Restriction map for
pFN21AE3968
(Linear) (Six-base) MAPSORT of: KIEE3968.seq Check: 6175 from: 1 to: 1215
KIEE3968.orf
With 182 enzymes: SgfI *
July 3, 2012 18:22 ..
AccI GT'mk_AC
Cuts at: 0 782 1215
Size: 782 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 850 856 1145 1215
Size: 528 322 6 289 70
Fragments arranged by size:
528 322 289 70 6
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 1215
Size: 325 890
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 754 786 1215
Size: 754 32 429
Fragments arranged by size:
754 429 32
AlwNI CAG_nnn'CTG
Cuts at: 0 551 1155 1215
Size: 551 604 60
Fragments arranged by size:
604 551 60
AvaI C'yCGr_G
Cuts at: 0 362 697 1215
Size: 362 335 518
Fragments arranged by size:
518 362 335
BamHI G'GATC_C
Cuts at: 0 383 1215
Size: 383 832
BanI G'GyrC_C
Cuts at: 0 1063 1215
Size: 1063 152
BanII G_rGCy'C
Cuts at: 0 149 1007 1141 1184 1215
Size: 149 858 134 43 31
Fragments arranged by size:
858 149 134 43 31
BbeI G_GCGC'C
Cuts at: 0 1067 1215
Size: 1067 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 586 1215
Size: 586 629
BbvCI CC'TCA_GC
Cuts at: 0 205 1215
Size: 205 1010
BclI T'GATC_A
Cuts at: 0 92 1215
Size: 92 1123
BglI GCCn_nnn'nGGC
Cuts at: 0 985 1215
Size: 985 230
Bme1580I G_kGCm'C
Cuts at: 0 231 314 1168 1215
Size: 231 83 854 47
Fragments arranged by size:
854 231 83 47
BmrI ACTGGGnnnn_n'
Cuts at: 0 962 1048 1215
Size: 962 86 167
Fragments arranged by size:
962 167 86
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 708 1215
Size: 708 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 494 823 865 898 955 1215
Size: 148 346 329 42 33 57 260
Fragments arranged by size:
346 329 260 148 57 42 33
Bpu10I CC'TnA_GC
Cuts at: 0 205 1215
Size: 205 1010
BsaHI Gr'CG_yC
Cuts at: 0 1064 1215
Size: 1064 151
BsaWI w'CCGG_w
Cuts at: 0 511 1085 1215
Size: 511 574 130
Fragments arranged by size:
574 511 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 754 784 1102 1132 1215
Size: 754 30 318 30 83
Fragments arranged by size:
754 318 83 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 664 667 820 895 1192 1215
Size: 181 483 3 153 75 297 23
Fragments arranged by size:
483 297 181 153 75 23 3
BseYI C'CCAG_C
Cuts at: 0 231 1148 1215
Size: 231 917 67
Fragments arranged by size:
917 231 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1215
Size: 123 1092
BsiHKAI G_wGCw'C
Cuts at: 0 704 1007 1108 1141 1215
Size: 704 303 101 33 74
Fragments arranged by size:
704 303 101 74 33
BsmI GAATG_Cn'
Cuts at: 0 521 1215
Size: 521 694
Bsp1286I G_dGCh'C
Cuts at: 0 149 231 314 704 1007 1108 1141 1168
Size: 149 82 83 390 303 101 33 27
Cuts at: 1168 1184 1215
Size: 16 31
Fragments arranged by size:
390 303 149 101 83 82 33 31
27 16
BspHI T'CATG_A
Cuts at: 0 1100 1215
Size: 1100 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 226 1215
Size: 95 131 989
Fragments arranged by size:
989 131 95
BsrBI CCG'CTC
Cuts at: 0 657 717 1215
Size: 657 60 498
Fragments arranged by size:
657 498 60
BsrFI r'CCGG_y
Cuts at: 0 857 1215
Size: 857 358
BssSI C'ACGA_G
Cuts at: 0 678 1215
Size: 678 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 248 1215
Size: 33 215 967
Fragments arranged by size:
967 215 33
BstYI r'GATC_y
Cuts at: 0 200 383 1215
Size: 200 183 832
Fragments arranged by size:
832 200 183
BtgI C'CryG_G
Cuts at: 0 26 428 587 1215
Size: 26 402 159 628
Fragments arranged by size:
628 402 159 26
ClaI AT'CG_AT
Cuts at: 0 152 596 1215
Size: 152 444 619
Fragments arranged by size:
619 444 152
DraIII CAC_nnn'GTG
Cuts at: 0 101 1215
Size: 101 1114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 865 1215
Size: 865 350
EcoICRI GAG'CTC
Cuts at: 0 1005 1139 1215
Size: 1005 134 76
Fragments arranged by size:
1005 134 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 494 528 823 850 856 865 898
Size: 148 346 34 295 27 6 9 33
Cuts at: 898 955 1145 1215
Size: 57 190 70
Fragments arranged by size:
346 295 190 148 70 57 34 33
27 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1159 1215
Size: 1159 56
EcoO109I rG'GnC_Cy
Cuts at: 0 840 1189 1215
Size: 840 349 26
Fragments arranged by size:
840 349 26
HaeII r_GCGC'y
Cuts at: 0 536 668 1067 1155 1215
Size: 536 132 399 88 60
Fragments arranged by size:
536 399 132 88 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 1102 1134 1215
Size: 145 32 925 32 81
Fragments arranged by size:
925 145 81 32 32
KasI G'GCGC_C
Cuts at: 0 1063 1215
Size: 1063 152
MslI CAynn'nnrTG
Cuts at: 0 514 1215
Size: 514 701
MspA1I CmG'CkG
Cuts at: 0 142 270 737 834 870 876 951 1116
Size: 142 128 467 97 36 6 75 165
Cuts at: 1116 1123 1215
Size: 7 92
Fragments arranged by size:
467 165 142 128 97 92 75 36
7 6
NarI GG'CG_CC
Cuts at: 0 1064 1215
Size: 1064 151
NcoI C'CATG_G
Cuts at: 0 26 1215
Size: 26 1189
NspI r_CATG'y
Cuts at: 0 414 1215
Size: 414 801
PasI CC'CwG_GG
Cuts at: 0 990 1184 1215
Size: 990 194 31
Fragments arranged by size:
990 194 31
PflMI CCAn_nnn'nTGG
Cuts at: 0 1155 1215
Size: 1155 60
PfoI T'CCnGG_A
Cuts at: 0 569 932 1215
Size: 569 363 283
Fragments arranged by size:
569 363 283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 1215
Size: 754 32 429
Fragments arranged by size:
754 429 32
PpuMI rG'GwC_Cy
Cuts at: 0 840 1189 1215
Size: 840 349 26
Fragments arranged by size:
840 349 26
PstI C_TGCA'G
Cuts at: 0 304 875 944 1215
Size: 304 571 69 271
Fragments arranged by size:
571 304 271 69
PvuII CAG'CTG
Cuts at: 0 737 834 870 876 951 1123 1215
Size: 737 97 36 6 75 172 92
Fragments arranged by size:
737 172 97 92 75 36 6
SacI G_AGCT'C
Cuts at: 0 1007 1141 1215
Size: 1007 134 74
Fragments arranged by size:
1007 134 74
SanDI GG'GwC_CC
Cuts at: 0 1189 1215
Size: 1189 26
SfcI C'TryA_G
Cuts at: 0 300 871 940 1215
Size: 300 571 69 275
Fragments arranged by size:
571 300 275 69
SfoI GGC'GCC
Cuts at: 0 1065 1215
Size: 1065 150
SmaI CCC'GGG
Cuts at: 0 364 1215
Size: 364 851
SrfI GCCC'GGGC
Cuts at: 0 364 1215
Size: 364 851
StyI C'CwwG_G
Cuts at: 0 26 368 998 1215
Size: 26 342 630 217
Fragments arranged by size:
630 342 217 26
TatI w'GTAC_w
Cuts at: 0 134 1215
Size: 134 1081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 1215
Size: 30 1185
XmaI C'CCGG_G
Cuts at: 0 362 1215
Size: 362 853
Enzymes that do cut:
AccI AcuI AlfI AloI AlwNI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BclI BglI Bme1580I BmrI
BplI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstXI BstYI BtgI ClaI DraIII EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I
NarI NcoI NspI PasI PflMI PfoI PpiI PpuMI
PstI PvuII SacI SanDI SfcI SfoI SmaI SrfI
StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BfrBI BglII BlpI BmgBI
BmtI BpuEI BsaI BsaAI BsaBI BsiEI BsiWI BsmBI
BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI