Restriction map for
pFN21AE3994
(Linear) (Six-base) MAPSORT of: KIEE3994.seq Check: 8124 from: 1 to: 1263
KIEE3994.orf
With 182 enzymes: SgfI *
March 22, 2012 17:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 652 1263
Size: 652 611
AatII G_ACGT'C
Cuts at: 0 608 1263
Size: 608 655
Acc65I G'GTAC_C
Cuts at: 0 1220 1263
Size: 1220 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1144 1234 1263
Size: 820 324 90 29
Fragments arranged by size:
820 324 90 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 232 1263
Size: 232 1031
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 622 654 1263
Size: 622 32 609
Fragments arranged by size:
622 609 32
AlwNI CAG_nnn'CTG
Cuts at: 0 48 1263
Size: 48 1215
AvaI C'yCGr_G
Cuts at: 0 88 1263
Size: 88 1175
BamHI G'GATC_C
Cuts at: 0 77 401 1263
Size: 77 324 862
Fragments arranged by size:
862 324 77
BanI G'GyrC_C
Cuts at: 0 1220 1263
Size: 1220 43
BanII G_rGCy'C
Cuts at: 0 70 1263
Size: 70 1193
BbsI GAAGACnn'nnnn_
Cuts at: 0 299 950 1263
Size: 299 651 313
Fragments arranged by size:
651 313 299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 937 971 1263
Size: 937 34 292
Fragments arranged by size:
937 292 34
BclI T'GATC_A
Cuts at: 0 701 1263
Size: 701 562
BglI GCCn_nnn'nGGC
Cuts at: 0 1209 1263
Size: 1209 54
BlpI GC'TnA_GC
Cuts at: 0 230 1263
Size: 230 1033
Bme1580I G_kGCm'C
Cuts at: 0 769 1263
Size: 769 494
BmrI ACTGGGnnnn_n'
Cuts at: 0 110 292 1263
Size: 110 182 971
Fragments arranged by size:
971 182 110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 862 1003 1123 1263
Size: 187 675 141 120 140
Fragments arranged by size:
675 187 141 140 120
Bpu10I CC'TnA_GC
Cuts at: 0 443 1228 1263
Size: 443 785 35
Fragments arranged by size:
785 443 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1108 1113 1263
Size: 205 903 5 150
Fragments arranged by size:
903 205 150 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 1263
Size: 661 602
BsaHI Gr'CG_yC
Cuts at: 0 203 605 1263
Size: 203 402 658
Fragments arranged by size:
658 402 203
BsaWI w'CCGG_w
Cuts at: 0 20 398 847 1263
Size: 20 378 449 416
Fragments arranged by size:
449 416 378 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 473 503 850 880 1228 1258 1263
Size: 473 30 347 30 348 30 5
Fragments arranged by size:
473 348 347 30 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 451 703 1263
Size: 148 303 252 560
Fragments arranged by size:
560 303 252 148
BseYI C'CCAG_C
Cuts at: 0 234 757 768 1263
Size: 234 523 11 495
Fragments arranged by size:
523 495 234 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 799 856 921 1263
Size: 574 225 57 65 342
Fragments arranged by size:
574 342 225 65 57
BsiEI CG_ry'CG
Cuts at: 0 254 1263
Size: 254 1009
BsiHKAI G_wGCw'C
Cuts at: 0 1238 1263
Size: 1238 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 586 1263
Size: 586 677
Bsp1286I G_dGCh'C
Cuts at: 0 70 769 1238 1263
Size: 70 699 469 25
Fragments arranged by size:
699 469 70 25
BspEI T'CCGG_A
Cuts at: 0 20 847 1263
Size: 20 827 416
Fragments arranged by size:
827 416 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 652 1263
Size: 652 611
BsrBI CCG'CTC
Cuts at: 0 93 1263
Size: 93 1170
BsrFI r'CCGG_y
Cuts at: 0 110 1263
Size: 110 1153
BssSI C'ACGA_G
Cuts at: 0 406 775 1263
Size: 406 369 488
Fragments arranged by size:
488 406 369
BstEII G'GTnAC_C
Cuts at: 0 999 1263
Size: 999 264
BstYI r'GATC_y
Cuts at: 0 77 401 557 990 1070 1263
Size: 77 324 156 433 80 193
Fragments arranged by size:
433 324 193 156 80 77
BtgI C'CryG_G
Cuts at: 0 671 1165 1247 1263
Size: 671 494 82 16
Fragments arranged by size:
671 494 82 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 309 1263
Size: 309 954
DraI TTT'AAA
Cuts at: 0 810 1263
Size: 810 453
DraIII CAC_nnn'GTG
Cuts at: 0 1050 1263
Size: 1050 213
EaeI y'GGCC_r
Cuts at: 0 251 428 536 1142 1263
Size: 251 177 108 606 121
Fragments arranged by size:
606 251 177 121 108
EagI C'GGCC_G
Cuts at: 0 251 1263
Size: 251 1012
EarI CTCTTCn'nnn_
Cuts at: 0 796 1263
Size: 796 467
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1078 1263
Size: 1078 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 820 862 1003 1123 1144 1234 1263
Size: 187 633 42 141 120 21 90 29
Fragments arranged by size:
633 187 141 120 90 42 29 21
EcoO109I rG'GnC_Cy
Cuts at: 0 84 1077 1263
Size: 84 993 186
Fragments arranged by size:
993 186 84
EcoRV GAT'ATC
Cuts at: 0 1041 1263
Size: 1041 222
FspI TGC'GCA
Cuts at: 0 970 1263
Size: 970 293
HaeII r_GCGC'y
Cuts at: 0 450 468 1263
Size: 450 18 795
Fragments arranged by size:
795 450 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 473 505 993 1025 1263
Size: 473 32 488 32 238
Fragments arranged by size:
488 473 238 32 32
KpnI G_GTAC'C
Cuts at: 0 1224 1263
Size: 1224 39
MscI TGG'CCA
Cuts at: 0 430 538 1144 1263
Size: 430 108 606 119
Fragments arranged by size:
606 430 119 108
MspA1I CmG'CkG
Cuts at: 0 48 499 1023 1101 1263
Size: 48 451 524 78 162
Fragments arranged by size:
524 451 162 78 48
PasI CC'CwG_GG
Cuts at: 0 72 1263
Size: 72 1191
PflMI CCAn_nnn'nTGG
Cuts at: 0 48 534 1050 1263
Size: 48 486 516 213
Fragments arranged by size:
516 486 213 48
PfoI T'CCnGG_A
Cuts at: 0 80 470 839 986 1263
Size: 80 390 369 147 277
Fragments arranged by size:
390 369 277 147 80
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 906 938 1263
Size: 906 32 325
Fragments arranged by size:
906 325 32
PpuMI rG'GwC_Cy
Cuts at: 0 84 1263
Size: 84 1179
PstI C_TGCA'G
Cuts at: 0 464 1263
Size: 464 799
PvuII CAG'CTG
Cuts at: 0 1023 1101 1263
Size: 1023 78 162
Fragments arranged by size:
1023 162 78
SapI GCTCTTCn'nnn_
Cuts at: 0 796 1263
Size: 796 467
SfcI C'TryA_G
Cuts at: 0 460 1263
Size: 460 803
SmlI C'TyrA_G
Cuts at: 0 220 1092 1123 1263
Size: 220 872 31 140
Fragments arranged by size:
872 220 140 31
StyI C'CwwG_G
Cuts at: 0 419 477 1263
Size: 419 58 786
Fragments arranged by size:
786 419 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 802 944 1263
Size: 802 142 319
Fragments arranged by size:
802 319 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 865 897 1228 1260 1263
Size: 865 32 331 32 3
Fragments arranged by size:
865 331 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 0 298 1263
Size: 298 965
ZraI GAC'GTC
Cuts at: 0 606 1263
Size: 606 657
Enzymes that do cut:
AarI AatII Acc65I AcuI AlfI AloI AlwNI AvaI
BamHI BanI BanII BbsI BcgI BclI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstEII BstYI BtgI
BtgZI DraI DraIII EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRV FspI HaeII Hin4I KpnI MscI MspA1I
PasI PflMI PfoI PpiI PpuMI PstI PvuII SapI
SfcI SmlI StyI TaqII TstI Tth111I ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AleI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BciVI BfrBI BglII BmgBI BmtI BplI
BsaAI BsaBI BsiWI BsmI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DrdI EcoICRI EcoNI EcoRI FalI FseI FspAI HincII
HindIII HpaI KasI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XbaI XcmI XhoI XmaI XmnI