Restriction map for
pFN21AE3973
(Linear) (Six-base) MAPSORT of: KIEE3973.seq Check: 5770 from: 1 to: 1302
KIEE3973.orf
With 182 enzymes: SgfI *
July 3, 2012 18:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 150 1302
Size: 150 1152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1302
Size: 40 1262
AflIII A'CryG_T
Cuts at: 0 927 1302
Size: 927 375
AleI CACnn'nnGTG
Cuts at: 0 857 1302
Size: 857 445
AlwNI CAG_nnn'CTG
Cuts at: 0 445 1302
Size: 445 857
ApaLI G'TGCA_C
Cuts at: 0 1195 1302
Size: 1195 107
ApoI r'AATT_y
Cuts at: 0 160 1302
Size: 160 1142
AvaI C'yCGr_G
Cuts at: 0 811 1302
Size: 811 491
Bme1580I G_kGCm'C
Cuts at: 0 1199 1302
Size: 1199 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 1216 1302
Size: 1216 86
BsaAI yAC'GTr
Cuts at: 0 1200 1302
Size: 1200 102
BsaBI GATnn'nnATC
Cuts at: 0 118 1302
Size: 118 1184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 542 572 1195 1225 1302
Size: 542 30 623 30 77
Fragments arranged by size:
623 542 77 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 756 1302
Size: 756 546
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 808 835 1302
Size: 637 171 27 467
Fragments arranged by size:
637 467 171 27
BsiHKAI G_wGCw'C
Cuts at: 0 748 1199 1302
Size: 748 451 103
Fragments arranged by size:
748 451 103
BsmI GAATG_Cn'
Cuts at: 0 567 1302
Size: 567 735
Bsp1286I G_dGCh'C
Cuts at: 0 748 1199 1302
Size: 748 451 103
Fragments arranged by size:
748 451 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 150 1302
Size: 150 1152
BssSI C'ACGA_G
Cuts at: 0 999 1302
Size: 999 303
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1288 1302
Size: 1288 14
BstYI r'GATC_y
Cuts at: 0 641 1302
Size: 641 661
DraI TTT'AAA
Cuts at: 0 953 1302
Size: 953 349
DraIII CAC_nnn'GTG
Cuts at: 0 735 1302
Size: 735 567
EaeI y'GGCC_r
Cuts at: 0 469 1302
Size: 469 833
EarI CTCTTCn'nnn_
Cuts at: 0 901 1185 1302
Size: 901 284 117
Fragments arranged by size:
901 284 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1302
Size: 40 1262
EcoNI CCTnn'n_nnAGG
Cuts at: 0 311 1302
Size: 311 991
EcoO109I rG'GnC_Cy
Cuts at: 0 339 1302
Size: 339 963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1252 1302
Size: 1252 50
HaeII r_GCGC'y
Cuts at: 0 81 1302
Size: 81 1221
HincII GTy'rAC
Cuts at: 0 303 491 1245 1302
Size: 303 188 754 57
Fragments arranged by size:
754 303 188 57
MscI TGG'CCA
Cuts at: 0 471 1302
Size: 471 831
MslI CAynn'nnrTG
Cuts at: 0 857 1302
Size: 857 445
MspA1I CmG'CkG
Cuts at: 0 857 1302
Size: 857 445
NspI r_CATG'y
Cuts at: 0 415 931 1302
Size: 415 516 371
Fragments arranged by size:
516 415 371
PciI A'CATG_T
Cuts at: 0 927 1302
Size: 927 375
PmlI CAC'GTG
Cuts at: 0 1200 1302
Size: 1200 102
SexAI A'CCwGG_T
Cuts at: 0 1247 1302
Size: 1247 55
SspI AAT'ATT
Cuts at: 0 350 915 1302
Size: 350 565 387
Fragments arranged by size:
565 387 350
StuI AGG'CCT
Cuts at: 0 317 1302
Size: 317 985
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1240 1302
Size: 1240 62
TatI w'GTAC_w
Cuts at: 0 58 584 1302
Size: 58 526 718
Fragments arranged by size:
718 526 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 303 335 1195 1227 1302
Size: 303 32 860 32 75
Fragments arranged by size:
860 303 75 32 32
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaLI ApoI AvaI
Bme1580I BsaI BsaAI BsaBI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspMI BssSI BstXI BstYI DraI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII HincII
MscI MslI MspA1I NspI PciI PmlI SexAI SspI
StuI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaHI BsaWI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DrdI EagI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StyI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI