Restriction map for
pFN21AE3966
(Linear) (Six-base) MAPSORT of: KIEE3966.seq Check: 6412 from: 1 to: 1041
KIEE3966.orf
With 182 enzymes: SgfI *
March 22, 2012 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 724 1041
Size: 488 236 317
Fragments arranged by size:
488 317 236
AflIII A'CryG_T
Cuts at: 0 380 1041
Size: 380 661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 613 1041
Size: 613 428
AlwNI CAG_nnn'CTG
Cuts at: 0 101 681 1041
Size: 101 580 360
Fragments arranged by size:
580 360 101
ApaLI G'TGCA_C
Cuts at: 0 284 1041
Size: 284 757
ApoI r'AATT_y
Cuts at: 0 962 1041
Size: 962 79
AvaI C'yCGr_G
Cuts at: 0 404 1041
Size: 404 637
BanII G_rGCy'C
Cuts at: 0 259 692 834 1041
Size: 259 433 142 207
Fragments arranged by size:
433 259 207 142
BbsI GAAGACnn'nnnn_
Cuts at: 0 517 629 1041
Size: 517 112 412
Fragments arranged by size:
517 412 112
BbvCI CC'TCA_GC
Cuts at: 0 445 1041
Size: 445 596
BclI T'GATC_A
Cuts at: 0 276 1041
Size: 276 765
BglI GCCn_nnn'nGGC
Cuts at: 0 210 1041
Size: 210 831
BglII A'GATC_T
Cuts at: 0 699 1041
Size: 699 342
Bme1580I G_kGCm'C
Cuts at: 0 288 1041
Size: 288 753
BmrI ACTGGGnnnn_n'
Cuts at: 0 588 669 900 1041
Size: 588 81 231 141
Fragments arranged by size:
588 231 141 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 711 1041
Size: 711 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 457 628 724 1041
Size: 409 48 171 96 317
Fragments arranged by size:
409 317 171 96 48
Bpu10I CC'TnA_GC
Cuts at: 0 76 238 445 1041
Size: 76 162 207 596
Fragments arranged by size:
596 207 162 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 362 1041
Size: 362 679
BsaHI Gr'CG_yC
Cuts at: 0 755 1041
Size: 755 286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 1041
Size: 235 30 776
Fragments arranged by size:
776 235 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 409 598 631 1041
Size: 46 363 189 33 410
Fragments arranged by size:
410 363 189 46 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1041
Size: 685 356
BsiHKAI G_wGCw'C
Cuts at: 0 288 368 692 834 1041
Size: 288 80 324 142 207
Fragments arranged by size:
324 288 207 142 80
BsmI GAATG_Cn'
Cuts at: 0 854 1041
Size: 854 187
Bsp1286I G_dGCh'C
Cuts at: 0 259 288 368 692 834 1041
Size: 259 29 80 324 142 207
Fragments arranged by size:
324 259 207 142 80 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 1041
Size: 49 992
BsrBI CCG'CTC
Cuts at: 0 354 1041
Size: 354 687
BsrDI GCAATG_nn'
Cuts at: 0 597 1041
Size: 597 444
BstAPI GCAn_nnn'nTGC
Cuts at: 0 714 1041
Size: 714 327
BstEII G'GTnAC_C
Cuts at: 0 36 373 1041
Size: 36 337 668
Fragments arranged by size:
668 337 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 228 543 1041
Size: 54 174 315 498
Fragments arranged by size:
498 315 174 54
BstYI r'GATC_y
Cuts at: 0 699 1041
Size: 699 342
BtgI C'CryG_G
Cuts at: 0 291 369 1041
Size: 291 78 672
Fragments arranged by size:
672 291 78
BtsI GCAGTG_nn'
Cuts at: 0 116 1041
Size: 116 925
DrdI GACnn_nn'nnGTC
Cuts at: 0 331 1041
Size: 331 710
EaeI y'GGCC_r
Cuts at: 0 90 1041
Size: 90 951
EarI CTCTTCn'nnn_
Cuts at: 0 316 464 488 1041
Size: 316 148 24 553
Fragments arranged by size:
553 316 148 24
EcoICRI GAG'CTC
Cuts at: 0 690 832 1041
Size: 690 142 209
Fragments arranged by size:
690 209 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 457 488 628 724 1041
Size: 409 48 31 140 96 317
Fragments arranged by size:
409 317 140 96 48 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 610 1041
Size: 391 219 431
Fragments arranged by size:
431 391 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 677 709 1041
Size: 677 32 332
Fragments arranged by size:
677 332 32
HindIII A'AGCT_T
Cuts at: 0 500 1041
Size: 500 541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 906 1041
Size: 455 451 135
Fragments arranged by size:
455 451 135
NcoI C'CATG_G
Cuts at: 0 291 1041
Size: 291 750
NspI r_CATG'y
Cuts at: 0 113 217 1041
Size: 113 104 824
Fragments arranged by size:
824 113 104
PfoI T'CCnGG_A
Cuts at: 0 505 572 1041
Size: 505 67 469
Fragments arranged by size:
505 469 67
PshAI GACnn'nnGTC
Cuts at: 0 877 1041
Size: 877 164
PstI C_TGCA'G
Cuts at: 0 719 1041
Size: 719 322
SacI G_AGCT'C
Cuts at: 0 692 834 1041
Size: 692 142 207
Fragments arranged by size:
692 207 142
SfcI C'TryA_G
Cuts at: 0 715 1041
Size: 715 326
SmaI CCC'GGG
Cuts at: 0 406 1041
Size: 406 635
SphI G_CATG'C
Cuts at: 0 113 1041
Size: 113 928
StyI C'CwwG_G
Cuts at: 0 291 1041
Size: 291 750
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 1041
Size: 118 32 891
Fragments arranged by size:
891 118 32
Tth111I GACn'n_nGTC
Cuts at: 0 675 1041
Size: 675 366
XmaI C'CCGG_G
Cuts at: 0 404 1041
Size: 404 637
XmnI GAAnn'nnTTC
Cuts at: 0 626 800 1041
Size: 626 174 241
Fragments arranged by size:
626 241 174
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaLI ApoI AvaI BanII
BbsI BbvCI BclI BglI BglII Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BstAPI BstEII BstXI
BstYI BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI Hin4I HindIII MmeI NcoI NspI PfoI PshAI
PstI SacI SfcI SmaI SphI StyI TstI Tth111I
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BfrBI BlpI
BmgBI BmtI BpuEI BsaAI BsaBI BsaWI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TatI XbaI XcmI
XhoI ZraI