Restriction map for
pFN21AE3960
(Linear) (Six-base) MAPSORT of: KIEE3960.seq Check: 2032 from: 1 to: 1686
KIEE3960.orf
With 182 enzymes: SgfI *
March 22, 2012 16:59 ..
AccI GT'mk_AC
Cuts at: 0 1665 1686
Size: 1665 21
Acc65I G'GTAC_C
Cuts at: 0 1544 1686
Size: 1544 142
AclI AA'CG_TT
Cuts at: 0 1259 1686
Size: 1259 427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1686
Size: 907 779
AflIII A'CryG_T
Cuts at: 0 151 1686
Size: 151 1535
AleI CACnn'nnGTG
Cuts at: 0 517 668 1686
Size: 517 151 1018
Fragments arranged by size:
1018 517 151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1161 1686
Size: 1161 525
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1244 1276 1686
Size: 1244 32 410
Fragments arranged by size:
1244 410 32
AlwNI CAG_nnn'CTG
Cuts at: 0 396 960 1686
Size: 396 564 726
Fragments arranged by size:
726 564 396
ApaI G_GGCC'C
Cuts at: 0 912 1686
Size: 912 774
ApoI r'AATT_y
Cuts at: 0 921 1160 1609 1686
Size: 921 239 449 77
Fragments arranged by size:
921 449 239 77
AvaI C'yCGr_G
Cuts at: 0 1181 1686
Size: 1181 505
AvrII C'CTAG_G
Cuts at: 0 1548 1686
Size: 1548 138
BanI G'GyrC_C
Cuts at: 0 1528 1544 1686
Size: 1528 16 142
Fragments arranged by size:
1528 142 16
BanII G_rGCy'C
Cuts at: 0 173 722 912 1182 1417 1686
Size: 173 549 190 270 235 269
Fragments arranged by size:
549 270 269 235 190 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 905 1686
Size: 905 781
Bme1580I G_kGCm'C
Cuts at: 0 795 912 1686
Size: 795 117 774
Fragments arranged by size:
795 774 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 1294 1686
Size: 1294 392
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 192 1686
Size: 192 1494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 187 388 775 781 998 1118 1473
Size: 178 9 201 387 6 217 120 355
Cuts at: 1473 1686
Size: 213
Fragments arranged by size:
387 355 217 213 201 178 120 9
6
Bpu10I CC'TnA_GC
Cuts at: 0 296 1060 1686
Size: 296 764 626
Fragments arranged by size:
764 626 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1686
Size: 226 1460
BsaAI yAC'GTr
Cuts at: 0 152 379 744 1686
Size: 152 227 365 942
Fragments arranged by size:
942 365 227 152
BsaBI GATnn'nnATC
Cuts at: 0 271 1030 1686
Size: 271 759 656
Fragments arranged by size:
759 656 271
BsaHI Gr'CG_yC
Cuts at: 0 1370 1686
Size: 1370 316
BsaWI w'CCGG_w
Cuts at: 0 830 1686
Size: 830 856
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1244 1274 1686
Size: 1244 30 412
Fragments arranged by size:
1244 412 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 712 880 1265 1452 1674 1686
Size: 712 168 385 187 222 12
Fragments arranged by size:
712 385 222 187 168 12
BseYI C'CCAG_C
Cuts at: 0 24 944 1686
Size: 24 920 742
Fragments arranged by size:
920 742 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1429 1461 1686
Size: 41 1388 32 225
Fragments arranged by size:
1388 225 41 32
BsiHKAI G_wGCw'C
Cuts at: 0 722 998 1686
Size: 722 276 688
Fragments arranged by size:
722 688 276
BsmI GAATG_Cn'
Cuts at: 0 1633 1686
Size: 1633 53
Bsp1286I G_dGCh'C
Cuts at: 0 173 722 795 912 998 1182 1417 1686
Size: 173 549 73 117 86 184 235 269
Fragments arranged by size:
549 269 235 184 173 117 86 73
BspEI T'CCGG_A
Cuts at: 0 830 1686
Size: 830 856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 454 1686
Size: 314 140 1232
Fragments arranged by size:
1232 314 140
BsrBI CCG'CTC
Cuts at: 0 1510 1686
Size: 1510 176
BsrDI GCAATG_nn'
Cuts at: 0 690 1686
Size: 690 996
BsrFI r'CCGG_y
Cuts at: 0 620 1686
Size: 620 1066
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1484 1686
Size: 1484 202
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 700 1044 1150 1686
Size: 156 544 344 106 536
Fragments arranged by size:
544 536 344 156 106
BstYI r'GATC_y
Cuts at: 0 773 1025 1686
Size: 773 252 661
Fragments arranged by size:
773 661 252
Bsu36I CC'TnA_GG
Cuts at: 0 1127 1686
Size: 1127 559
BtgI C'CryG_G
Cuts at: 0 311 515 1149 1560 1686
Size: 311 204 634 411 126
Fragments arranged by size:
634 411 311 204 126
BtsI GCAGTG_nn'
Cuts at: 0 81 821 1470 1686
Size: 81 740 649 216
Fragments arranged by size:
740 649 216 81
DraIII CAC_nnn'GTG
Cuts at: 0 152 1686
Size: 152 1534
DrdI GACnn_nn'nnGTC
Cuts at: 0 1300 1686
Size: 1300 386
EaeI y'GGCC_r
Cuts at: 0 347 1557 1686
Size: 347 1210 129
Fragments arranged by size:
1210 347 129
EarI CTCTTCn'nnn_
Cuts at: 0 427 1686
Size: 427 1259
EcoICRI GAG'CTC
Cuts at: 0 720 1686
Size: 720 966
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 187 388 775 781 907 998 1118
Size: 178 9 201 387 6 126 91 120
Cuts at: 1118 1473 1686
Size: 355 213
Fragments arranged by size:
387 355 213 201 178 126 120 91
9 6
EcoO109I rG'GnC_Cy
Cuts at: 0 393 750 1686
Size: 393 357 936
Fragments arranged by size:
936 393 357
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1134 1686
Size: 1134 552
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 1665 1686
Size: 199 32 1434 21
Fragments arranged by size:
1434 199 32 21
KpnI G_GTAC'C
Cuts at: 0 1548 1686
Size: 1548 138
MscI TGG'CCA
Cuts at: 0 1559 1686
Size: 1559 127
MslI CAynn'nnrTG
Cuts at: 0 517 668 698 1686
Size: 517 151 30 988
Fragments arranged by size:
988 517 151 30
MspA1I CmG'CkG
Cuts at: 0 165 823 894 933 948 1010 1493 1686
Size: 165 658 71 39 15 62 483 193
Fragments arranged by size:
658 483 193 165 71 62 39 15
NcoI C'CATG_G
Cuts at: 0 311 515 1560 1686
Size: 311 204 1045 126
Fragments arranged by size:
1045 311 204 126
NspI r_CATG'y
Cuts at: 0 972 1408 1686
Size: 972 436 278
Fragments arranged by size:
972 436 278
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 516 1686
Size: 396 120 1170
Fragments arranged by size:
1170 396 120
PfoI T'CCnGG_A
Cuts at: 0 233 1095 1316 1686
Size: 233 862 221 370
Fragments arranged by size:
862 370 233 221
PmlI CAC'GTG
Cuts at: 0 152 744 1686
Size: 152 592 942
Fragments arranged by size:
942 592 152
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1244 1276 1686
Size: 1244 32 410
Fragments arranged by size:
1244 410 32
PspOMI G'GGCC_C
Cuts at: 0 908 1686
Size: 908 778
PstI C_TGCA'G
Cuts at: 0 260 588 932 938 1686
Size: 260 328 344 6 748
Fragments arranged by size:
748 344 328 260 6
PvuII CAG'CTG
Cuts at: 0 165 823 894 933 948 1686
Size: 165 658 71 39 15 738
Fragments arranged by size:
738 658 165 71 39 15
SacI G_AGCT'C
Cuts at: 0 722 1686
Size: 722 964
SfcI C'TryA_G
Cuts at: 0 256 543 584 928 934 1686
Size: 256 287 41 344 6 752
Fragments arranged by size:
752 344 287 256 41 6
SmaI CCC'GGG
Cuts at: 0 1183 1686
Size: 1183 503
SmlI C'TyrA_G
Cuts at: 0 241 1686
Size: 241 1445
SpeI A'CTAG_T
Cuts at: 0 46 1686
Size: 46 1640
SspI AAT'ATT
Cuts at: 0 230 1686
Size: 230 1456
StyI C'CwwG_G
Cuts at: 0 311 515 1358 1548 1560 1686
Size: 311 204 843 190 12 126
Fragments arranged by size:
843 311 204 190 126 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 304 1592 1686
Size: 304 1288 94
Fragments arranged by size:
1288 304 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 697 729 1686
Size: 697 32 957
Fragments arranged by size:
957 697 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 1044 1686
Size: 342 702 642
Fragments arranged by size:
702 642 342
XmaI C'CCGG_G
Cuts at: 0 1181 1686
Size: 1181 505
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I FalI Hin4I KpnI MscI MslI
MspA1I NcoI NspI PflMI PfoI PmlI PpiI PspOMI
PstI PvuII SacI SfcI SmaI SmlI SpeI SspI
StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaLI AscI
AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaI
BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SphI SrfI StuI
SwaI TatI Tth111I XbaI XhoI XmnI ZraI