Restriction map for
pFN21AE3963
(Linear) (Six-base) MAPSORT of: KIEE3963.seq Check: 650 from: 1 to: 1404
KIEE3963.orf
With 182 enzymes: SgfI *
March 22, 2012 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 52 1404
Size: 52 1352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1404
Size: 929 475
AfeI AGC'GCT
Cuts at: 0 346 970 1006 1404
Size: 346 624 36 398
Fragments arranged by size:
624 398 346 36
AflIII A'CryG_T
Cuts at: 0 152 1404
Size: 152 1252
AlwNI CAG_nnn'CTG
Cuts at: 0 585 588 597 951 1404
Size: 585 3 9 354 453
Fragments arranged by size:
585 453 354 9 3
ApaI G_GGCC'C
Cuts at: 0 148 1065 1404
Size: 148 917 339
Fragments arranged by size:
917 339 148
AvaI C'yCGr_G
Cuts at: 0 139 160 694 825 1288 1302 1404
Size: 139 21 534 131 463 14 102
Fragments arranged by size:
534 463 139 131 102 21 14
AvrII C'CTAG_G
Cuts at: 0 651 1404
Size: 651 753
BamHI G'GATC_C
Cuts at: 0 72 1133 1404
Size: 72 1061 271
Fragments arranged by size:
1061 271 72
BanI G'GyrC_C
Cuts at: 0 236 1404
Size: 236 1168
BanII G_rGCy'C
Cuts at: 0 148 683 695 1065 1404
Size: 148 535 12 370 339
Fragments arranged by size:
535 370 339 148 12
BbeI G_GCGC'C
Cuts at: 0 240 1404
Size: 240 1164
BbsI GAAGACnn'nnnn_
Cuts at: 0 404 544 917 1134 1404
Size: 404 140 373 217 270
Fragments arranged by size:
404 373 270 217 140
BbvCI CC'TCA_GC
Cuts at: 0 805 1404
Size: 805 599
BglI GCCn_nnn'nGGC
Cuts at: 0 229 1404
Size: 229 1175
BlpI GC'TnA_GC
Cuts at: 0 677 1404
Size: 677 727
Bme1580I G_kGCm'C
Cuts at: 0 148 160 277 1065 1404
Size: 148 12 117 788 339
Fragments arranged by size:
788 339 148 117 12
BmgBI CAC'GTC
Cuts at: 0 864 1404
Size: 864 540
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 1038 1404
Size: 369 669 366
Fragments arranged by size:
669 369 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 436 448 619 634 1404
Size: 13 423 12 171 15 770
Fragments arranged by size:
770 423 171 15 13 12
Bpu10I CC'TnA_GC
Cuts at: 0 805 893 1404
Size: 805 88 511
Fragments arranged by size:
805 511 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1404
Size: 388 1016
BsaI GGTCTCn'nnnn_
Cuts at: 0 478 1404
Size: 478 926
BsaBI GATnn'nnATC
Cuts at: 0 1357 1404
Size: 1357 47
BsaHI Gr'CG_yC
Cuts at: 0 237 746 1404
Size: 237 509 658
Fragments arranged by size:
658 509 237
BsaWI w'CCGG_w
Cuts at: 0 75 437 1404
Size: 75 362 967
Fragments arranged by size:
967 362 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1380 1404
Size: 1380 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 586 589 616 619 622 1404
Size: 49 537 3 27 3 3 782
Fragments arranged by size:
782 537 49 27 3 3 3
BseYI C'CCAG_C
Cuts at: 0 128 534 993 1058 1404
Size: 128 406 459 65 346
Fragments arranged by size:
459 406 346 128 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 837 946 1095 1404
Size: 226 611 109 149 309
Fragments arranged by size:
611 309 226 149 109
BsiEI CG_ry'CG
Cuts at: 0 379 395 1369 1404
Size: 379 16 974 35
Fragments arranged by size:
974 379 35 16
BsiHKAI G_wGCw'C
Cuts at: 0 359 683 695 1404
Size: 359 324 12 709
Fragments arranged by size:
709 359 324 12
BsiWI C'GTAC_G
Cuts at: 0 149 1404
Size: 149 1255
Bsp1286I G_dGCh'C
Cuts at: 0 148 160 277 359 683 695 1065 1404
Size: 148 12 117 82 324 12 370 339
Fragments arranged by size:
370 339 324 148 117 82 12 12
BspEI T'CCGG_A
Cuts at: 0 437 1404
Size: 437 967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 200 496 1404
Size: 52 148 296 908
Fragments arranged by size:
908 296 148 52
BsrFI r'CCGG_y
Cuts at: 0 255 1364 1404
Size: 255 1109 40
Fragments arranged by size:
1109 255 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 372 531 585 588 951 1404
Size: 372 159 54 3 363 453
Fragments arranged by size:
453 372 363 159 54 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1209 1404
Size: 1209 195
BstYI r'GATC_y
Cuts at: 0 72 1133 1214 1292 1404
Size: 72 1061 81 78 112
Fragments arranged by size:
1061 112 81 78 72
Bsu36I CC'TnA_GG
Cuts at: 0 1014 1404
Size: 1014 390
BtgI C'CryG_G
Cuts at: 0 1115 1404
Size: 1115 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 669 1404
Size: 669 735
BtsI GCAGTG_nn'
Cuts at: 0 841 1270 1404
Size: 841 429 134
Fragments arranged by size:
841 429 134
DraIII CAC_nnn'GTG
Cuts at: 0 372 1404
Size: 372 1032
EaeI y'GGCC_r
Cuts at: 0 2 93 221 296 376 392 1366 1404
Size: 2 91 128 75 80 16 974 38
Fragments arranged by size:
974 128 91 80 75 38 16 2
EagI C'GGCC_G
Cuts at: 0 376 392 1366 1404
Size: 376 16 974 38
Fragments arranged by size:
974 376 38 16
EarI CTCTTCn'nnn_
Cuts at: 0 1327 1404
Size: 1327 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 493 933 1404
Size: 493 440 471
Fragments arranged by size:
493 471 440
EcoICRI GAG'CTC
Cuts at: 0 681 693 1404
Size: 681 12 711
Fragments arranged by size:
711 681 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 436 448 619 634 929 1404
Size: 13 423 12 171 15 295 475
Fragments arranged by size:
475 423 295 171 15 13 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 943 1404
Size: 31 912 461
Fragments arranged by size:
912 461 31
EcoO109I rG'GnC_Cy
Cuts at: 0 144 822 936 948 1062 1194 1404
Size: 144 678 114 12 114 132 210
Fragments arranged by size:
678 210 144 132 114 114 12
FspI TGC'GCA
Cuts at: 0 754 1404
Size: 754 650
HaeII r_GCGC'y
Cuts at: 0 126 240 348 668 972 1008 1404
Size: 126 114 108 320 304 36 396
Fragments arranged by size:
396 320 304 126 114 108 36
KasI G'GCGC_C
Cuts at: 0 236 1404
Size: 236 1168
MluI A'CGCG_T
Cuts at: 0 152 1404
Size: 152 1252
MspA1I CmG'CkG
Cuts at: 0 381 522 585 704 711 930 1058 1117
Size: 381 141 63 119 7 219 128 59
Cuts at: 1117 1404
Size: 287
Fragments arranged by size:
381 287 219 141 128 119 63 59
7
NaeI GCC'GGC
Cuts at: 0 1366 1404
Size: 1366 38
NarI GG'CG_CC
Cuts at: 0 237 1404
Size: 237 1167
NgoMIV G'CCGG_C
Cuts at: 0 1364 1404
Size: 1364 40
NotI GC'GGCC_GC
Cuts at: 0 376 1404
Size: 376 1028
PasI CC'CwG_GG
Cuts at: 0 1065 1404
Size: 1065 339
PfoI T'CCnGG_A
Cuts at: 0 29 761 1190 1404
Size: 29 732 429 214
Fragments arranged by size:
732 429 214 29
PpuMI rG'GwC_Cy
Cuts at: 0 822 936 1194 1404
Size: 822 114 258 210
Fragments arranged by size:
822 258 210 114
PshAI GACnn'nnGTC
Cuts at: 0 490 1404
Size: 490 914
PspOMI G'GGCC_C
Cuts at: 0 144 1061 1404
Size: 144 917 343
Fragments arranged by size:
917 343 144
PspXI vC'TCGA_Gb
Cuts at: 0 694 1404
Size: 694 710
PstI C_TGCA'G
Cuts at: 0 386 593 734 779 1404
Size: 386 207 141 45 625
Fragments arranged by size:
625 386 207 141 45
PvuII CAG'CTG
Cuts at: 0 585 711 1058 1404
Size: 585 126 347 346
Fragments arranged by size:
585 347 346 126
SacI G_AGCT'C
Cuts at: 0 683 695 1404
Size: 683 12 709
Fragments arranged by size:
709 683 12
SacII CC_GC'GG
Cuts at: 0 1118 1404
Size: 1118 286
SanDI GG'GwC_CC
Cuts at: 0 822 1194 1404
Size: 822 372 210
Fragments arranged by size:
822 372 210
SfcI C'TryA_G
Cuts at: 0 382 589 730 775 1251 1404
Size: 382 207 141 45 476 153
Fragments arranged by size:
476 382 207 153 141 45
SfoI GGC'GCC
Cuts at: 0 238 1404
Size: 238 1166
SmaI CCC'GGG
Cuts at: 0 827 1404
Size: 827 577
SmlI C'TyrA_G
Cuts at: 0 403 694 1404
Size: 403 291 710
Fragments arranged by size:
710 403 291
StyI C'CwwG_G
Cuts at: 0 651 869 1178 1404
Size: 651 218 309 226
Fragments arranged by size:
651 309 226 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 526 1215 1404
Size: 100 426 689 189
Fragments arranged by size:
689 426 189 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1380 1404
Size: 1380 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 1404
Size: 1062 342
XhoI C'TCGA_G
Cuts at: 0 694 1404
Size: 694 710
XmaI C'CCGG_G
Cuts at: 0 825 1404
Size: 825 579
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspMI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII KasI
MluI MspA1I NaeI NarI NgoMIV NotI PasI PfoI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SanDI SfcI SfoI SmaI SmlI StyI TaqII TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BcgI BciVI BclI BfrBI BglII BmtI BplI BsaAI
BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MmeI MscI MslI NcoI NdeI NheI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PsiI PsrI PvuI RsrII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XmnI
ZraI