Restriction map for
pFN21AE3958
(Linear) (Six-base) MAPSORT of: KIEE3958.seq Check: 1905 from: 1 to: 1338
KIEE3958.orf
With 182 enzymes: SgfI *
March 22, 2012 16:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 49 1338
Size: 49 1289
AccI GT'mk_AC
Cuts at: 0 68 1338
Size: 68 1270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1150 1338
Size: 229 921 188
Fragments arranged by size:
921 229 188
AlwNI CAG_nnn'CTG
Cuts at: 0 101 245 1338
Size: 101 144 1093
Fragments arranged by size:
1093 144 101
ApoI r'AATT_y
Cuts at: 0 213 863 948 1338
Size: 213 650 85 390
Fragments arranged by size:
650 390 213 85
BanII G_rGCy'C
Cuts at: 0 262 369 809 1050 1338
Size: 262 107 440 241 288
Fragments arranged by size:
440 288 262 241 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 984 1338
Size: 984 354
BbvCI CC'TCA_GC
Cuts at: 0 398 1338
Size: 398 940
BclI T'GATC_A
Cuts at: 0 533 1338
Size: 533 805
BglII A'GATC_T
Cuts at: 0 958 1338
Size: 958 380
Bme1580I G_kGCm'C
Cuts at: 0 95 528 1338
Size: 95 433 810
Fragments arranged by size:
810 433 95
BmgBI CAC'GTC
Cuts at: 0 937 1338
Size: 937 401
BmrI ACTGGGnnnn_n'
Cuts at: 0 1023 1053 1162 1338
Size: 1023 30 109 176
Fragments arranged by size:
1023 176 109 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 619 718 1338
Size: 522 97 99 620
Fragments arranged by size:
620 522 99 97
Bpu10I CC'TnA_GC
Cuts at: 0 256 398 1338
Size: 256 142 940
Fragments arranged by size:
940 256 142
BsaWI w'CCGG_w
Cuts at: 0 561 1338
Size: 561 777
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 737 767 974 1004 1338
Size: 737 30 207 30 334
Fragments arranged by size:
737 334 207 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 493 1338
Size: 319 174 845
Fragments arranged by size:
845 319 174
BseYI C'CCAG_C
Cuts at: 0 788 1284 1338
Size: 788 496 54
Fragments arranged by size:
788 496 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1338
Size: 763 575
BsiHKAI G_wGCw'C
Cuts at: 0 262 346 362 809 1338
Size: 262 84 16 447 529
Fragments arranged by size:
529 447 262 84 16
Bsp1286I G_dGCh'C
Cuts at: 0 95 262 346 362 369 528 809 1050
Size: 95 167 84 16 7 159 281 241
Cuts at: 1050 1338
Size: 288
Fragments arranged by size:
288 281 241 167 159 95 84 16
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 1338
Size: 49 1289
BsrDI GCAATG_nn'
Cuts at: 0 224 516 1338
Size: 224 292 822
Fragments arranged by size:
822 292 224
BsrGI T'GTAC_A
Cuts at: 0 836 1338
Size: 836 502
BstYI r'GATC_y
Cuts at: 0 958 1338
Size: 958 380
BtsI GCAGTG_nn'
Cuts at: 0 809 1338
Size: 809 529
EaeI y'GGCC_r
Cuts at: 0 1329 1338
Size: 1329 9
EarI CTCTTCn'nnn_
Cuts at: 0 383 1338
Size: 383 955
EcoICRI GAG'CTC
Cuts at: 0 260 807 1338
Size: 260 547 531
Fragments arranged by size:
547 531 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 522 619 718 1150 1338
Size: 229 293 97 99 432 188
Fragments arranged by size:
432 293 229 188 99 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1074 1338
Size: 1074 264
EcoO109I rG'GnC_Cy
Cuts at: 0 107 281 817 1338
Size: 107 174 536 521
Fragments arranged by size:
536 521 174 107
EcoRI G'AATT_C
Cuts at: 0 948 1338
Size: 948 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 974 1006 1338
Size: 974 32 332
Fragments arranged by size:
974 332 32
HincII GTy'rAC
Cuts at: 0 1098 1338
Size: 1098 240
HpaI GTT'AAC
Cuts at: 0 1098 1338
Size: 1098 240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 904 1338
Size: 371 533 434
Fragments arranged by size:
533 434 371
MscI TGG'CCA
Cuts at: 0 1331 1338
Size: 1331 7
MspA1I CmG'CkG
Cuts at: 0 397 1288 1338
Size: 397 891 50
Fragments arranged by size:
891 397 50
NdeI CA'TA_TG
Cuts at: 0 1273 1338
Size: 1273 65
NspI r_CATG'y
Cuts at: 0 22 164 786 1338
Size: 22 142 622 552
Fragments arranged by size:
622 552 142 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 245 741 1338
Size: 245 496 597
Fragments arranged by size:
597 496 245
PfoI T'CCnGG_A
Cuts at: 0 734 1069 1338
Size: 734 335 269
Fragments arranged by size:
734 335 269
PpuMI rG'GwC_Cy
Cuts at: 0 281 817 1338
Size: 281 536 521
Fragments arranged by size:
536 521 281
PshAI GACnn'nnGTC
Cuts at: 0 323 1338
Size: 323 1015
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 595 627 1338
Size: 595 32 711
Fragments arranged by size:
711 595 32
PstI C_TGCA'G
Cuts at: 0 816 1338
Size: 816 522
PvuII CAG'CTG
Cuts at: 0 397 1288 1338
Size: 397 891 50
Fragments arranged by size:
891 397 50
SacI G_AGCT'C
Cuts at: 0 262 809 1338
Size: 262 547 529
Fragments arranged by size:
547 529 262
SanDI GG'GwC_CC
Cuts at: 0 281 1338
Size: 281 1057
SexAI A'CCwGG_T
Cuts at: 0 62 1338
Size: 62 1276
SfcI C'TryA_G
Cuts at: 0 102 812 1338
Size: 102 710 526
Fragments arranged by size:
710 526 102
SphI G_CATG'C
Cuts at: 0 164 1338
Size: 164 1174
SspI AAT'ATT
Cuts at: 0 201 1338
Size: 201 1137
StuI AGG'CCT
Cuts at: 0 1314 1338
Size: 1314 24
TatI w'GTAC_w
Cuts at: 0 836 1338
Size: 836 502
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8 40 737 769 1338
Size: 8 32 697 32 569
Fragments arranged by size:
697 569 32 32 8
Tth111I GACn'n_nGTC
Cuts at: 0 73 1338
Size: 73 1265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 828 1338
Size: 828 510
XmnI GAAnn'nnTTC
Cuts at: 0 304 1338
Size: 304 1034
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI BanII BbsI BbvCI
BclI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrGI BstYI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII HpaI MmeI MscI MspA1I
NdeI NspI PflMI PfoI PpuMI PshAI PsrI PstI
PvuII SacI SanDI SexAI SfcI SphI SspI StuI
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BfrBI BglI BlpI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StyI SwaI TaqII XbaI XhoI XmaI ZraI