Restriction map for
pFN21AE3975
(Linear) (Six-base) MAPSORT of: KIEE3975.seq Check: 8647 from: 1 to: 741
KIEE3975.orf
With 182 enzymes: SgfI *
March 22, 2012 17:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 741
Size: 402 339
AlwNI CAG_nnn'CTG
Cuts at: 0 246 741
Size: 246 495
ApoI r'AATT_y
Cuts at: 0 337 741
Size: 337 404
AvaI C'yCGr_G
Cuts at: 0 350 741
Size: 350 391
BanI G'GyrC_C
Cuts at: 0 297 741
Size: 297 444
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 741
Size: 145 596
BbvCI CC'TCA_GC
Cuts at: 0 557 608 741
Size: 557 51 133
Fragments arranged by size:
557 133 51
BclI T'GATC_A
Cuts at: 0 648 741
Size: 648 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 389 741
Size: 389 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 502 741
Size: 4 498 239
Fragments arranged by size:
498 239 4
Bpu10I CC'TnA_GC
Cuts at: 0 557 608 741
Size: 557 51 133
Fragments arranged by size:
557 133 51
BsaWI w'CCGG_w
Cuts at: 0 572 741
Size: 572 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 554 584 741
Size: 554 30 157
Fragments arranged by size:
554 157 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 73 741
Size: 70 3 668
Fragments arranged by size:
668 70 3
BseYI C'CCAG_C
Cuts at: 0 371 741
Size: 371 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 741
Size: 637 104
BspEI T'CCGG_A
Cuts at: 0 572 741
Size: 572 169
BsrBI CCG'CTC
Cuts at: 0 486 741
Size: 486 255
BsrFI r'CCGG_y
Cuts at: 0 115 741
Size: 115 626
BsrGI T'GTAC_A
Cuts at: 0 29 741
Size: 29 712
BssSI C'ACGA_G
Cuts at: 0 232 741
Size: 232 509
BstAPI GCAn_nnn'nTGC
Cuts at: 0 206 741
Size: 206 535
BstEII G'GTnAC_C
Cuts at: 0 366 741
Size: 366 375
BstXI CCAn_nnnn'nTGG
Cuts at: 0 379 741
Size: 379 362
BstYI r'GATC_y
Cuts at: 0 227 741
Size: 227 514
BtsI GCAGTG_nn'
Cuts at: 0 244 741
Size: 244 497
DraIII CAC_nnn'GTG
Cuts at: 0 149 741
Size: 149 592
EarI CTCTTCn'nnn_
Cuts at: 0 314 380 727 741
Size: 314 66 347 14
Fragments arranged by size:
347 314 66 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 402 502 741
Size: 4 398 100 239
Fragments arranged by size:
398 239 100 4
EcoO109I rG'GnC_Cy
Cuts at: 0 579 741
Size: 579 162
EcoRI G'AATT_C
Cuts at: 0 337 741
Size: 337 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 59 91 741
Size: 59 32 650
Fragments arranged by size:
650 59 32
MslI CAynn'nnrTG
Cuts at: 0 658 741
Size: 658 83
MspA1I CmG'CkG
Cuts at: 0 683 741
Size: 683 58
NaeI GCC'GGC
Cuts at: 0 117 741
Size: 117 624
NgoMIV G'CCGG_C
Cuts at: 0 115 741
Size: 115 626
NspI r_CATG'y
Cuts at: 0 616 741
Size: 616 125
PpuMI rG'GwC_Cy
Cuts at: 0 579 741
Size: 579 162
PstI C_TGCA'G
Cuts at: 0 527 741
Size: 527 214
SapI GCTCTTCn'nnn_
Cuts at: 0 380 741
Size: 380 361
ScaI AGT'ACT
Cuts at: 0 205 741
Size: 205 536
SfcI C'TryA_G
Cuts at: 0 523 741
Size: 523 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 741
Size: 385 356
TatI w'GTAC_w
Cuts at: 0 29 203 741
Size: 29 174 538
Fragments arranged by size:
538 174 29
Tth111I GACn'n_nGTC
Cuts at: 0 268 364 741
Size: 268 96 377
Fragments arranged by size:
377 268 96
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanI BbsI BbvCI BclI
BmrI BpmI Bpu10I BsaWI BsaXI BseRI BseYI BsgI
BspEI BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI BtsI DraIII EarI Eco57MI EcoO109I EcoRI Hin4I
MslI MspA1I NaeI NgoMIV NspI PpuMI PstI SapI
ScaI SfcI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BssHII
BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TstI XbaI XcmI XhoI XmaI XmnI ZraI