(Linear) (Six-base) MAPSORT of: KIEE4013.seq  Check: 9603  from: 1  to: 564



KIEE4013.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:08  ..

AccI GT'mk_AC

Cuts at:      0     418     564 
   Size:        418     146
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     396     564 
   Size:        396     168
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     395     427     564 
   Size:        395      32     137

  Fragments arranged by size: 

                 395     137      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      78     564 
   Size:         78     486
 
ApaI G_GGCC'C

Cuts at:      0     453     564 
   Size:        453     111
 
ApoI r'AATT_y

Cuts at:      0     189     268     564 
   Size:        189      79     296

  Fragments arranged by size: 

                 296     189      79
 
BanII G_rGCy'C

Cuts at:      0     453     564 
   Size:        453     111
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     182     564 
   Size:        182     382
 
BglII A'GATC_T

Cuts at:      0     116     564 
   Size:        116     448
 
Bme1580I G_kGCm'C

Cuts at:      0     453     564 
   Size:        453     111
 
BmgBI CAC'GTC

Cuts at:      0     444     564 
   Size:        444     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     484     490     492     564 
   Size:        173     311       6       2      72

  Fragments arranged by size: 

                 311     173      72       6       2
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      95     564 
   Size:         95     469
 
BsaHI Gr'CG_yC

Cuts at:      0       4     564 
   Size:          4     560
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     420     450     564 
   Size:        420      30     114

  Fragments arranged by size: 

                 420     114      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     453     564 
   Size:        453     111
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     430     564 
   Size:        430     134
 
BstEII G'GTnAC_C

Cuts at:      0      81     564 
   Size:         81     483
 
BstYI r'GATC_y

Cuts at:      0     116     327     564 
   Size:        116     211     237

  Fragments arranged by size: 

                 237     211     116
 
ClaI AT'CG_AT

Cuts at:      0     138     564 
   Size:        138     426
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     472     564 
   Size:        472      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     484     490     492     564 
   Size:        173     311       6       2      72

  Fragments arranged by size: 

                 311     173      72       6       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     457     564 
   Size:        457     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0     449     450     564 
   Size:        449       1     114

  Fragments arranged by size: 

                 449     114       1
 
EcoRI G'AATT_C

Cuts at:      0     189     564 
   Size:        189     375
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     120     124     152     156     564 
   Size:        120       4      28       4     408

  Fragments arranged by size: 

                 408     120      28       4       4
 
HincII GTy'rAC

Cuts at:      0     419     564 
   Size:        419     145
 
MspA1I CmG'CkG

Cuts at:      0     480     510     564 
   Size:        480      30      54

  Fragments arranged by size: 

                 480      54      30
 
NspI r_CATG'y

Cuts at:      0     387     564 
   Size:        387     177
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      78     564 
   Size:         78     486
 
PspOMI G'GGCC_C

Cuts at:      0     449     564 
   Size:        449     115
 
PvuII CAG'CTG

Cuts at:      0     480     564 
   Size:        480      84
 
SalI G'TCGA_C

Cuts at:      0     417     564 
   Size:        417     147
 
SfcI C'TryA_G

Cuts at:      0      35     564 
   Size:         35     529
 
SspI AAT'ATT

Cuts at:      0     221     236     564 
   Size:        221      15     328

  Fragments arranged by size: 

                 328     221      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     420     452     564 
   Size:        420      32     112

  Fragments arranged by size: 

                 420     112      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     244     564 
   Size:        244     320
 

 Enzymes that do cut:

     AccI     AlfI     AloI    AlwNI     ApaI     ApoI    BanII     BbsI
    BglII Bme1580I    BmgBI     BpmI     BsaI    BsaHI    BsaXI Bsp1286I
    BspMI   BstEII    BstYI     ClaI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI    Hin4I   HincII   MspA1I     NspI    PflMI   PspOMI    PvuII
     SalI     SfcI     SspI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI