Restriction map for
pFN21AE3957
(Linear) (Six-base) MAPSORT of: KIEE3957.seq Check: 6068 from: 1 to: 1023
KIEE3957.orf
With 182 enzymes: SgfI *
March 22, 2012 16:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 67 235 909 1023
Size: 40 27 168 674 114
Fragments arranged by size:
674 168 114 40 27
AflIII A'CryG_T
Cuts at: 0 126 1023
Size: 126 897
AlwNI CAG_nnn'CTG
Cuts at: 0 63 162 557 1023
Size: 63 99 395 466
Fragments arranged by size:
466 395 99 63
BanII G_rGCy'C
Cuts at: 0 197 631 1023
Size: 197 434 392
Fragments arranged by size:
434 392 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 688 1023
Size: 688 335
BmrI ACTGGGnnnn_n'
Cuts at: 0 634 669 1023
Size: 634 35 354
Fragments arranged by size:
634 354 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1023
Size: 55 968
Bpu10I CC'TnA_GC
Cuts at: 0 749 1023
Size: 749 274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 615 1023
Size: 594 21 408
Fragments arranged by size:
594 408 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 1023
Size: 217 30 776
Fragments arranged by size:
776 217 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 22 238 1023
Size: 19 3 216 785
Fragments arranged by size:
785 216 19 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1023
Size: 148 875
BsiHKAI G_wGCw'C
Cuts at: 0 197 536 1023
Size: 197 339 487
Fragments arranged by size:
487 339 197
BsmI GAATG_Cn'
Cuts at: 0 290 1023
Size: 290 733
Bsp1286I G_dGCh'C
Cuts at: 0 197 536 631 1023
Size: 197 339 95 392
Fragments arranged by size:
392 339 197 95
BsrDI GCAATG_nn'
Cuts at: 0 265 1000 1023
Size: 265 735 23
Fragments arranged by size:
735 265 23
Bsu36I CC'TnA_GG
Cuts at: 0 64 1023
Size: 64 959
BtgI C'CryG_G
Cuts at: 0 134 1023
Size: 134 889
EarI CTCTTCn'nnn_
Cuts at: 0 197 1023
Size: 197 826
EcoICRI GAG'CTC
Cuts at: 0 195 1023
Size: 195 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 55 67 235 909 1023
Size: 40 15 12 168 674 114
Fragments arranged by size:
674 168 114 40 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 752 844 1023
Size: 752 92 179
Fragments arranged by size:
752 179 92
EcoO109I rG'GnC_Cy
Cuts at: 0 433 1023
Size: 433 590
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 701 733 1023
Size: 701 32 290
Fragments arranged by size:
701 290 32
HincII GTy'rAC
Cuts at: 0 31 1023
Size: 31 992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 678 1023
Size: 45 633 345
Fragments arranged by size:
633 345 45
MslI CAynn'nnrTG
Cuts at: 0 937 1023
Size: 937 86
MspA1I CmG'CkG
Cuts at: 0 734 1023
Size: 734 289
NcoI C'CATG_G
Cuts at: 0 134 1023
Size: 134 889
NspI r_CATG'y
Cuts at: 0 75 130 1023
Size: 75 55 893
Fragments arranged by size:
893 75 55
PciI A'CATG_T
Cuts at: 0 126 1023
Size: 126 897
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 557 1023
Size: 447 110 466
Fragments arranged by size:
466 447 110
PfoI T'CCnGG_A
Cuts at: 0 197 1023
Size: 197 826
PpuMI rG'GwC_Cy
Cuts at: 0 433 1023
Size: 433 590
PshAI GACnn'nnGTC
Cuts at: 0 882 1023
Size: 882 141
PstI C_TGCA'G
Cuts at: 0 122 1023
Size: 122 901
PvuII CAG'CTG
Cuts at: 0 734 1023
Size: 734 289
SacI G_AGCT'C
Cuts at: 0 197 1023
Size: 197 826
SfcI C'TryA_G
Cuts at: 0 118 1023
Size: 118 905
SmlI C'TyrA_G
Cuts at: 0 609 630 1023
Size: 609 21 393
Fragments arranged by size:
609 393 21
StuI AGG'CCT
Cuts at: 0 485 1023
Size: 485 538
StyI C'CwwG_G
Cuts at: 0 134 272 1023
Size: 134 138 751
Fragments arranged by size:
751 138 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 536 1023
Size: 536 487
TatI w'GTAC_w
Cuts at: 0 425 443 621 1023
Size: 425 18 178 402
Fragments arranged by size:
425 402 178 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 362 394 1023
Size: 362 32 629
Fragments arranged by size:
629 362 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 1023
Size: 279 744
XmnI GAAnn'nnTTC
Cuts at: 0 319 1023
Size: 319 704
Enzymes that do cut:
AcuI AflIII AlwNI BanII BbsI BmrI BpmI Bpu10I
BpuEI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrDI
Bsu36I BtgI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I
HincII MmeI MslI MspA1I NcoI NspI PciI PflMI
PfoI PpuMI PshAI PstI PvuII SacI SfcI SmlI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI