Restriction map for
pFN21AE3954
(Linear) (Six-base) MAPSORT of: KIEE3954.seq Check: 5465 from: 1 to: 1848
KIEE3954.orf
With 182 enzymes: SgfI *
March 22, 2012 16:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 380 952 1686 1848
Size: 380 572 734 162
Fragments arranged by size:
734 572 380 162
AatII G_ACGT'C
Cuts at: 0 143 1848
Size: 143 1705
AccI GT'mk_AC
Cuts at: 0 65 395 584 1736 1848
Size: 65 330 189 1152 112
Fragments arranged by size:
1152 330 189 112 65
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 1848
Size: 1220 628
AfeI AGC'GCT
Cuts at: 0 1229 1528 1848
Size: 1229 299 320
Fragments arranged by size:
1229 320 299
AflIII A'CryG_T
Cuts at: 0 1412 1848
Size: 1412 436
AhdI GACnn_n'nnGTC
Cuts at: 0 1250 1269 1848
Size: 1250 19 579
Fragments arranged by size:
1250 579 19
AleI CACnn'nnGTG
Cuts at: 0 1013 1848
Size: 1013 835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 112 619 883 1402 1848
Size: 112 507 264 519 446
Fragments arranged by size:
519 507 446 264 112
AlwNI CAG_nnn'CTG
Cuts at: 0 105 459 576 651 693 1848
Size: 105 354 117 75 42 1155
Fragments arranged by size:
1155 354 117 105 75 42
AvaI C'yCGr_G
Cuts at: 0 664 1344 1848
Size: 664 680 504
Fragments arranged by size:
680 664 504
BanI G'GyrC_C
Cuts at: 0 271 1315 1587 1829 1848
Size: 271 1044 272 242 19
Fragments arranged by size:
1044 272 271 242 19
BanII G_rGCy'C
Cuts at: 0 131 290 932 1848
Size: 131 159 642 916
Fragments arranged by size:
916 642 159 131
BbeI G_GCGC'C
Cuts at: 0 275 1591 1848
Size: 275 1316 257
Fragments arranged by size:
1316 275 257
BbsI GAAGACnn'nnnn_
Cuts at: 0 1193 1848
Size: 1193 655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1716 1750 1848
Size: 1716 34 98
Fragments arranged by size:
1716 98 34
BglI GCCn_nnn'nGGC
Cuts at: 0 999 1313 1848
Size: 999 314 535
Fragments arranged by size:
999 535 314
BglII A'GATC_T
Cuts at: 0 380 1848
Size: 380 1468
Bme1580I G_kGCm'C
Cuts at: 0 1318 1848
Size: 1318 530
BmgBI CAC'GTC
Cuts at: 0 1413 1536 1848
Size: 1413 123 312
Fragments arranged by size:
1413 312 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 480 1848
Size: 480 1368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 436 715 946 1804 1848
Size: 379 57 279 231 858 44
Fragments arranged by size:
858 379 279 231 57 44
Bpu10I CC'TnA_GC
Cuts at: 0 589 1848
Size: 589 1259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 274 463 1848
Size: 69 205 189 1385
Fragments arranged by size:
1385 205 189 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 915 1848
Size: 915 933
BsaAI yAC'GTr
Cuts at: 0 1301 1848
Size: 1301 547
BsaHI Gr'CG_yC
Cuts at: 0 140 272 956 985 1588 1733 1848
Size: 140 132 684 29 603 145 115
Fragments arranged by size:
684 603 145 140 132 115 29
BsaWI w'CCGG_w
Cuts at: 0 1403 1848
Size: 1403 445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 1162 1192 1848
Size: 199 30 933 30 656
Fragments arranged by size:
933 656 199 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 817 1435 1848
Size: 817 618 413
Fragments arranged by size:
817 618 413
BseYI C'CCAG_C
Cuts at: 0 71 836 1026 1539 1664 1848
Size: 71 765 190 513 125 184
Fragments arranged by size:
765 513 190 184 125 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 625 721 1485 1714 1848
Size: 219 406 96 764 229 134
Fragments arranged by size:
764 406 229 219 134 96
BsiEI CG_ry'CG
Cuts at: 0 755 1212 1707 1848
Size: 755 457 495 141
Fragments arranged by size:
755 495 457 141
BsiHKAI G_wGCw'C
Cuts at: 0 623 763 932 1848
Size: 623 140 169 916
Fragments arranged by size:
916 623 169 140
BsiWI C'GTAC_G
Cuts at: 0 1301 1848
Size: 1301 547
BsmBI CGTCTCn'nnnn_
Cuts at: 0 124 355 567 976 1237 1848
Size: 124 231 212 409 261 611
Fragments arranged by size:
611 409 261 231 212 124
Bsp1286I G_dGCh'C
Cuts at: 0 131 290 623 763 932 1318 1848
Size: 131 159 333 140 169 386 530
Fragments arranged by size:
530 386 333 169 159 140 131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 380 458 740 952 1394 1686 1748
Size: 194 186 78 282 212 442 292 62
Cuts at: 1748 1848
Size: 100
Fragments arranged by size:
442 292 282 212 194 186 100 78
62
BsrBI CCG'CTC
Cuts at: 0 714 918 1425 1848
Size: 714 204 507 423
Fragments arranged by size:
714 507 423 204
BsrFI r'CCGG_y
Cuts at: 0 750 1048 1282 1848
Size: 750 298 234 566
Fragments arranged by size:
750 566 298 234
BsrGI T'GTAC_A
Cuts at: 0 1337 1848
Size: 1337 511
BssHII G'CGCG_C
Cuts at: 0 1444 1848
Size: 1444 404
BstAPI GCAn_nnn'nTGC
Cuts at: 0 168 1848
Size: 168 1680
BstYI r'GATC_y
Cuts at: 0 380 1848
Size: 380 1468
BstZ17I GTA'TAC
Cuts at: 0 66 1848
Size: 66 1782
BtgI C'CryG_G
Cuts at: 0 123 296 1439 1520 1789 1848
Size: 123 173 1143 81 269 59
Fragments arranged by size:
1143 269 173 123 81 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 163 1029 1517 1737 1848
Size: 163 866 488 220 111
Fragments arranged by size:
866 488 220 163 111
BtsI GCAGTG_nn'
Cuts at: 0 652 976 1570 1631 1673 1848
Size: 652 324 594 61 42 175
Fragments arranged by size:
652 594 324 175 61 42
ClaI AT'CG_AT
Cuts at: 0 500 1848
Size: 500 1348
DrdI GACnn_nn'nnGTC
Cuts at: 0 27 1848
Size: 27 1821
EaeI y'GGCC_r
Cuts at: 0 34 152 191 752 1199 1209 1280 1305
Size: 34 118 39 561 447 10 71 25
Cuts at: 1305 1792 1848
Size: 487 56
Fragments arranged by size:
561 487 447 118 71 56 39 34
25 10
EagI C'GGCC_G
Cuts at: 0 752 1209 1848
Size: 752 457 639
Fragments arranged by size:
752 639 457
EarI CTCTTCn'nnn_
Cuts at: 0 136 1239 1848
Size: 136 1103 609
Fragments arranged by size:
1103 609 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1017 1755 1848
Size: 1017 738 93
Fragments arranged by size:
1017 738 93
EcoICRI GAG'CTC
Cuts at: 0 930 1848
Size: 930 918
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 436 715 946 1220 1804 1848
Size: 379 57 279 231 274 584 44
Fragments arranged by size:
584 379 279 274 231 57 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 418 631 1744 1848
Size: 418 213 1113 104
Fragments arranged by size:
1113 418 213 104
FspI TGC'GCA
Cuts at: 0 871 1848
Size: 871 977
HaeII r_GCGC'y
Cuts at: 0 275 868 888 1158 1231 1530 1591 1848
Size: 275 593 20 270 73 299 61 257
Fragments arranged by size:
593 299 275 270 257 73 61 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1072 1104 1236 1255 1268 1287 1848
Size: 1072 32 132 19 13 19 561
Fragments arranged by size:
1072 561 132 32 19 19 13
HincII GTy'rAC
Cuts at: 0 1737 1848
Size: 1737 111
HindIII A'AGCT_T
Cuts at: 0 242 1848
Size: 242 1606
KasI G'GCGC_C
Cuts at: 0 271 1587 1848
Size: 271 1316 261
Fragments arranged by size:
1316 271 261
MscI TGG'CCA
Cuts at: 0 1201 1848
Size: 1201 647
MslI CAynn'nnrTG
Cuts at: 0 232 1013 1195 1848
Size: 232 781 182 653
Fragments arranged by size:
781 653 232 182
MspA1I CmG'CkG
Cuts at: 0 71 102 125 609 990 1118 1593 1664
Size: 71 31 23 484 381 128 475 71
Cuts at: 1664 1848
Size: 184
Fragments arranged by size:
484 475 381 184 128 71 71 31
23
NaeI GCC'GGC
Cuts at: 0 752 1050 1284 1848
Size: 752 298 234 564
Fragments arranged by size:
752 564 298 234
NarI GG'CG_CC
Cuts at: 0 272 1588 1848
Size: 272 1316 260
Fragments arranged by size:
1316 272 260
NcoI C'CATG_G
Cuts at: 0 1520 1848
Size: 1520 328
NgoMIV G'CCGG_C
Cuts at: 0 750 1048 1282 1848
Size: 750 298 234 566
Fragments arranged by size:
750 566 298 234
NspI r_CATG'y
Cuts at: 0 237 1194 1464 1603 1848
Size: 237 957 270 139 245
Fragments arranged by size:
957 270 245 237 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 693 1848
Size: 693 1155
PfoI T'CCnGG_A
Cuts at: 0 356 1848
Size: 356 1492
PstI C_TGCA'G
Cuts at: 0 101 380 455 557 635 647 1848
Size: 101 279 75 102 78 12 1201
Fragments arranged by size:
1201 279 102 101 78 75 12
PvuII CAG'CTG
Cuts at: 0 1664 1848
Size: 1664 184
SacI G_AGCT'C
Cuts at: 0 932 1848
Size: 932 916
SacII CC_GC'GG
Cuts at: 0 126 1848
Size: 126 1722
SalI G'TCGA_C
Cuts at: 0 1735 1848
Size: 1735 113
SapI GCTCTTCn'nnn_
Cuts at: 0 136 1848
Size: 136 1712
SbfI CC_TGCA'GG
Cuts at: 0 455 635 1848
Size: 455 180 1213
Fragments arranged by size:
1213 455 180
SfcI C'TryA_G
Cuts at: 0 97 376 451 471 553 631 643 1554
Size: 97 279 75 20 82 78 12 911
Cuts at: 1554 1659 1848
Size: 105 189
Fragments arranged by size:
911 279 189 105 97 82 78 75
20 12
SfoI GGC'GCC
Cuts at: 0 273 1589 1848
Size: 273 1316 259
Fragments arranged by size:
1316 273 259
SgrAI Cr'CCGG_yG
Cuts at: 0 1048 1848
Size: 1048 800
SmlI C'TyrA_G
Cuts at: 0 84 289 478 1848
Size: 84 205 189 1370
Fragments arranged by size:
1370 205 189 84
SphI G_CATG'C
Cuts at: 0 237 1603 1848
Size: 237 1366 245
Fragments arranged by size:
1366 245 237
StuI AGG'CCT
Cuts at: 0 1552 1848
Size: 1552 296
StyI C'CwwG_G
Cuts at: 0 773 1291 1520 1745 1848
Size: 773 518 229 225 103
Fragments arranged by size:
773 518 229 225 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1462 1848
Size: 1462 386
TatI w'GTAC_w
Cuts at: 0 1337 1848
Size: 1337 511
Tth111I GACn'n_nGTC
Cuts at: 0 142 352 1848
Size: 142 210 1496
Fragments arranged by size:
1496 210 142
XmnI GAAnn'nnTTC
Cuts at: 0 520 1848
Size: 520 1328
ZraI GAC'GTC
Cuts at: 0 141 1848
Size: 141 1707
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI AvaI BanI BanII BbeI BbsI BcgI
BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstYI BstZ17I BtgI BtgZI BtsI
ClaI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI FspI HaeII Hin4I HincII HindIII KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI
PfoI PstI PvuII SacI SacII SalI SapI SbfI
SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII
TatI Tth111I XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BlpI BmrI BmtI BsaBI BsmI BspEI
BspHI BsrDI BssSI BstBI BstEII BstXI Bsu36I CspCI
DraI DraIII EcoO109I EcoRI EcoRV FalI FseI FspAI
HpaI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SanDI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI
SrfI SspI SwaI TstI XbaI XcmI XhoI XmaI