(Linear) (Six-base) MAPSORT of: KIEE3964.seq  Check: 4803  from: 1  to: 915



KIEE3964.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:00  ..

AclI AA'CG_TT

Cuts at:      0     710     915 
   Size:        710     205
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     608     915 
   Size:        608     307
 
AlwNI CAG_nnn'CTG

Cuts at:      0     316     360     915 
   Size:        316      44     555

  Fragments arranged by size: 

                 555     316      44
 
ApoI r'AATT_y

Cuts at:      0     445     890     915 
   Size:        445     445      25

  Fragments arranged by size: 

                 445     445      25
 
BclI T'GATC_A

Cuts at:      0     813     915 
   Size:        813     102
 
BglI GCCn_nnn'nGGC

Cuts at:      0     670     915 
   Size:        670     245
 
BglII A'GATC_T

Cuts at:      0     644     915 
   Size:        644     271
 
BlpI GC'TnA_GC

Cuts at:      0     682     915 
   Size:        682     233
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     348     915 
   Size:        348     567
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     915 
   Size:        199     716
 
BseYI C'CCAG_C

Cuts at:      0     192     605     915 
   Size:        192     413     310

  Fragments arranged by size: 

                 413     310     192
 
BsmI GAATG_Cn'

Cuts at:      0     715     915 
   Size:        715     200
 
BsrBI CCG'CTC

Cuts at:      0     686     915 
   Size:        686     229
 
BsrDI GCAATG_nn'

Cuts at:      0     230     915 
   Size:        230     685
 
BsrGI T'GTAC_A

Cuts at:      0     654     915 
   Size:        654     261
 
BssSI C'ACGA_G

Cuts at:      0     435     915 
   Size:        435     480
 
BstYI r'GATC_y

Cuts at:      0     644     915 
   Size:        644     271
 
BtsI GCAGTG_nn'

Cuts at:      0     617     915 
   Size:        617     298
 
EaeI y'GGCC_r

Cuts at:      0     362     687     915 
   Size:        362     325     228

  Fragments arranged by size: 

                 362     325     228
 
EarI CTCTTCn'nnn_

Cuts at:      0      28     915 
   Size:         28     887
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     608     915 
   Size:        199     409     307

  Fragments arranged by size: 

                 409     307     199
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     130     915 
   Size:        130     785
 
EcoO109I rG'GnC_Cy

Cuts at:      0     313     915 
   Size:        313     602
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     762     855     915 
   Size:        762      93      60

  Fragments arranged by size: 

                 762      93      60
 
MscI TGG'CCA

Cuts at:      0     364     915 
   Size:        364     551
 
MslI CAynn'nnrTG

Cuts at:      0      47     899     915 
   Size:         47     852      16

  Fragments arranged by size: 

                 852      47      16
 
MspA1I CmG'CkG

Cuts at:      0     192     915 
   Size:        192     723
 
NspI r_CATG'y

Cuts at:      0     664     915 
   Size:        664     251
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     360     915 
   Size:        360     555
 
PpuMI rG'GwC_Cy

Cuts at:      0     313     915 
   Size:        313     602
 
PshAI GACnn'nnGTC

Cuts at:      0     103     915 
   Size:        103     812
 
PvuII CAG'CTG

Cuts at:      0     192     915 
   Size:        192     723
 
SexAI A'CCwGG_T

Cuts at:      0     125     915 
   Size:        125     790
 
SfcI C'TryA_G

Cuts at:      0     166     915 
   Size:        166     749
 
SspI AAT'ATT

Cuts at:      0     424     519     915 
   Size:        424      95     396

  Fragments arranged by size: 

                 424     396      95
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     690     915 
   Size:        690     225
 
TatI w'GTAC_w

Cuts at:      0     654     915 
   Size:        654     261
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     132     154     164     186     727     759     915 
   Size:        132      22      10      22     541      32     156

  Fragments arranged by size: 

                 541     156     132      32      22      22      10
 
XmnI GAAnn'nnTTC

Cuts at:      0     855     915 
   Size:        855      60
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     BclI     BglI    BglII     BlpI
     BmrI     BpmI    BseYI     BsmI    BsrBI    BsrDI    BsrGI    BssSI
    BstYI     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I     MmeI
     MscI     MslI   MspA1I     NspI    PflMI    PpuMI    PshAI    PvuII
    SexAI     SfcI     SspI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrFI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI