(Linear) (Six-base) MAPSORT of: KIEE3986.seq  Check: 1698  from: 1  to: 957



KIEE3986.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692     957 
   Size:        692     265
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     317     957 
   Size:        317     640
 
AlwNI CAG_nnn'CTG

Cuts at:      0      39     957 
   Size:         39     918
 
ApoI r'AATT_y

Cuts at:      0     646     859     957 
   Size:        646     213      98

  Fragments arranged by size: 

                 646     213      98
 
AseI AT'TA_AT

Cuts at:      0     206     957 
   Size:        206     751
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     164     198     957 
   Size:        164      34     759

  Fragments arranged by size: 

                 759     164      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     493     957 
   Size:        493     464
 
BlpI GC'TnA_GC

Cuts at:      0     232     957 
   Size:        232     725
 
Bpu10I CC'TnA_GC

Cuts at:      0     227     957 
   Size:        227     730
 
BsmI GAATG_Cn'

Cuts at:      0     264     957 
   Size:        264     693
 
BsrDI GCAATG_nn'

Cuts at:      0     601     957 
   Size:        601     356
 
BsrGI T'GTAC_A

Cuts at:      0     526     957 
   Size:        526     431
 
BstBI TT'CG_AA

Cuts at:      0     867     957 
   Size:        867      90
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      30     957 
   Size:         30     927
 
EaeI y'GGCC_r

Cuts at:      0     942     957 
   Size:        942      15
 
EarI CTCTTCn'nnn_

Cuts at:      0     668     957 
   Size:        668     289
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692     957 
   Size:        692     265
 
EcoO109I rG'GnC_Cy

Cuts at:      0     297     957 
   Size:        297     660
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     537     957 
   Size:        537     420
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     546     578     957 
   Size:        546      32     379

  Fragments arranged by size: 

                 546     379      32
 
HindIII A'AGCT_T

Cuts at:      0     234     957 
   Size:        234     723
 
MscI TGG'CCA

Cuts at:      0     944     957 
   Size:        944      13
 
MslI CAynn'nnrTG

Cuts at:      0     662     920     957 
   Size:        662     258      37

  Fragments arranged by size: 

                 662     258      37
 
MspA1I CmG'CkG

Cuts at:      0      39     414     957 
   Size:         39     375     543

  Fragments arranged by size: 

                 543     375      39
 
PfoI T'CCnGG_A

Cuts at:      0     485     957 
   Size:        485     472
 
PpuMI rG'GwC_Cy

Cuts at:      0     297     957 
   Size:        297     660
 
PsiI TTA'TAA

Cuts at:      0     703     957 
   Size:        703     254
 
PvuII CAG'CTG

Cuts at:      0      39     414     957 
   Size:         39     375     543

  Fragments arranged by size: 

                 543     375      39
 
SapI GCTCTTCn'nnn_

Cuts at:      0     668     957 
   Size:        668     289
 
SfcI C'TryA_G

Cuts at:      0      54     957 
   Size:         54     903
 
SspI AAT'ATT

Cuts at:      0     894     957 
   Size:        894      63
 
StyI C'CwwG_G

Cuts at:      0     141     476     957 
   Size:        141     335     481

  Fragments arranged by size: 

                 481     335     141
 
TatI w'GTAC_w

Cuts at:      0     526     783     957 
   Size:        526     257     174

  Fragments arranged by size: 

                 526     257     174
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     483     957 
   Size:        483     474
 
XmnI GAAnn'nnTTC

Cuts at:      0     650     863     957 
   Size:        650     213      94

  Fragments arranged by size: 

                 650     213      94
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApoI     AseI     BcgI    BciVI     BlpI
   Bpu10I     BsmI    BsrDI    BsrGI    BstBI    CspCI     EaeI     EarI
  Eco57MI EcoO109I     FalI    Hin4I  HindIII     MscI     MslI   MspA1I
     PfoI    PpuMI     PsiI    PvuII     SapI     SfcI     SspI     StyI
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI