Restriction map for
pFN21AE3989
(Linear) (Six-base) MAPSORT of: KIEE3989.seq Check: 9735 from: 1 to: 1140
KIEE3989.orf
With 182 enzymes: SgfI *
July 3, 2012 18:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1140
Size: 508 632
AlwNI CAG_nnn'CTG
Cuts at: 0 447 736 1140
Size: 447 289 404
Fragments arranged by size:
447 404 289
ApoI r'AATT_y
Cuts at: 0 361 1140
Size: 361 779
BanI G'GyrC_C
Cuts at: 0 48 578 1140
Size: 48 530 562
Fragments arranged by size:
562 530 48
BanII G_rGCy'C
Cuts at: 0 743 1140
Size: 743 397
BbeI G_GCGC'C
Cuts at: 0 52 1140
Size: 52 1088
BbsI GAAGACnn'nnnn_
Cuts at: 0 947 1140
Size: 947 193
Bme1580I G_kGCm'C
Cuts at: 0 581 1140
Size: 581 559
BmrI ACTGGGnnnn_n'
Cuts at: 0 111 803 1022 1140
Size: 111 692 219 118
Fragments arranged by size:
692 219 118 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 499 1140
Size: 499 641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1140
Size: 55 1085
BsaBI GATnn'nnATC
Cuts at: 0 127 1140
Size: 127 1013
BsaHI Gr'CG_yC
Cuts at: 0 49 149 1090 1140
Size: 49 100 941 50
Fragments arranged by size:
941 100 50 49
BseYI C'CCAG_C
Cuts at: 0 117 279 1140
Size: 117 162 861
Fragments arranged by size:
861 162 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1140
Size: 707 433
BsiEI CG_ry'CG
Cuts at: 0 9 1140
Size: 9 1131
BsiHKAI G_wGCw'C
Cuts at: 0 743 1140
Size: 743 397
BsmI GAATG_Cn'
Cuts at: 0 555 1140
Size: 555 585
BsmBI CGTCTCn'nnnn_
Cuts at: 0 348 1140
Size: 348 792
Bsp1286I G_dGCh'C
Cuts at: 0 581 743 1140
Size: 581 162 397
Fragments arranged by size:
581 397 162
BspHI T'CATG_A
Cuts at: 0 642 1140
Size: 642 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 953 1140
Size: 953 187
BsrBI CCG'CTC
Cuts at: 0 849 1005 1140
Size: 849 156 135
Fragments arranged by size:
849 156 135
BsrFI r'CCGG_y
Cuts at: 0 1100 1140
Size: 1100 40
BssSI C'ACGA_G
Cuts at: 0 742 1140
Size: 742 398
BstBI TT'CG_AA
Cuts at: 0 266 1140
Size: 266 874
BstEII G'GTnAC_C
Cuts at: 0 397 1140
Size: 397 743
BstYI r'GATC_y
Cuts at: 0 93 122 1140
Size: 93 29 1018
Fragments arranged by size:
1018 93 29
BtgI C'CryG_G
Cuts at: 0 585 1140
Size: 585 555
BtsI GCAGTG_nn'
Cuts at: 0 69 1140
Size: 69 1071
DrdI GACnn_nn'nnGTC
Cuts at: 0 316 1140
Size: 316 824
EaeI y'GGCC_r
Cuts at: 0 615 1125 1140
Size: 615 510 15
Fragments arranged by size:
615 510 15
EcoICRI GAG'CTC
Cuts at: 0 741 1140
Size: 741 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 508 1140
Size: 55 453 632
Fragments arranged by size:
632 453 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 949 1140
Size: 949 191
HaeII r_GCGC'y
Cuts at: 0 52 1140
Size: 52 1088
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 1001 1033 1140
Size: 91 32 878 32 107
Fragments arranged by size:
878 107 91 32 32
KasI G'GCGC_C
Cuts at: 0 48 1140
Size: 48 1092
MscI TGG'CCA
Cuts at: 0 617 1140
Size: 617 523
MslI CAynn'nnrTG
Cuts at: 0 584 647 1140
Size: 584 63 493
Fragments arranged by size:
584 493 63
NarI GG'CG_CC
Cuts at: 0 49 1140
Size: 49 1091
NcoI C'CATG_G
Cuts at: 0 585 1140
Size: 585 555
PstI C_TGCA'G
Cuts at: 0 596 701 1140
Size: 596 105 439
Fragments arranged by size:
596 439 105
PvuI CG_AT'CG
Cuts at: 0 9 1140
Size: 9 1131
SacI G_AGCT'C
Cuts at: 0 743 1140
Size: 743 397
ScaI AGT'ACT
Cuts at: 0 493 1140
Size: 493 647
SfcI C'TryA_G
Cuts at: 0 592 603 697 1140
Size: 592 11 94 443
Fragments arranged by size:
592 443 94 11
SfoI GGC'GCC
Cuts at: 0 50 1140
Size: 50 1090
StuI AGG'CCT
Cuts at: 0 482 1140
Size: 482 658
StyI C'CwwG_G
Cuts at: 0 585 950 1140
Size: 585 365 190
Fragments arranged by size:
585 365 190
TatI w'GTAC_w
Cuts at: 0 462 491 1140
Size: 462 29 649
Fragments arranged by size:
649 462 29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 904 1140
Size: 312 592 236
Fragments arranged by size:
592 312 236
XmnI GAAnn'nnTTC
Cuts at: 0 493 1140
Size: 493 647
Enzymes that do cut:
AcuI AlwNI ApoI BanI BanII BbeI BbsI Bme1580I
BmrI BplI BpmI BsaBI BsaHI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstBI BstEII BstYI BtgI BtsI DrdI EaeI
EcoICRI Eco57MI EcoNI HaeII Hin4I KasI MscI MslI
NarI NcoI PstI PvuI SacI ScaI SfcI SfoI
StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BsiWI
BspEI BsrDI BsrGI BssHII BstAPI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EarI EciI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI ZraI