Restriction map for
pFN21AE3983
(Linear) (Six-base) MAPSORT of: KIEE3983.seq Check: 3985 from: 1 to: 711
KIEE3983.orf
With 182 enzymes: SgfI *
March 22, 2012 17:03 ..
AccI GT'mk_AC
Cuts at: 0 67 711
Size: 67 644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 711
Size: 206 505
AflII C'TTAA_G
Cuts at: 0 213 711
Size: 213 498
AflIII A'CryG_T
Cuts at: 0 91 711
Size: 91 620
AleI CACnn'nnGTG
Cuts at: 0 625 638 711
Size: 625 13 73
Fragments arranged by size:
625 73 13
AlwNI CAG_nnn'CTG
Cuts at: 0 409 515 711
Size: 409 106 196
Fragments arranged by size:
409 196 106
ApoI r'AATT_y
Cuts at: 0 253 711
Size: 253 458
AvaI C'yCGr_G
Cuts at: 0 146 623 711
Size: 146 477 88
Fragments arranged by size:
477 146 88
BanII G_rGCy'C
Cuts at: 0 299 711
Size: 299 412
BbsI GAAGACnn'nnnn_
Cuts at: 0 706 711
Size: 706 5
BciVI GTATCCnnnnn_n'
Cuts at: 0 336 344 711
Size: 336 8 367
Fragments arranged by size:
367 336 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 24 31 397 711
Size: 24 7 366 314
Fragments arranged by size:
366 314 24 7
BmtI G_CTAG'C
Cuts at: 0 694 711
Size: 694 17
Bpu10I CC'TnA_GC
Cuts at: 0 323 711
Size: 323 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 711
Size: 140 571
BsaI GGTCTCn'nnnn_
Cuts at: 0 143 711
Size: 143 568
BsiHKAI G_wGCw'C
Cuts at: 0 671 711
Size: 671 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 80 711
Size: 80 631
Bsp1286I G_dGCh'C
Cuts at: 0 299 671 711
Size: 299 372 40
Fragments arranged by size:
372 299 40
BsrBI CCG'CTC
Cuts at: 0 645 711
Size: 645 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 711
Size: 405 306
BstZ17I GTA'TAC
Cuts at: 0 68 711
Size: 68 643
BtgI C'CryG_G
Cuts at: 0 338 711
Size: 338 373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 711
Size: 206 505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 583 711
Size: 583 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 188 220 294 326 711
Size: 188 32 74 32 385
Fragments arranged by size:
385 188 74 32 32
HindIII A'AGCT_T
Cuts at: 0 382 711
Size: 382 329
MluI A'CGCG_T
Cuts at: 0 91 711
Size: 91 620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 422 711
Size: 221 201 289
Fragments arranged by size:
289 221 201
MslI CAynn'nnrTG
Cuts at: 0 625 638 711
Size: 625 13 73
Fragments arranged by size:
625 73 13
NcoI C'CATG_G
Cuts at: 0 338 711
Size: 338 373
NheI G'CTAG_C
Cuts at: 0 690 711
Size: 690 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 582 711
Size: 582 129
ScaI AGT'ACT
Cuts at: 0 375 394 711
Size: 375 19 317
Fragments arranged by size:
375 317 19
SfcI C'TryA_G
Cuts at: 0 684 711
Size: 684 27
SmaI CCC'GGG
Cuts at: 0 625 711
Size: 625 86
SmlI C'TyrA_G
Cuts at: 0 146 155 213 711
Size: 146 9 58 498
Fragments arranged by size:
498 146 58 9
StyI C'CwwG_G
Cuts at: 0 338 711
Size: 338 373
TatI w'GTAC_w
Cuts at: 0 373 392 711
Size: 373 19 319
Fragments arranged by size:
373 319 19
Tth111I GACn'n_nGTC
Cuts at: 0 37 711
Size: 37 674
XhoI C'TCGA_G
Cuts at: 0 146 711
Size: 146 565
XmaI C'CCGG_G
Cuts at: 0 623 711
Size: 623 88
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApoI AvaI
BanII BbsI BciVI BmrI BmtI Bpu10I BpuEI BsaI
BsiHKAI BsmBI Bsp1286I BsrBI BstXI BstZ17I BtgI Eco57MI
FalI Hin4I HindIII MluI MmeI MslI NcoI NheI
PflMI ScaI SfcI SmaI SmlI StyI TatI Tth111I
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BplI BpmI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MscI
MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI XbaI XcmI
XmnI ZraI