(Linear) (Six-base) MAPSORT of: KIEE4016.seq  Check: 1640  from: 1  to: 585



KIEE4016.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:09  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     358     585 
   Size:        358     227
 
ApoI r'AATT_y

Cuts at:      0     523     585 
   Size:        523      62
 
AvaI C'yCGr_G

Cuts at:      0       8      40     585 
   Size:          8      32     545

  Fragments arranged by size: 

                 545      32       8
 
BanII G_rGCy'C

Cuts at:      0      41     585 
   Size:         41     544
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     408     442     585 
   Size:        408      34     143

  Fragments arranged by size: 

                 408     143      34
 
BclI T'GATC_A

Cuts at:      0     489     585 
   Size:        489      96
 
BglII A'GATC_T

Cuts at:      0     386     585 
   Size:        386     199
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     193     585 
   Size:        193     392
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      52     585 
   Size:         52     533
 
BsaWI w'CCGG_w

Cuts at:      0     191     585 
   Size:        191     394
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     585 
   Size:         58     527
 
BseYI C'CCAG_C

Cuts at:      0     453     585 
   Size:        453     132
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     585 
   Size:        328     257
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     585 
   Size:         41     544
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     585 
   Size:         41     544
 
BsrFI r'CCGG_y

Cuts at:      0      61      98     585 
   Size:         61      37     487

  Fragments arranged by size: 

                 487      61      37
 
BstYI r'GATC_y

Cuts at:      0     386     585 
   Size:        386     199
 
BtgI C'CryG_G

Cuts at:      0     240     585 
   Size:        240     345
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     447     585 
   Size:        447     138
 
EarI CTCTTCn'nnn_

Cuts at:      0      29     585 
   Size:         29     556
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     259     585 
   Size:        259     326
 
EcoICRI GAG'CTC

Cuts at:      0      39     585 
   Size:         39     546
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     422     454     585 
   Size:        422      32     131

  Fragments arranged by size: 

                 422     131      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     408     585 
   Size:        408     177
 
MspA1I CmG'CkG

Cuts at:      0     453     585 
   Size:        453     132
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     246     278     585 
   Size:        246      32     307

  Fragments arranged by size: 

                 307     246      32
 
PspXI vC'TCGA_Gb

Cuts at:      0      40     585 
   Size:         40     545
 
PvuII CAG'CTG

Cuts at:      0     453     585 
   Size:        453     132
 
SacI G_AGCT'C

Cuts at:      0      41     585 
   Size:         41     544
 
SapI GCTCTTCn'nnn_

Cuts at:      0      29     585 
   Size:         29     556
 
SexAI A'CCwGG_T

Cuts at:      0     227     585 
   Size:        227     358
 
SfcI C'TryA_G

Cuts at:      0      93     585 
   Size:         93     492
 
SmlI C'TyrA_G

Cuts at:      0      40     585 
   Size:         40     545
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     180     585 
   Size:        180     405
 
XhoI C'TCGA_G

Cuts at:      0      40     585 
   Size:         40     545
 

 Enzymes that do cut:

     AlfI     ApoI     AvaI    BanII     BcgI     BclI    BglII     BmrI
     BsaI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrFI
    BstYI     BtgI    BtgZI     EarI     EciI  EcoICRI    Hin4I     MmeI
   MspA1I     PpiI    PspXI    PvuII     SacI     SapI    SexAI     SfcI
     SmlI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XmaI     XmnI
     ZraI