Restriction map for
pFN21AE3972
(Linear) (Six-base) MAPSORT of: KIEE3972.seq Check: 5307 from: 1 to: 1308
KIEE3972.orf
With 182 enzymes: SgfI *
March 22, 2012 17:01 ..
AfeI AGC'GCT
Cuts at: 0 1282 1308
Size: 1282 26
AflIII A'CryG_T
Cuts at: 0 400 473 1308
Size: 400 73 835
Fragments arranged by size:
835 400 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 360 1308
Size: 360 948
AlwNI CAG_nnn'CTG
Cuts at: 0 161 191 1295 1308
Size: 161 30 1104 13
Fragments arranged by size:
1104 161 30 13
ApaLI G'TGCA_C
Cuts at: 0 650 1308
Size: 650 658
ApoI r'AATT_y
Cuts at: 0 1025 1308
Size: 1025 283
AseI AT'TA_AT
Cuts at: 0 949 1308
Size: 949 359
BanI G'GyrC_C
Cuts at: 0 437 734 1308
Size: 437 297 574
Fragments arranged by size:
574 437 297
BbsI GAAGACnn'nnnn_
Cuts at: 0 283 1308
Size: 283 1025
BclI T'GATC_A
Cuts at: 0 1010 1308
Size: 1010 298
BfrBI ATG'CAT
Cuts at: 0 322 1108 1308
Size: 322 786 200
Fragments arranged by size:
786 322 200
Bme1580I G_kGCm'C
Cuts at: 0 654 1308
Size: 654 654
BmgBI CAC'GTC
Cuts at: 0 12 1308
Size: 12 1296
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 1013 1308
Size: 63 950 295
Fragments arranged by size:
950 295 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 1087 1135 1308
Size: 138 949 48 173
Fragments arranged by size:
949 173 138 48
Bpu10I CC'TnA_GC
Cuts at: 0 146 412 1308
Size: 146 266 896
Fragments arranged by size:
896 266 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 622 1162 1308
Size: 574 48 540 146
Fragments arranged by size:
574 540 146 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 592 718 1308
Size: 156 436 126 590
Fragments arranged by size:
590 436 156 126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 1308
Size: 18 1290
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 1308
Size: 597 711
BsiHKAI G_wGCw'C
Cuts at: 0 147 654 1121 1308
Size: 147 507 467 187
Fragments arranged by size:
507 467 187 147
BsmI GAATG_Cn'
Cuts at: 0 557 801 1308
Size: 557 244 507
Fragments arranged by size:
557 507 244
Bsp1286I G_dGCh'C
Cuts at: 0 147 654 1121 1308
Size: 147 507 467 187
Fragments arranged by size:
507 467 187 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 1308
Size: 23 1285
BsrBI CCG'CTC
Cuts at: 0 1146 1308
Size: 1146 162
BsrGI T'GTAC_A
Cuts at: 0 379 457 1308
Size: 379 78 851
Fragments arranged by size:
851 379 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 734 1295 1308
Size: 734 561 13
Fragments arranged by size:
734 561 13
BtgI C'CryG_G
Cuts at: 0 817 1308
Size: 817 491
BtsI GCAGTG_nn'
Cuts at: 0 654 1308
Size: 654 654
EarI CTCTTCn'nnn_
Cuts at: 0 914 1120 1308
Size: 914 206 188
Fragments arranged by size:
914 206 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 1087 1135 1308
Size: 138 949 48 173
Fragments arranged by size:
949 173 138 48
EcoO109I rG'GnC_Cy
Cuts at: 0 158 1046 1202 1308
Size: 158 888 156 106
Fragments arranged by size:
888 158 156 106
EcoRI G'AATT_C
Cuts at: 0 1025 1308
Size: 1025 283
HaeII r_GCGC'y
Cuts at: 0 1284 1308
Size: 1284 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1283 1308
Size: 1283 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 797 978 1308
Size: 491 306 181 330
Fragments arranged by size:
491 330 306 181
MspA1I CmG'CkG
Cuts at: 0 140 1065 1308
Size: 140 925 243
Fragments arranged by size:
925 243 140
NsiI A_TGCA'T
Cuts at: 0 324 1110 1308
Size: 324 786 198
Fragments arranged by size:
786 324 198
NspI r_CATG'y
Cuts at: 0 404 477 1308
Size: 404 73 831
Fragments arranged by size:
831 404 73
PciI A'CATG_T
Cuts at: 0 400 473 1308
Size: 400 73 835
Fragments arranged by size:
835 400 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 161 1308
Size: 161 1147
PpuMI rG'GwC_Cy
Cuts at: 0 1202 1308
Size: 1202 106
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 83 115 1308
Size: 83 32 1193
Fragments arranged by size:
1193 83 32
PstI C_TGCA'G
Cuts at: 0 349 1291 1308
Size: 349 942 17
Fragments arranged by size:
942 349 17
PvuII CAG'CTG
Cuts at: 0 1065 1308
Size: 1065 243
SapI GCTCTTCn'nnn_
Cuts at: 0 1120 1308
Size: 1120 188
ScaI AGT'ACT
Cuts at: 0 362 1308
Size: 362 946
SfcI C'TryA_G
Cuts at: 0 345 1287 1308
Size: 345 942 21
Fragments arranged by size:
942 345 21
SmlI C'TyrA_G
Cuts at: 0 589 637 1141 1308
Size: 589 48 504 167
Fragments arranged by size:
589 504 167 48
SpeI A'CTAG_T
Cuts at: 0 567 1308
Size: 567 741
SspI AAT'ATT
Cuts at: 0 252 830 1218 1308
Size: 252 578 388 90
Fragments arranged by size:
578 388 252 90
StuI AGG'CCT
Cuts at: 0 595 1308
Size: 595 713
StyI C'CwwG_G
Cuts at: 0 548 1308
Size: 548 760
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 365 1308
Size: 365 943
TatI w'GTAC_w
Cuts at: 0 360 379 403 457 641 1308
Size: 360 19 24 54 184 667
Fragments arranged by size:
667 360 184 54 24 19
XbaI T'CTAG_A
Cuts at: 0 117 1308
Size: 117 1191
Enzymes that do cut:
AfeI AflIII AlfI AlwNI ApaLI ApoI AseI BanI
BbsI BclI BfrBI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrGI BstAPI BtgI BtsI EarI Eco57MI EcoO109I
EcoRI HaeII Hin4I MmeI MspA1I NsiI NspI PciI
PflMI PpuMI PsrI PstI PvuII SapI ScaI SfcI
SmlI SpeI SspI StuI StyI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BglI
BglII BlpI BmrI BmtI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI