Restriction map for
pFN21AE3982
(Linear) (Six-base) MAPSORT of: KIEE3982.seq Check: 7170 from: 1 to: 987
KIEE3982.orf
With 182 enzymes: SgfI *
March 22, 2012 17:03 ..
AccI GT'mk_AC
Cuts at: 0 50 987
Size: 50 937
AclI AA'CG_TT
Cuts at: 0 958 987
Size: 958 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 987
Size: 985 2
ApoI r'AATT_y
Cuts at: 0 169 590 760 969 987
Size: 169 421 170 209 18
Fragments arranged by size:
421 209 170 169 18
BanI G'GyrC_C
Cuts at: 0 475 987
Size: 475 512
BbsI GAAGACnn'nnnn_
Cuts at: 0 493 987
Size: 493 494
BciVI GTATCCnnnnn_n'
Cuts at: 0 693 910 987
Size: 693 217 77
Fragments arranged by size:
693 217 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 511 931 987
Size: 487 24 420 56
Fragments arranged by size:
487 420 56 24
BsaAI yAC'GTr
Cuts at: 0 345 987
Size: 345 642
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 329 359 368 398 415 445 987
Size: 329 30 9 30 17 30 542
Fragments arranged by size:
542 329 30 30 30 17 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 599 987
Size: 599 388
BseYI C'CCAG_C
Cuts at: 0 485 987
Size: 485 502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 987
Size: 473 514
BsiEI CG_ry'CG
Cuts at: 0 25 987
Size: 25 962
BsrDI GCAATG_nn'
Cuts at: 0 316 642 987
Size: 316 326 345
Fragments arranged by size:
345 326 316
BstEII G'GTnAC_C
Cuts at: 0 535 987
Size: 535 452
ClaI AT'CG_AT
Cuts at: 0 210 987
Size: 210 777
EaeI y'GGCC_r
Cuts at: 0 22 987
Size: 22 965
EagI C'GGCC_G
Cuts at: 0 22 987
Size: 22 965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 511 931 985 987
Size: 487 24 420 54 2
Fragments arranged by size:
487 420 54 24 2
EcoRV GAT'ATC
Cuts at: 0 563 774 987
Size: 563 211 213
Fragments arranged by size:
563 213 211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 262 349 987
Size: 262 87 638
Fragments arranged by size:
638 262 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 758 790 884 916 987
Size: 758 32 94 32 71
Fragments arranged by size:
758 94 71 32 32
HincII GTy'rAC
Cuts at: 0 582 987
Size: 582 405
HindIII A'AGCT_T
Cuts at: 0 82 655 987
Size: 82 573 332
Fragments arranged by size:
573 332 82
MspA1I CmG'CkG
Cuts at: 0 166 489 951 987
Size: 166 323 462 36
Fragments arranged by size:
462 323 166 36
NspI r_CATG'y
Cuts at: 0 353 431 715 987
Size: 353 78 284 272
Fragments arranged by size:
353 284 272 78
PmlI CAC'GTG
Cuts at: 0 345 987
Size: 345 642
PsiI TTA'TAA
Cuts at: 0 281 987
Size: 281 706
PvuII CAG'CTG
Cuts at: 0 166 489 951 987
Size: 166 323 462 36
Fragments arranged by size:
462 323 166 36
SfcI C'TryA_G
Cuts at: 0 806 987
Size: 806 181
SphI G_CATG'C
Cuts at: 0 431 987
Size: 431 556
SspI AAT'ATT
Cuts at: 0 694 845 987
Size: 694 151 142
Fragments arranged by size:
694 151 142
StyI C'CwwG_G
Cuts at: 0 186 333 435 987
Size: 186 147 102 552
Fragments arranged by size:
552 186 147 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 329 361 623 655 987
Size: 329 32 262 32 332
Fragments arranged by size:
332 329 262 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 307 987
Size: 307 680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 486 987
Size: 363 123 501
Fragments arranged by size:
501 363 123
XmnI GAAnn'nnTTC
Cuts at: 0 970 987
Size: 970 17
Enzymes that do cut:
AccI AclI AcuI ApoI BanI BbsI BciVI BpmI
BsaAI BsaXI BseRI BseYI BsgI BsiEI BsrDI BstEII
ClaI EaeI EagI Eco57MI EcoRV FalI Hin4I HincII
HindIII MspA1I NspI PmlI PsiI PvuII SfcI SphI
SspI StyI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoO109I
EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI
TaqII TatI XbaI XhoI XmaI ZraI