Restriction map for
pFN21AE3981
(Linear) (Six-base) MAPSORT of: KIEE3981.seq Check: 487 from: 1 to: 771
KIEE3981.orf
With 182 enzymes: SgfI *
March 22, 2012 17:03 ..
AccI GT'mk_AC
Cuts at: 0 674 771
Size: 674 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 771
Size: 334 437
AflIII A'CryG_T
Cuts at: 0 116 771
Size: 116 655
ApoI r'AATT_y
Cuts at: 0 264 771
Size: 264 507
AvaI C'yCGr_G
Cuts at: 0 619 771
Size: 619 152
BanII G_rGCy'C
Cuts at: 0 194 658 771
Size: 194 464 113
Fragments arranged by size:
464 194 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 177 771
Size: 177 594
BclI T'GATC_A
Cuts at: 0 14 771
Size: 14 757
BlpI GC'TnA_GC
Cuts at: 0 469 771
Size: 469 302
BmgBI CAC'GTC
Cuts at: 0 519 567 771
Size: 519 48 204
Fragments arranged by size:
519 204 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 678 771
Size: 678 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 771
Size: 47 724
BsaHI Gr'CG_yC
Cuts at: 0 724 771
Size: 724 47
BsaWI w'CCGG_w
Cuts at: 0 746 771
Size: 746 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 683 771
Size: 277 406 88
Fragments arranged by size:
406 277 88
Bsp1286I G_dGCh'C
Cuts at: 0 194 658 771
Size: 194 464 113
Fragments arranged by size:
464 194 113
BspEI T'CCGG_A
Cuts at: 0 746 771
Size: 746 25
BsrGI T'GTAC_A
Cuts at: 0 119 771
Size: 119 652
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 201 363 771
Size: 132 69 162 408
Fragments arranged by size:
408 162 132 69
BstYI r'GATC_y
Cuts at: 0 22 771
Size: 22 749
EaeI y'GGCC_r
Cuts at: 0 111 134 771
Size: 111 23 637
Fragments arranged by size:
637 111 23
EarI CTCTTCn'nnn_
Cuts at: 0 323 771
Size: 323 448
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 735 771
Size: 735 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 334 771
Size: 47 287 437
Fragments arranged by size:
437 287 47
EcoO109I rG'GnC_Cy
Cuts at: 0 413 615 771
Size: 413 202 156
Fragments arranged by size:
413 202 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 446 478 632 664 771
Size: 31 415 32 154 32 107
Fragments arranged by size:
415 154 107 32 32 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 771
Size: 344 427
MscI TGG'CCA
Cuts at: 0 136 771
Size: 136 635
MslI CAynn'nnrTG
Cuts at: 0 101 103 771
Size: 101 2 668
Fragments arranged by size:
668 101 2
NspI r_CATG'y
Cuts at: 0 120 771
Size: 120 651
PciI A'CATG_T
Cuts at: 0 116 771
Size: 116 655
PflMI CCAn_nnn'nTGG
Cuts at: 0 586 771
Size: 586 185
PpuMI rG'GwC_Cy
Cuts at: 0 615 771
Size: 615 156
PshAI GACnn'nnGTC
Cuts at: 0 565 771
Size: 565 206
SmlI C'TyrA_G
Cuts at: 0 619 771
Size: 619 152
SspI AAT'ATT
Cuts at: 0 340 771
Size: 340 431
StyI C'CwwG_G
Cuts at: 0 48 771
Size: 48 723
TatI w'GTAC_w
Cuts at: 0 119 771
Size: 119 652
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 714 746 771
Size: 714 32 25
Fragments arranged by size:
714 32 25
XhoI C'TCGA_G
Cuts at: 0 619 771
Size: 619 152
XmnI GAAnn'nnTTC
Cuts at: 0 500 771
Size: 500 271
Enzymes that do cut:
AccI AcuI AflIII ApoI AvaI BanII BbsI BclI
BlpI BmgBI BmrI BpmI BsaHI BsaWI BseRI Bsp1286I
BspEI BsrGI BstXI BstYI EaeI EarI EciI Eco57MI
EcoO109I Hin4I MmeI MscI MslI NspI PciI PflMI
PpuMI PshAI SmlI SspI StyI TatI TstI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI
XcmI XmaI ZraI