(Linear) (Six-base) MAPSORT of: KIEE4006.seq  Check: 8612  from: 1  to: 378



KIEE4006.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:07  ..

AflIII A'CryG_T

Cuts at:      0     159     378 
   Size:        159     219
 
AlwNI CAG_nnn'CTG

Cuts at:      0     142     378 
   Size:        142     236
 
ApaI G_GGCC'C

Cuts at:      0      13     378 
   Size:         13     365
 
AvaI C'yCGr_G

Cuts at:      0      13      22     110     378 
   Size:         13       9      88     268

  Fragments arranged by size: 

                 268      88      13       9
 
BanII G_rGCy'C

Cuts at:      0      13     269     378 
   Size:         13     256     109

  Fragments arranged by size: 

                 256     109      13
 
BbvCI CC'TCA_GC

Cuts at:      0     361     378 
   Size:        361      17
 
Bme1580I G_kGCm'C

Cuts at:      0      13     378 
   Size:         13     365
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     355     378 
   Size:         60     295      23

  Fragments arranged by size: 

                 295      60      23
 
Bpu10I CC'TnA_GC

Cuts at:      0     361     378 
   Size:        361      17
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     330     378 
   Size:        330      48
 
BsaAI yAC'GTr

Cuts at:      0     162     378 
   Size:        162     216
 
BsaHI Gr'CG_yC

Cuts at:      0     107     378 
   Size:        107     271
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238     241     244     274     378 
   Size:        238       3       3      30     104

  Fragments arranged by size: 

                 238     104      30       3       3
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     269     378 
   Size:         13     256     109

  Fragments arranged by size: 

                 256     109      13
 
BspHI T'CATG_A

Cuts at:      0      86     378 
   Size:         86     292
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     125     378 
   Size:        125     253
 
BsrBI CCG'CTC

Cuts at:      0     300     378 
   Size:        300      78
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     240     378 
   Size:        240     138
 
Bsu36I CC'TnA_GG

Cuts at:      0     272     378 
   Size:        272     106
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     331     378 
   Size:        331      47
 
BtsI GCAGTG_nn'

Cuts at:      0     152     310     378 
   Size:        152     158      68

  Fragments arranged by size: 

                 158     152      68
 
EaeI y'GGCC_r

Cuts at:      0     123     378 
   Size:        123     255
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     355     378 
   Size:         60     295      23

  Fragments arranged by size: 

                 295      60      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     244     378 
   Size:        244     134
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10     114     378 
   Size:         10     104     264

  Fragments arranged by size: 

                 264     104      10
 
HaeII r_GCGC'y

Cuts at:      0      52     378 
   Size:         52     326
 
MscI TGG'CCA

Cuts at:      0     125     378 
   Size:        125     253
 
MspA1I CmG'CkG

Cuts at:      0     142     378 
   Size:        142     236
 
NspI r_CATG'y

Cuts at:      0     369     378 
   Size:        369       9
 
PmlI CAC'GTG

Cuts at:      0     162     378 
   Size:        162     216
 
PpuMI rG'GwC_Cy

Cuts at:      0     114     378 
   Size:        114     264
 
PspOMI G'GGCC_C

Cuts at:      0       9     378 
   Size:          9     369
 
PstI C_TGCA'G

Cuts at:      0     147     245     320     377     378 
   Size:        147      98      75      57       1

  Fragments arranged by size: 

                 147      98      75      57       1
 
PvuII CAG'CTG

Cuts at:      0     142     378 
   Size:        142     236
 
SbfI CC_TGCA'GG

Cuts at:      0     245     378 
   Size:        245     133
 
SfcI C'TryA_G

Cuts at:      0     143     241     316     373     378 
   Size:        143      98      75      57       5

  Fragments arranged by size: 

                 143      98      75      57       5
 
SmaI CCC'GGG

Cuts at:      0     112     378 
   Size:        112     266
 
SphI G_CATG'C

Cuts at:      0     369     378 
   Size:        369       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     205     378 
   Size:        205     173
 
XmaI C'CCGG_G

Cuts at:      0     110     378 
   Size:        110     268
 

 Enzymes that do cut:

   AflIII    AlwNI     ApaI     AvaI    BanII    BbvCI Bme1580I     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BseRI Bsp1286I    BspHI    BspMI
    BsrBI   BstAPI   Bsu36I    BtgZI     BtsI     EaeI  Eco57MI    EcoNI
 EcoO109I    HaeII     MscI   MspA1I     NspI     PmlI    PpuMI   PspOMI
     PstI    PvuII     SbfI     SfcI     SmaI     SphI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaBI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI