(Linear) (Six-base) MAPSORT of: KIEE3956.seq  Check: 4405  from: 1  to: 1533



KIEE3956.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     465    1533 
   Size:        465    1068
 
AccI GT'mk_AC

Cuts at:      0     630     644    1533 
   Size:        630      14     889

  Fragments arranged by size: 

                 889     630      14
 
AfeI AGC'GCT

Cuts at:      0     302    1533 
   Size:        302    1231
 
AlwNI CAG_nnn'CTG

Cuts at:      0     211    1533 
   Size:        211    1322
 
ApoI r'AATT_y

Cuts at:      0     695    1069    1194    1419    1533 
   Size:        695     374     125     225     114

  Fragments arranged by size: 

                 695     374     225     125     114
 
AseI AT'TA_AT

Cuts at:      0     596    1533 
   Size:        596     937
 
AvaI C'yCGr_G

Cuts at:      0     272    1533 
   Size:        272    1261
 
BanI G'GyrC_C

Cuts at:      0     167    1533 
   Size:        167    1366
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1009    1083    1402    1533 
   Size:       1009      74     319     131

  Fragments arranged by size: 

                1009     319     131      74
 
BclI T'GATC_A

Cuts at:      0    1365    1533 
   Size:       1365     168
 
BfrBI ATG'CAT

Cuts at:      0     475    1533 
   Size:        475    1058
 
BglII A'GATC_T

Cuts at:      0    1215    1533 
   Size:       1215     318
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     775     813    1533 
   Size:        775      38     720

  Fragments arranged by size: 

                 775     720      38
 
Bpu10I CC'TnA_GC

Cuts at:      0     866    1533 
   Size:        866     667
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745    1533 
   Size:        745     788
 
BsaAI yAC'GTr

Cuts at:      0     107    1533 
   Size:        107    1426
 
BsaHI Gr'CG_yC

Cuts at:      0     627    1533 
   Size:        627     906
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     567     597    1533 
   Size:        567      30     936

  Fragments arranged by size: 

                 936     567      30
 
BseYI C'CCAG_C

Cuts at:      0    1028    1533 
   Size:       1028     505
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31    1533 
   Size:         31    1502
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     465    1533 
   Size:        465    1068
 
BstYI r'GATC_y

Cuts at:      0    1215    1323    1533 
   Size:       1215     108     210

  Fragments arranged by size: 

                1215     210     108
 
BtgI C'CryG_G

Cuts at:      0     722    1533 
   Size:        722     811
 
DraI TTT'AAA

Cuts at:      0     176     539    1122    1533 
   Size:        176     363     583     411

  Fragments arranged by size: 

                 583     411     363     176
 
EaeI y'GGCC_r

Cuts at:      0       2    1304    1533 
   Size:          2    1302     229

  Fragments arranged by size: 

                1302     229       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     140    1314    1533 
   Size:        140    1174     219

  Fragments arranged by size: 

                1174     219     140
 
EcoO109I rG'GnC_Cy

Cuts at:      0     516    1533 
   Size:        516    1017
 
EcoRV GAT'ATC

Cuts at:      0     495    1533 
   Size:        495    1038
 
HaeII r_GCGC'y

Cuts at:      0     304    1533 
   Size:        304    1229
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     279     311     484     516    1350    1382    1533 
   Size:        279      32     173      32     834      32     151

  Fragments arranged by size: 

                 834     279     173     151      32      32      32
 
HincII GTy'rAC

Cuts at:      0     729    1185    1533 
   Size:        729     456     348

  Fragments arranged by size: 

                 729     456     348
 
HindIII A'AGCT_T

Cuts at:      0     858    1042    1297    1533 
   Size:        858     184     255     236

  Fragments arranged by size: 

                 858     255     236     184
 
MfeI C'AATT_G

Cuts at:      0     620     806    1533 
   Size:        620     186     727

  Fragments arranged by size: 

                 727     620     186
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     358    1406    1533 
   Size:        358    1048     127

  Fragments arranged by size: 

                1048     358     127
 
MscI TGG'CCA

Cuts at:      0       4    1306    1533 
   Size:          4    1302     227

  Fragments arranged by size: 

                1302     227       4
 
NcoI C'CATG_G

Cuts at:      0     722    1533 
   Size:        722     811
 
NsiI A_TGCA'T

Cuts at:      0     477    1533 
   Size:        477    1056
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     840    1533 
   Size:        840     693
 
PfoI T'CCnGG_A

Cuts at:      0    1266    1533 
   Size:       1266     267
 
PpuMI rG'GwC_Cy

Cuts at:      0     516    1533 
   Size:        516    1017
 
PspXI vC'TCGA_Gb

Cuts at:      0     272    1533 
   Size:        272    1261
 
SmlI C'TyrA_G

Cuts at:      0     272     760    1533 
   Size:        272     488     773

  Fragments arranged by size: 

                 773     488     272
 
StuI AGG'CCT

Cuts at:      0      66    1533 
   Size:         66    1467
 
StyI C'CwwG_G

Cuts at:      0     722    1533 
   Size:        722     811
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     893    1517    1533 
   Size:        893     624      16

  Fragments arranged by size: 

                 893     624      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     775     807    1533 
   Size:        775      32     726

  Fragments arranged by size: 

                 775     726      32
 
XhoI C'TCGA_G

Cuts at:      0     272    1533 
   Size:        272    1261
 
XmnI GAAnn'nnTTC

Cuts at:      0     329     824    1533 
   Size:        329     495     709

  Fragments arranged by size: 

                 709     495     329
 

 Enzymes that do cut:

     AarI     AccI     AfeI    AlwNI     ApoI     AseI     AvaI     BanI
     BbsI     BclI    BfrBI    BglII     BplI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaXI    BseYI     BsgI    BspMI    BstYI     BtgI     DraI
     EaeI     EarI EcoO109I    EcoRV    HaeII    Hin4I   HincII  HindIII
     MfeI     MmeI     MscI     NcoI     NsiI    PflMI     PfoI    PpuMI
    PspXI     SmlI     StuI     StyI    TaqII     TstI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI     BsaI    BsaBI
    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI     FalI     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MluI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I
     XbaI     XcmI     XmaI     ZraI