORT of: KIEE4017.seq Check: 1994 from: 1 to: 591
KIEE4017.orf
With 182 enzymes: SgfI *
March 22, 2012 17:09 ..
AflIII A'CryG_T
Cuts at: 0 437 591
Size: 437 154
ApaI G_GGCC'C
Cuts at: 0 265 591
Size: 265 326
AseI AT'TA_AT
Cuts at: 0 463 591
Size: 463 128
AvaI C'yCGr_G
Cuts at: 0 143 591
Size: 143 448
BanI G'GyrC_C
Cuts at: 0 34 48 196 591
Size: 34 14 148 395
Fragments arranged by size:
395 148 34 14
BanII G_rGCy'C
Cuts at: 0 142 265 591
Size: 142 123 326
Fragments arranged by size:
326 142 123
BbeI G_GCGC'C
Cuts at: 0 38 591
Size: 38 553
BbsI GAAGACnn'nnnn_
Cuts at: 0 112 591
Size: 112 479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 377 411 591
Size: 377 34 180
Fragments arranged by size:
377 180 34
BlpI GC'TnA_GC
Cuts at: 0 543 591
Size: 543 48
Bme1580I G_kGCm'C
Cuts at: 0 199 265 591
Size: 199 66 326
Fragments arranged by size:
326 199 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 156 591
Size: 156 435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 591
Size: 550 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 591
Size: 45 546
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 591
Size: 370 221
BsaBI GATnn'nnATC
Cuts at: 0 235 591
Size: 235 356
BsaHI Gr'CG_yC
Cuts at: 0 35 98 317 591
Size: 35 63 219 274
Fragments arranged by size:
274 219 63 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 442 472 591
Size: 442 30 119
Fragments arranged by size:
442 119 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 463 591
Size: 463 128
BseYI C'CCAG_C
Cuts at: 0 84 332 591
Size: 84 248 259
Fragments arranged by size:
259 248 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 591
Size: 373 218
BsiHKAI G_wGCw'C
Cuts at: 0 142 591
Size: 142 449
Bsp1286I G_dGCh'C
Cuts at: 0 142 199 265 591
Size: 142 57 66 326
Fragments arranged by size:
326 142 66 57
BsrDI GCAATG_nn'
Cuts at: 0 482 591
Size: 482 109
BsrFI r'CCGG_y
Cuts at: 0 10 591
Size: 10 581
BtgI C'CryG_G
Cuts at: 0 30 92 188 443 509 591
Size: 30 62 96 255 66 82
Fragments arranged by size:
255 96 82 66 62 30
EaeI y'GGCC_r
Cuts at: 0 8 591
Size: 8 583
EarI CTCTTCn'nnn_
Cuts at: 0 415 591
Size: 415 176
EcoICRI GAG'CTC
Cuts at: 0 140 591
Size: 140 451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 591
Size: 550 41
EcoO109I rG'GnC_Cy
Cuts at: 0 262 591
Size: 262 329
HaeII r_GCGC'y
Cuts at: 0 38 527 591
Size: 38 489 64
Fragments arranged by size:
489 64 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 591
Size: 442 32 117
Fragments arranged by size:
442 117 32
KasI G'GCGC_C
Cuts at: 0 34 591
Size: 34 557
MspA1I CmG'CkG
Cuts at: 0 32 591
Size: 32 559
NarI GG'CG_CC
Cuts at: 0 35 591
Size: 35 556
NspI r_CATG'y
Cuts at: 0 441 591
Size: 441 150
PciI A'CATG_T
Cuts at: 0 437 591
Size: 437 154
PfoI T'CCnGG_A
Cuts at: 0 239 591
Size: 239 352
PspOMI G'GGCC_C
Cuts at: 0 261 591
Size: 261 330
PstI C_TGCA'G
Cuts at: 0 131 378 591
Size: 131 247 213
Fragments arranged by size:
247 213 131
SacI G_AGCT'C
Cuts at: 0 142 591
Size: 142 449
SacII CC_GC'GG
Cuts at: 0 33 591
Size: 33 558
SfcI C'TryA_G
Cuts at: 0 127 374 591
Size: 127 247 217
Fragments arranged by size:
247 217 127
SfoI GGC'GCC
Cuts at: 0 36 591
Size: 36 555
SmaI CCC'GGG
Cuts at: 0 145 591
Size: 145 446
SmlI C'TyrA_G
Cuts at: 0 24 591
Size: 24 567
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 346 589 591
Size: 346 243 2
Fragments arranged by size:
346 243 2
XmaI C'CCGG_G
Cuts at: 0 143 591
Size: 143 448
Enzymes that do cut:
AflIII ApaI AseI AvaI BanI BanII BbeI BbsI
BcgI BlpI Bme1580I BplI BpmI BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI
BsrFI BtgI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI MspA1I NarI NspI PciI PfoI PspOMI
PstI SacI SacII SfcI SfoI SmaI SmlI TaqII
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I BsaAI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI