(Linear) (Six-base) MAPSORT of: KIEE3985.seq  Check: 2752  from: 1  to: 1167



KIEE3985.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     657    1167 
   Size:        657     510
 
AflIII A'CryG_T

Cuts at:      0     353    1148    1167 
   Size:        353     795      19

  Fragments arranged by size: 

                 795     353      19
 
AleI CACnn'nnGTG

Cuts at:      0     121     319    1167 
   Size:        121     198     848

  Fragments arranged by size: 

                 848     198     121
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     780    1167 
   Size:        780     387
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1042    1167 
   Size:       1042     125
 
ApoI r'AATT_y

Cuts at:      0     567     610    1167 
   Size:        567      43     557

  Fragments arranged by size: 

                 567     557      43
 
AseI AT'TA_AT

Cuts at:      0     488    1167 
   Size:        488     679
 
BamHI G'GATC_C

Cuts at:      0     888    1167 
   Size:        888     279
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      84     118    1167 
   Size:         84      34    1049

  Fragments arranged by size: 

                1049      84      34
 
BclI T'GATC_A

Cuts at:      0     164    1167 
   Size:        164    1003
 
BmtI G_CTAG'C

Cuts at:      0     631    1167 
   Size:        631     536
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     808    1167 
   Size:        808     359
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     808    1167 
   Size:        808     359
 
BsaWI w'CCGG_w

Cuts at:      0     579    1167 
   Size:        579     588
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     413    1037    1167 
   Size:        413     624     130

  Fragments arranged by size: 

                 624     413     130
 
BsiHKAI G_wGCw'C

Cuts at:      0     316    1167 
   Size:        316     851
 
Bsp1286I G_dGCh'C

Cuts at:      0     316    1167 
   Size:        316     851
 
BstBI TT'CG_AA

Cuts at:      0     434    1167 
   Size:        434     733
 
BstEII G'GTnAC_C

Cuts at:      0       9    1167 
   Size:          9    1158
 
BstYI r'GATC_y

Cuts at:      0     888    1167 
   Size:        888     279
 
Bsu36I CC'TnA_GG

Cuts at:      0     239    1167 
   Size:        239     928
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     669    1167 
   Size:        669     498
 
DraIII CAC_nnn'GTG

Cuts at:      0    1159    1167 
   Size:       1159       8
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     370    1167 
   Size:        370     797
 
EaeI y'GGCC_r

Cuts at:      0      89     725    1167 
   Size:         89     636     442

  Fragments arranged by size: 

                 636     442      89
 
EarI CTCTTCn'nnn_

Cuts at:      0    1121    1167 
   Size:       1121      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     657     808    1167 
   Size:        657     151     359

  Fragments arranged by size: 

                 657     359     151
 
EcoRI G'AATT_C

Cuts at:      0     567    1167 
   Size:        567     600
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     661    1127    1167 
   Size:        264     397     466      40

  Fragments arranged by size: 

                 466     397     264      40
 
MscI TGG'CCA

Cuts at:      0      91     727    1167 
   Size:         91     636     440

  Fragments arranged by size: 

                 636     440      91
 
MslI CAynn'nnrTG

Cuts at:      0     121     319    1167 
   Size:        121     198     848

  Fragments arranged by size: 

                 848     198     121
 
MspA1I CmG'CkG

Cuts at:      0     740    1167 
   Size:        740     427
 
NheI G'CTAG_C

Cuts at:      0     627    1167 
   Size:        627     540
 
NspI r_CATG'y

Cuts at:      0     357     757    1152    1167 
   Size:        357     400     395      15

  Fragments arranged by size: 

                 400     395     357      15
 
PciI A'CATG_T

Cuts at:      0     353    1148    1167 
   Size:        353     795      19

  Fragments arranged by size: 

                 795     353      19
 
PshAI GACnn'nnGTC

Cuts at:      0     419    1167 
   Size:        419     748
 
ScaI AGT'ACT

Cuts at:      0     430    1167 
   Size:        430     737
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     431     487    1058    1167 
   Size:        431      56     571     109

  Fragments arranged by size: 

                 571     431     109      56
 
TatI w'GTAC_w

Cuts at:      0     428    1167 
   Size:        428     739
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI     AseI    BamHI
     BcgI     BclI     BmtI     BplI     BpmI    BsaWI    BseRI  BsiHKAI
 Bsp1286I    BstBI   BstEII    BstYI   Bsu36I    BtgZI   DraIII     DrdI
     EaeI     EarI  Eco57MI    EcoRI     MmeI     MscI     MslI   MspA1I
     NheI     NspI     PciI    PshAI     ScaI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstXI  BstZ17I     BtgI
     BtsI     ClaI    CspCI     DraI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI