Restriction map for
pFN21AE4009
(Linear) (Six-base) MAPSORT of: KIEE4009.seq Check: 3840 from: 1 to: 2484
KIEE4009.orf
With 182 enzymes: SgfI *
March 22, 2012 17:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 678 917 1074 2484
Size: 678 239 157 1410
Fragments arranged by size:
1410 678 239 157
AccI GT'mk_AC
Cuts at: 0 938 2360 2484
Size: 938 1422 124
Fragments arranged by size:
1422 938 124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 819 990 2484
Size: 95 724 171 1494
Fragments arranged by size:
1494 724 171 95
AfeI AGC'GCT
Cuts at: 0 1924 2484
Size: 1924 560
AflIII A'CryG_T
Cuts at: 0 452 626 2484
Size: 452 174 1858
Fragments arranged by size:
1858 452 174
AleI CACnn'nnGTG
Cuts at: 0 964 2484
Size: 964 1520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1430 2484
Size: 1430 1054
AlwNI CAG_nnn'CTG
Cuts at: 0 73 556 780 869 1326 1341 2484
Size: 73 483 224 89 457 15 1143
Fragments arranged by size:
1143 483 457 224 89 73 15
ApaI G_GGCC'C
Cuts at: 0 1023 1993 2060 2484
Size: 1023 970 67 424
Fragments arranged by size:
1023 970 424 67
ApaLI G'TGCA_C
Cuts at: 0 1735 2484
Size: 1735 749
ApoI r'AATT_y
Cuts at: 0 13 102 715 2484
Size: 13 89 613 1769
Fragments arranged by size:
1769 613 89 13
AvaI C'yCGr_G
Cuts at: 0 235 435 826 854 1258 1438 1747 2029
Size: 235 200 391 28 404 180 309 282
Cuts at: 2029 2071 2484
Size: 42 413
Fragments arranged by size:
413 404 391 309 282 235 200 180
42 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2385 2418 2484
Size: 2385 33 66
Fragments arranged by size:
2385 66 33
BanI G'GyrC_C
Cuts at: 0 1593 1641 1648 1931 2142 2423 2484
Size: 1593 48 7 283 211 281 61
Fragments arranged by size:
1593 283 281 211 61 48 7
BanII G_rGCy'C
Cuts at: 0 353 464 697 758 833 1023 1265 1439
Size: 353 111 233 61 75 190 242 174
Cuts at: 1439 1754 1993 2060 2484
Size: 315 239 67 424
Fragments arranged by size:
424 353 315 242 239 233 190 174
111 75 67 61
BbeI G_GCGC'C
Cuts at: 0 1935 2484
Size: 1935 549
BbsI GAAGACnn'nnnn_
Cuts at: 0 1227 2484
Size: 1227 1257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1137 1171 2484
Size: 1137 34 1313
Fragments arranged by size:
1313 1137 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2413 2484
Size: 2413 71
BglII A'GATC_T
Cuts at: 0 1367 1583 2484
Size: 1367 216 901
Fragments arranged by size:
1367 901 216
BlpI GC'TnA_GC
Cuts at: 0 1663 2484
Size: 1663 821
Bme1580I G_kGCm'C
Cuts at: 0 1023 1419 1598 1646 1739 1993 2060 2484
Size: 1023 396 179 48 93 254 67 424
Fragments arranged by size:
1023 424 396 254 179 93 67 48
BmgBI CAC'GTC
Cuts at: 0 620 843 2484
Size: 620 223 1641
Fragments arranged by size:
1641 620 223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 291 2484
Size: 141 150 2193
Fragments arranged by size:
2193 150 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 359 916 1488 1918 2484
Size: 144 215 557 572 430 566
Fragments arranged by size:
572 566 557 430 215 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 742 2443 2484
Size: 685 57 1701 41
Fragments arranged by size:
1701 685 57 41
BsaAI yAC'GTr
Cuts at: 0 1170 1851 1983 2349 2484
Size: 1170 681 132 366 135
Fragments arranged by size:
1170 681 366 135 132
BsaHI Gr'CG_yC
Cuts at: 0 1932 2484
Size: 1932 552
BsaWI w'CCGG_w
Cuts at: 0 1142 2484
Size: 1142 1342
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 491 521 643 673 1117 1147 1195 1225
Size: 491 30 122 30 444 30 48 30
Cuts at: 1225 1486 1516 2371 2401 2484
Size: 261 30 855 30 83
Fragments arranged by size:
855 491 444 261 122 83 48 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 234 358 361 1138 1203 1231 1405 1408
Size: 234 124 3 777 65 28 174 3
Cuts at: 1408 1491 2059 2091 2218 2395 2484
Size: 83 568 32 127 177 89
Fragments arranged by size:
777 568 234 177 174 127 124 89
83 65 32 28 3 3
BseYI C'CCAG_C
Cuts at: 0 632 989 1022 1048 1281 1418 1625 2407
Size: 632 357 33 26 233 137 207 782
Cuts at: 2407 2484
Size: 77
Fragments arranged by size:
782 632 357 233 207 137 77 33
26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 835 889 1873 2019 2209 2484
Size: 622 213 54 984 146 190 275
Fragments arranged by size:
984 622 275 213 190 146 54
BsiEI CG_ry'CG
Cuts at: 0 1564 1884 2484
Size: 1564 320 600
Fragments arranged by size:
1564 600 320
BsiHKAI G_wGCw'C
Cuts at: 0 464 758 833 1265 1439 1739 1754 2484
Size: 464 294 75 432 174 300 15 730
Fragments arranged by size:
730 464 432 300 294 174 75 15
Bsp1286I G_dGCh'C
Cuts at: 0 353 464 697 758 833 1023 1265 1419
Size: 353 111 233 61 75 190 242 154
Cuts at: 1419 1439 1598 1646 1739 1754 1993 2060 2484
Size: 20 159 48 93 15 239 67 424
Fragments arranged by size:
424 353 242 239 233 190 159 154
111 93 75 67 61 48 20 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 452 662 678 917 1074 1778 2165 2484
Size: 452 210 16 239 157 704 387 319
Fragments arranged by size:
704 452 387 319 239 210 157 16
BsrBI CCG'CTC
Cuts at: 0 1560 1574 2484
Size: 1560 14 910
Fragments arranged by size:
1560 910 14
BsrDI GCAATG_nn'
Cuts at: 0 297 333 2484
Size: 297 36 2151
Fragments arranged by size:
2151 297 36
BsrFI r'CCGG_y
Cuts at: 0 304 1247 1928 2484
Size: 304 943 681 556
Fragments arranged by size:
943 681 556 304
BssSI C'ACGA_G
Cuts at: 0 1762 2484
Size: 1762 722
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1341 2484
Size: 1341 1143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 292 705 2484
Size: 292 413 1779
Fragments arranged by size:
1779 413 292
BstYI r'GATC_y
Cuts at: 0 1367 1583 1619 2099 2484
Size: 1367 216 36 480 385
Fragments arranged by size:
1367 480 385 216 36
BtgI C'CryG_G
Cuts at: 0 2204 2484
Size: 2204 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 200 1140 2484
Size: 200 940 1344
Fragments arranged by size:
1344 940 200
BtsI GCAGTG_nn'
Cuts at: 0 143 499 870 1417 1470 2062 2484
Size: 143 356 371 547 53 592 422
Fragments arranged by size:
592 547 422 371 356 143 53
ClaI AT'CG_AT
Cuts at: 0 2414 2484
Size: 2414 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2456 2484
Size: 2456 28
DraIII CAC_nnn'GTG
Cuts at: 0 129 1173 1851 2349 2484
Size: 129 1044 678 498 135
Fragments arranged by size:
1044 678 498 135 129
EaeI y'GGCC_r
Cuts at: 0 710 881 1053 1095 1561 2484
Size: 710 171 172 42 466 923
Fragments arranged by size:
923 710 466 172 171 42
EagI C'GGCC_G
Cuts at: 0 1561 2484
Size: 1561 923
EarI CTCTTCn'nnn_
Cuts at: 0 211 826 838 1258 1270 1759 2257 2484
Size: 211 615 12 420 12 489 498 227
Fragments arranged by size:
615 498 489 420 227 211 12 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1831 2224 2329 2484
Size: 1831 393 105 155
Fragments arranged by size:
1831 393 155 105
EcoICRI GAG'CTC
Cuts at: 0 462 756 831 1263 1437 1752 2484
Size: 462 294 75 432 174 315 732
Fragments arranged by size:
732 462 432 315 294 174 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 144 359 819 916 990 1488 1918
Size: 95 49 215 460 97 74 498 430
Cuts at: 1918 2484
Size: 566
Fragments arranged by size:
566 498 460 430 215 97 95 74
49
EcoO109I rG'GnC_Cy
Cuts at: 0 441 792 1128 1185 1443 1990 2056 2057
Size: 441 351 336 57 258 547 66 1
Cuts at: 2057 2484
Size: 427
Fragments arranged by size:
547 441 427 351 336 258 66 57
1
EcoRI G'AATT_C
Cuts at: 0 13 102 2484
Size: 13 89 2382
Fragments arranged by size:
2382 89 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2121 2484
Size: 2121 363
FspI TGC'GCA
Cuts at: 0 1774 2484
Size: 1774 710
HaeII r_GCGC'y
Cuts at: 0 1926 1935 1956 2484
Size: 1926 9 21 528
Fragments arranged by size:
1926 528 21 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 1117 1149 1195 1227 1507 1539
Size: 643 32 442 32 46 32 280 32
Cuts at: 1539 2484
Size: 945
Fragments arranged by size:
945 643 442 280 46 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1931 2484
Size: 1931 553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 2484
Size: 647 1837
MscI TGG'CCA
Cuts at: 0 712 2484
Size: 712 1772
MslI CAynn'nnrTG
Cuts at: 0 703 964 1525 2484
Size: 703 261 561 959
Fragments arranged by size:
959 703 561 261
MspA1I CmG'CkG
Cuts at: 0 177 636 993 1026 1052 1298 1338 1793
Size: 177 459 357 33 26 246 40 455
Cuts at: 1793 1815 1857 2484
Size: 22 42 627
Fragments arranged by size:
627 459 455 357 246 177 42 40
33 26 22
NaeI GCC'GGC
Cuts at: 0 306 1930 2484
Size: 306 1624 554
Fragments arranged by size:
1624 554 306
NarI GG'CG_CC
Cuts at: 0 1932 2484
Size: 1932 552
NgoMIV G'CCGG_C
Cuts at: 0 304 1928 2484
Size: 304 1624 556
Fragments arranged by size:
1624 556 304
NspI r_CATG'y
Cuts at: 0 258 606 630 1386 2484
Size: 258 348 24 756 1098
Fragments arranged by size:
1098 756 348 258 24
PasI CC'CwG_GG
Cuts at: 0 1800 2484
Size: 1800 684
PciI A'CATG_T
Cuts at: 0 626 2484
Size: 626 1858
PflMI CCAn_nnn'nTGG
Cuts at: 0 1104 1173 2349 2484
Size: 1104 69 1176 135
Fragments arranged by size:
1176 1104 135 69
PfoI T'CCnGG_A
Cuts at: 0 106 1103 1199 2039 2484
Size: 106 997 96 840 445
Fragments arranged by size:
997 840 445 106 96
PmlI CAC'GTG
Cuts at: 0 1170 1983 2484
Size: 1170 813 501
Fragments arranged by size:
1170 813 501
PpuMI rG'GwC_Cy
Cuts at: 0 441 792 1185 2484
Size: 441 351 393 1299
Fragments arranged by size:
1299 441 393 351
PspOMI G'GGCC_C
Cuts at: 0 1019 1989 2056 2484
Size: 1019 970 67 428
Fragments arranged by size:
1019 970 428 67
PspXI vC'TCGA_Gb
Cuts at: 0 1438 2484
Size: 1438 1046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 20 52 2484
Size: 20 32 2432
Fragments arranged by size:
2432 32 20
PstI C_TGCA'G
Cuts at: 0 155 865 914 1346 1409 1792 2279 2484
Size: 155 710 49 432 63 383 487 205
Fragments arranged by size:
710 487 432 383 205 155 63 49
PvuII CAG'CTG
Cuts at: 0 177 636 993 1298 1815 1857 2484
Size: 177 459 357 305 517 42 627
Fragments arranged by size:
627 517 459 357 305 177 42
SacI G_AGCT'C
Cuts at: 0 464 758 833 1265 1439 1754 2484
Size: 464 294 75 432 174 315 730
Fragments arranged by size:
730 464 432 315 294 174 75
SapI GCTCTTCn'nnn_
Cuts at: 0 826 838 1258 1270 1759 2484
Size: 826 12 420 12 489 725
Fragments arranged by size:
826 725 489 420 12 12
SbfI CC_TGCA'GG
Cuts at: 0 155 2484
Size: 155 2329
SfcI C'TryA_G
Cuts at: 0 151 861 910 1342 1405 1788 2275 2484
Size: 151 710 49 432 63 383 487 209
Fragments arranged by size:
710 487 432 383 209 151 63 49
SfoI GGC'GCC
Cuts at: 0 1933 2484
Size: 1933 551
SmaI CCC'GGG
Cuts at: 0 856 2484
Size: 856 1628
SmlI C'TyrA_G
Cuts at: 0 664 757 1438 2458 2484
Size: 664 93 681 1020 26
Fragments arranged by size:
1020 681 664 93 26
SphI G_CATG'C
Cuts at: 0 258 2484
Size: 258 2226
StuI AGG'CCT
Cuts at: 0 1399 2484
Size: 1399 1085
StyI C'CwwG_G
Cuts at: 0 285 2105 2484
Size: 285 1820 379
Fragments arranged by size:
1820 379 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1273 2484
Size: 1273 1211
TatI w'GTAC_w
Cuts at: 0 1292 1781 2279 2484
Size: 1292 489 498 205
Fragments arranged by size:
1292 498 489 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 2371 2403 2484
Size: 491 32 1848 32 81
Fragments arranged by size:
1848 491 81 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 136 202 1885 2484
Size: 136 66 1683 599
Fragments arranged by size:
1683 599 136 66
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 705 891 2484
Size: 705 186 1593
Fragments arranged by size:
1593 705 186
XhoI C'TCGA_G
Cuts at: 0 1438 2484
Size: 1438 1046
XmaI C'CCGG_G
Cuts at: 0 854 2484
Size: 854 1630
XmnI GAAnn'nnTTC
Cuts at: 0 35 595 1000 2484
Size: 35 560 405 1484
Fragments arranged by size:
1484 560 405 35
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BaeI BanI BanII BbeI
BbsI BcgI BciVI BglII BlpI Bme1580I BmgBI BplI
BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PsrI
PstI PvuII SacI SapI SbfI SfcI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI AscI
AseI AsiSI AvrII BamHI BbvCI BclI BfrBI BglI
BmrI BmtI Bpu10I BsaI BsaBI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
DraI DrdI EcoNI EcoRV FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PvuI
RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XbaI ZraI